BRENDA - Enzyme Database
show all sequences of 1.14.13.217

Reevaluation of the violacein biosynthetic pathway and its relationship to indolocarbazole biosynthesis

Sanchez, C.; Brana, A.F.; Mendez, C.; Salas, J.A.; ChemBioChem 7, 1231-1240 (2006)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
NADPH
-
Chromobacterium violaceum
Cloned(Commentary)
Cloned (Commentary)
Organism
gene vioD, recombinant expression in Escherichia coli strain DH10B and Streptomyces albus strain J1074, coexpression with the other genes of the violacein biosynthesis pathway, reevaluation, overview
Chromobacterium violaceum
Engineering
Protein Variants
Commentary
Organism
additional information
additional coexpression of vioC and/or vioD in Streptomyces albus. Disruption of vioA or vioB completely abrogates the biosynthesis of violacein intermediates, while disruption of vioC or vioD results in the production of violacein precursors
Chromobacterium violaceum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Chromobacterium violaceum
VioD is no tryptophan 5-hydroxylase
?
-
-
?
additional information
Chromobacterium violaceum JCM 1249
VioD is no tryptophan 5-hydroxylase
?
-
-
?
protodeoxyviolaceinate + NAD(P)H + H+ + O2
Chromobacterium violaceum
-
protoviolaceinate + NAD(P)+
-
-
?
protodeoxyviolaceinate + NAD(P)H + H+ + O2
Chromobacterium violaceum JCM 1249
-
protoviolaceinate + NAD(P)+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Chromobacterium violaceum
Q9S3U8
strain JCM1249 or ATCC12472
-
Chromobacterium violaceum JCM 1249
Q9S3U8
strain JCM1249 or ATCC12472
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
VioD is no tryptophan 5-hydroxylase
735954
Chromobacterium violaceum
?
-
-
-
?
additional information
VioD is no tryptophan 5-hydroxylase
735954
Chromobacterium violaceum JCM 1249
?
-
-
-
?
protodeoxyviolaceinate + NAD(P)H + H+ + O2
-
735954
Chromobacterium violaceum
protoviolaceinate + NAD(P)+
-
-
-
?
protodeoxyviolaceinate + NAD(P)H + H+ + O2
-
735954
Chromobacterium violaceum JCM 1249
protoviolaceinate + NAD(P)+
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
VioD
-
Chromobacterium violaceum
VioD oxygenase
-
Chromobacterium violaceum
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Chromobacterium violaceum
NADPH
-
Chromobacterium violaceum
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
NADPH
-
Chromobacterium violaceum
Cloned(Commentary) (protein specific)
Commentary
Organism
gene vioD, recombinant expression in Escherichia coli strain DH10B and Streptomyces albus strain J1074, coexpression with the other genes of the violacein biosynthesis pathway, reevaluation, overview
Chromobacterium violaceum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Chromobacterium violaceum
NADPH
-
Chromobacterium violaceum
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
additional coexpression of vioC and/or vioD in Streptomyces albus. Disruption of vioA or vioB completely abrogates the biosynthesis of violacein intermediates, while disruption of vioC or vioD results in the production of violacein precursors
Chromobacterium violaceum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Chromobacterium violaceum
VioD is no tryptophan 5-hydroxylase
?
-
-
?
additional information
Chromobacterium violaceum JCM 1249
VioD is no tryptophan 5-hydroxylase
?
-
-
?
protodeoxyviolaceinate + NAD(P)H + H+ + O2
Chromobacterium violaceum
-
protoviolaceinate + NAD(P)+
-
-
?
protodeoxyviolaceinate + NAD(P)H + H+ + O2
Chromobacterium violaceum JCM 1249
-
protoviolaceinate + NAD(P)+
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
VioD is no tryptophan 5-hydroxylase
735954
Chromobacterium violaceum
?
-
-
-
?
additional information
VioD is no tryptophan 5-hydroxylase
735954
Chromobacterium violaceum JCM 1249
?
-
-
-
?
protodeoxyviolaceinate + NAD(P)H + H+ + O2
-
735954
Chromobacterium violaceum
protoviolaceinate + NAD(P)+
-
-
-
?
protodeoxyviolaceinate + NAD(P)H + H+ + O2
-
735954
Chromobacterium violaceum JCM 1249
protoviolaceinate + NAD(P)+
-
-
-
?
General Information
General Information
Commentary
Organism
evolution
VioD belongs to the family of flavin-containing NAD(P)H-dependent monooxygenases
Chromobacterium violaceum
malfunction
disruption of vioA or vioB completely abrogates the biosynthesis of violacein intermediates, while disruption of vioC or vioD results in the production of violacein precursors. Gene vioE is essential for violacein biosynthesis. Enzymes VioC and VioD are responsible for the oxygenations at the ox-indole and hydroxyindole, respectively. In the absence of VioC, the expected proviolaceins are accompanied by other compounds: deoxychromoviridans, chromoviridans, and oxychromoviridans
Chromobacterium violaceum
metabolism
vioD is one gene encoding violacein biosynthesis, VioD appears to act on late steps of violacein biosynthesis, overview
Chromobacterium violaceum
General Information (protein specific)
General Information
Commentary
Organism
evolution
VioD belongs to the family of flavin-containing NAD(P)H-dependent monooxygenases
Chromobacterium violaceum
malfunction
disruption of vioA or vioB completely abrogates the biosynthesis of violacein intermediates, while disruption of vioC or vioD results in the production of violacein precursors. Gene vioE is essential for violacein biosynthesis. Enzymes VioC and VioD are responsible for the oxygenations at the ox-indole and hydroxyindole, respectively. In the absence of VioC, the expected proviolaceins are accompanied by other compounds: deoxychromoviridans, chromoviridans, and oxychromoviridans
Chromobacterium violaceum
metabolism
vioD is one gene encoding violacein biosynthesis, VioD appears to act on late steps of violacein biosynthesis, overview
Chromobacterium violaceum
Other publictions for EC 1.14.13.217
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735402
Ran
Expression, crystallization an ...
Chromobacterium violaceum
Acta crystallogr. Sect. F
71
149-152
2015
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1
1
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736735
Rodrigues
Systems metabolic engineering ...
Janthinobacterium lividum, Janthinobacterium lividum DSM 1522
Metab. Eng.
20
29-41
2013
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735532
Jiang
Pathway redesign for deoxyviol ...
Duganella sp., Duganella sp. B2
Appl. Microbiol. Biotechnol.
94
1521-1532
2012
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735934
Shinoda
Biosynthesis of violacein: A g ...
Chromobacterium violaceum, Chromobacterium violaceum JCM 1249
Chem. Commun. (Camb.)
2007
4140-4142
2007
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2
2
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735666
Balibar
In vitro biosynthesis of viola ...
Chromobacterium violaceum, Chromobacterium violaceum ATCC 12472
Biochemistry
45
15444-15457
2006
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1
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735954
Sanchez
Reevaluation of the violacein ...
Chromobacterium violaceum, Chromobacterium violaceum JCM 1249
ChemBioChem
7
1231-1240
2006
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3
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736665
August
Sequence analysis and function ...
Chromobacterium violaceum
J. Mol. Microbiol. Biotechnol.
2
513-519
2000
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