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Literature summary for 1.14.13.211 extracted from

  • Hoshino, Y.;, Fujii, S., Shinonaga, H.; Arai, K.; Saito, F.; Fukai, T.; Satoh, H.; Miyazaki, Y.; Ishikawa, J.
    Monooxygenation of rifampicin catalyzed by the rox gene product of Nocardia farcinica: structure elucidation, gene identification and role in drug resistance (2010), J. Antibiot., 63, 23-28.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene rox, cloning and expression of wild-type and mutant enzymes in Escherichia coli strains JM109 and BL21(DE3) Nocardia farcinica

Protein Variants

Protein Variants Comment Organism
additional information the rox gene deletion mutation DELTArox gives no significant influence to the rifampicin resistance of Nocardia farcinica. Transformation with plasmid pKNL027_N05 overexpressing the rox gene markedly raises the rifampicin resistance in the strain with the deletion mutation of the rpoB2 gene as the principal rifampicin resistance determinant. Rifampicin is degraded by the wild-type strain, whereas it remains intact when incubated with the DELTArox strain Nocardia farcinica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
rifampicin + NAD(P)H + O2 Nocardia farcinica
-
2'-N-hydroxyrifampicin + NAD(P)+ + H2O
-
?
rifampicin + NAD(P)H + O2 Nocardia farcinica IFM 10152
-
2'-N-hydroxyrifampicin + NAD(P)+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Nocardia farcinica
-
gene rox
-
Nocardia farcinica IFM 10152
-
gene rox
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
rifampicin + NAD(P)H + O2
-
Nocardia farcinica 2'-N-hydroxyrifampicin + NAD(P)+ + H2O
-
?
rifampicin + NAD(P)H + O2
-
Nocardia farcinica IFM 10152 2'-N-hydroxyrifampicin + NAD(P)+ + H2O
-
?

Synonyms

Synonyms Comment Organism
rifampicin monooxygenase
-
Nocardia farcinica
rox
-
Nocardia farcinica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
27
-
assay at Nocardia farcinica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Nocardia farcinica

Cofactor

Cofactor Comment Organism Structure
FAD enzyme Rox is a probable FAD-dependent monooxygenase because it contains the FAD-binding domain (Pfam accession number PF01494) Nocardia farcinica
NAD(P)H
-
Nocardia farcinica

General Information

General Information Comment Organism
physiological function the rox gene is capable of initiating rifampicin degradation with another metabolite formation at the first step and having a role as the secondary rifampicin resistance factor in Nocardia farcinica Nocardia farcinica