Cloned (Comment) | Organism |
---|---|
gene rox, cloning and expression of wild-type and mutant enzymes in Escherichia coli strains JM109 and BL21(DE3) | Nocardia farcinica |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the rox gene deletion mutation DELTArox gives no significant influence to the rifampicin resistance of Nocardia farcinica. Transformation with plasmid pKNL027_N05 overexpressing the rox gene markedly raises the rifampicin resistance in the strain with the deletion mutation of the rpoB2 gene as the principal rifampicin resistance determinant. Rifampicin is degraded by the wild-type strain, whereas it remains intact when incubated with the DELTArox strain | Nocardia farcinica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
rifampicin + NAD(P)H + O2 | Nocardia farcinica | - |
2'-N-hydroxyrifampicin + NAD(P)+ + H2O | - |
? | |
rifampicin + NAD(P)H + O2 | Nocardia farcinica IFM 10152 | - |
2'-N-hydroxyrifampicin + NAD(P)+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nocardia farcinica | - |
gene rox | - |
Nocardia farcinica IFM 10152 | - |
gene rox | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
rifampicin + NAD(P)H + O2 | - |
Nocardia farcinica | 2'-N-hydroxyrifampicin + NAD(P)+ + H2O | - |
? | |
rifampicin + NAD(P)H + O2 | - |
Nocardia farcinica IFM 10152 | 2'-N-hydroxyrifampicin + NAD(P)+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
rifampicin monooxygenase | - |
Nocardia farcinica |
rox | - |
Nocardia farcinica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
27 | - |
assay at | Nocardia farcinica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Nocardia farcinica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | enzyme Rox is a probable FAD-dependent monooxygenase because it contains the FAD-binding domain (Pfam accession number PF01494) | Nocardia farcinica | |
NAD(P)H | - |
Nocardia farcinica |
General Information | Comment | Organism |
---|---|---|
physiological function | the rox gene is capable of initiating rifampicin degradation with another metabolite formation at the first step and having a role as the secondary rifampicin resistance factor in Nocardia farcinica | Nocardia farcinica |