Monooxygenation of rifampicin catalyzed by the rox gene product of Nocardia farcinica: structure elucidation, gene identification and role in drug resistance
Hoshino, Y.;, Fujii, S., Shinonaga, H.; Arai, K.; Saito, F.; Fukai, T.; Satoh, H.; Miyazaki, Y.; Ishikawa, J.; J. Antibiot. 63, 23-28 (2010) 
Data extracted from this reference:
Cloned(Commentary)
Cloned (Commentary)
Organism
gene rox, cloning and expression of wild-type and mutant enzymes in Escherichia coli strains JM109 and BL21(DE3)
Nocardia farcinica
Engineering
Protein Variants
Commentary
Organism
additional information
the rox gene deletion mutation DELTArox gives no significant influence to the rifampicin resistance of Nocardia farcinica. Transformation with plasmid pKNL027_N05 overexpressing the rox gene markedly raises the rifampicin resistance in the strain with the deletion mutation of the rpoB2 gene as the principal rifampicin resistance determinant. Rifampicin is degraded by the wild-type strain, whereas it remains intact when incubated with the DELTArox strain
Nocardia farcinica
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
rifampicin + NAD(P)H + O2
Nocardia farcinica
-
2'-N-hydroxyrifampicin + NAD(P)+ + H2O
-
-
?
rifampicin + NAD(P)H + O2
Nocardia farcinica IFM 10152
-
2'-N-hydroxyrifampicin + NAD(P)+ + H2O
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Nocardia farcinica
-
gene rox
-
Nocardia farcinica IFM 10152
-
gene rox
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
rifampicin + NAD(P)H + O2
-
734035
Nocardia farcinica
2'-N-hydroxyrifampicin + NAD(P)+ + H2O
-
-
-
?
rifampicin + NAD(P)H + O2
-
734035
Nocardia farcinica IFM 10152
2'-N-hydroxyrifampicin + NAD(P)+ + H2O
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
rifampicin monooxygenase
-
Nocardia farcinica
rox
-
Nocardia farcinica
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
27
-
assay at
Nocardia farcinica
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Nocardia farcinica
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
enzyme Rox is a probable FAD-dependent monooxygenase because it contains the FAD-binding domain (Pfam accession number PF01494)
Nocardia farcinica
NAD(P)H
-
Nocardia farcinica
Cloned(Commentary) (protein specific)
Commentary
Organism
gene rox, cloning and expression of wild-type and mutant enzymes in Escherichia coli strains JM109 and BL21(DE3)
Nocardia farcinica
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
enzyme Rox is a probable FAD-dependent monooxygenase because it contains the FAD-binding domain (Pfam accession number PF01494)
Nocardia farcinica
NAD(P)H
-
Nocardia farcinica
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
the rox gene deletion mutation DELTArox gives no significant influence to the rifampicin resistance of Nocardia farcinica. Transformation with plasmid pKNL027_N05 overexpressing the rox gene markedly raises the rifampicin resistance in the strain with the deletion mutation of the rpoB2 gene as the principal rifampicin resistance determinant. Rifampicin is degraded by the wild-type strain, whereas it remains intact when incubated with the DELTArox strain
Nocardia farcinica
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
rifampicin + NAD(P)H + O2
Nocardia farcinica
-
2'-N-hydroxyrifampicin + NAD(P)+ + H2O
-
-
?
rifampicin + NAD(P)H + O2
Nocardia farcinica IFM 10152
-
2'-N-hydroxyrifampicin + NAD(P)+ + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
rifampicin + NAD(P)H + O2
-
734035
Nocardia farcinica
2'-N-hydroxyrifampicin + NAD(P)+ + H2O
-
-
-
?
rifampicin + NAD(P)H + O2
-
734035
Nocardia farcinica IFM 10152
2'-N-hydroxyrifampicin + NAD(P)+ + H2O
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
27
-
assay at
Nocardia farcinica
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Nocardia farcinica
General Information
General Information
Commentary
Organism
physiological function
the rox gene is capable of initiating rifampicin degradation with another metabolite formation at the first step and having a role as the secondary rifampicin resistance factor in Nocardia farcinica
Nocardia farcinica
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the rox gene is capable of initiating rifampicin degradation with another metabolite formation at the first step and having a role as the secondary rifampicin resistance factor in Nocardia farcinica
Nocardia farcinica
Other publictions for EC 1.14.13.211
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738694
Liu
The structure of the antibioti ...
Nocardia farcinica, Nocardia farcinica IFM 10152
J. Biol. Chem.
291
21553-21562
2016
-
-
1
1
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
734035
Hoshino
Monooxygenation of rifampicin ...
Nocardia farcinica, Nocardia farcinica IFM 10152
J. Antibiot.
63
23-28
2010
-
-
1
-
1
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
2
-
2
1
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
735440
Andersen
Monooxygenase-like sequence of ...
Rhodococcus hoagii, Rhodococcus hoagii ATCC 14887
Antimicrob. Agents Chemother.
41
218-221
1997
-
-
1
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-