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Literature summary for 1.14.13.20 extracted from
- Purification and characterization of 2,4-dichlorophenol hydroxylase isolated from a bacterium of the alpha-2 subgroup of the Proteobacteria (1997), FEMS Microbiol. Lett., 157, 95-101.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
2,4-Dichlorophenol | - |
Proteobacteria |  |
| 0.024 | - |
NADPH | - |
Proteobacteria | |
| 0.042 | - |
NADH | - |
Proteobacteria | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
alpha4, 4 * 64000, SDS-PAGE | Proteobacteria |
| 256000 | - |
gel filtration, gradient PAGE | Proteobacteria |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,4-dichlorophenol + NADPH + O2 | Proteobacteria | - |
3,5-dichlorocatechol + NADP+ + H2O | - |
? | |
| 2,4-dichlorophenol + NADPH + O2 | Proteobacteria S1 | - |
3,5-dichlorocatechol + NADP+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Proteobacteria | - |
S1 | - |
| Proteobacteria S1 | - |
S1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Proteobacteria |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.8 | - |
without FAD | Proteobacteria |
| 2.4 | - |
- |
Proteobacteria |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,3-dichlorophenol + NADPH + O2 | - |
Proteobacteria | ? | - |
? | |
| 2,3-dichlorophenol + NADPH + O2 | - |
Proteobacteria S1 | ? | - |
? | |
| 2,4,5-trichlorophenol + NADPH + O2 | - |
Proteobacteria | ? | - |
? | |
| 2,4,5-trichlorophenol + NADPH + O2 | - |
Proteobacteria S1 | ? | - |
? | |
| 2,4,6-trichlorophenol + NADPH + O2 | - |
Proteobacteria | ? | - |
? | |
| 2,4-dibromophenol + NADPH + O2 | - |
Proteobacteria | ? | - |
? | |
| 2,4-dibromophenol + NADPH + O2 | - |
Proteobacteria S1 | ? | - |
? | |
| 2,4-dichlorophenol + NADH + H+ + O2 | - |
Proteobacteria | 3,5-dichlorocatechol + NAD+ + H2O | - |
r | |
| 2,4-dichlorophenol + NADPH + O2 | - |
Proteobacteria | 3,5-dichlorocatechol + NADP+ + H2O | - |
? | |
| 2,4-dichlorophenol + NADPH + O2 | - |
Proteobacteria S1 | 3,5-dichlorocatechol + NADP+ + H2O | - |
? | |
| 2,5-dichlorophenol + NADPH + O2 | - |
Proteobacteria | ? | - |
? | |
| 2,6-dichlorophenol + NADPH + O2 | - |
Proteobacteria | ? | - |
? | |
| 2-chlorophenol + NADPH + O2 | - |
Proteobacteria | ? | - |
? | |
| 2-methylphenol + NADPH + O2 | - |
Proteobacteria | ? | - |
? | |
| 3,4-dichlorophenol + NADPH + O2 | - |
Proteobacteria | ? | - |
? | |
| 3,5-dichlorophenol + NADPH + O2 | - |
Proteobacteria | ? | - |
? | |
| 3-chlorophenol + NADPH + O2 | - |
Proteobacteria | ? | - |
? | |
| 4-bromophenol + NADPH + O2 | - |
Proteobacteria | ? | - |
? | |
| 4-chloro-2-methylphenol + NADPH + O2 | - |
Proteobacteria | ? | - |
? | |
| 4-chloro-2-methylphenol + NADPH + O2 | - |
Proteobacteria S1 | ? | - |
? | |
| 4-chlorophenol + NADPH + O2 | - |
Proteobacteria | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | alpha4, 4 * 64000, SDS-PAGE | Proteobacteria |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | 1 mol of enzyme contains: 4 mol FAD | Proteobacteria | |
| NADH | - |
Proteobacteria | |
| NADPH | - |
Proteobacteria | |
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