Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxybenzoate + NADPH + H+ + O2 | Pseudomonas fluorescens | - |
3,4-dihydroxybenzoate + NADP+ + H2O | - |
ir | |
4-hydroxybenzoate + NADPH + H+ + O2 | Pseudomonas fluorescens Migula 1895 | - |
3,4-dihydroxybenzoate + NADP+ + H2O | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | P00438 | - |
- |
Pseudomonas fluorescens Migula 1895 | P00438 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxybenzoate + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | 3,4-dihydroxybenzoate + NADP+ + H2O | - |
ir | |
4-hydroxybenzoate + NADPH + H+ + O2 | - |
Pseudomonas fluorescens Migula 1895 | 3,4-dihydroxybenzoate + NADP+ + H2O | - |
ir | |
additional information | development of a colorimetric coupled assay: the glucose-6-phosphate dehydrogenase (G6PD) catalyzes the specific dehydrogenation of glucose-6-phosphate (G-6-P) by consuming the oxidized form of beta-nicotinamide adenine dinucleotide phosphate (NADP+) to generate NADPH. And the formed NADPH initiates the irreversible hydroxylation of p-hydroxybenzoate by p-hydroxybenzoate hydroxylase (PHBH) to generate 3,4-dihydroxybenzoate (DHB), which results in the subsequent in situ generation of a photoresponsive nanozyme of DHB coordinated SrTiO3 (SrTiO3/DHB) nanosheets. The photoresponsive nanozyme has intriguing interfacial charge transfer (ICT) characteristics and thus realizes the efficiently catalytic oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) under light irradiation for signal readout. Optimization and evaluation of the ultrasensitive colorimetric bioassay for versatile targets, overview | Pseudomonas fluorescens | ? | - |
- |
|
additional information | development of a colorimetric coupled assay: the glucose-6-phosphate dehydrogenase (G6PD) catalyzes the specific dehydrogenation of glucose-6-phosphate (G-6-P) by consuming the oxidized form of beta-nicotinamide adenine dinucleotide phosphate (NADP+) to generate NADPH. And the formed NADPH initiates the irreversible hydroxylation of p-hydroxybenzoate by p-hydroxybenzoate hydroxylase (PHBH) to generate 3,4-dihydroxybenzoate (DHB), which results in the subsequent in situ generation of a photoresponsive nanozyme of DHB coordinated SrTiO3 (SrTiO3/DHB) nanosheets. The photoresponsive nanozyme has intriguing interfacial charge transfer (ICT) characteristics and thus realizes the efficiently catalytic oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) under light irradiation for signal readout. Optimization and evaluation of the ultrasensitive colorimetric bioassay for versatile targets, overview | Pseudomonas fluorescens Migula 1895 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
p-hydroxybenzoate hydroxylase | - |
Pseudomonas fluorescens |
PHBH | - |
Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Pseudomonas fluorescens | |
NADPH | - |
Pseudomonas fluorescens |