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Literature summary for 1.14.13.2 extracted from

  • Chang, H.K.; Zylstra, G.J.
    Examination and expansion of the substrate range of m-hydroxybenzoate hydroxylase (2008), Biochem. Biophys. Res. Commun., 371, 149-153.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
mobA gene from pSEB2 cloned into pZR80. Random mutations introduced into the mobA gene and cloned into pCR2.1-TOPO and transformed into Escherichia coli Top10 Comamonas testosteroni

Protein Variants

Protein Variants Comment Organism
A16T/S394P/D416A low ability to hydroxylate 3-aminophenol Comamonas testosteroni
A400G transforms 3-aminophenol with efficiency almost like mutant A400G/K429R Comamonas testosteroni
A400G/K429R among mutants, highest enzymatic activity to hydroxylate 3-aminophenol Comamonas testosteroni
H135P alters the enzyme's substrate specificity Comamonas testosteroni
H135P low ability to hydroxylate 3-aminophenol Comamonas testosteroni
H135P/I217L/Y304H low ability to hydroxylate 3-aminophenol Comamonas testosteroni
K326I lacks the ability to transform phenol to catechol as the wild-type Comamonas testosteroni
K429R can not transform 3-aminophenol at all Comamonas testosteroni
N102T/I259S/V399M low ability to hydroxylate 3-aminophenol Comamonas testosteroni
N227H is not able to transform 3-aminophenol Comamonas testosteroni
N227H/D416A almost has the same transformation efficiency as mutant N227H/Q292R/D416A Comamonas testosteroni
N227H/Q292R/D416A among mutants, highest enzymatic activity to hydroxylate 3-aminophenol Comamonas testosteroni
Q292R is not able to transform 3-aminophenol Comamonas testosteroni
R152L/F364V low ability to hydroxylate 3-aminophenol Comamonas testosteroni
V257A mutation enables the mutant to transform phenol to catechol, also has enhanced ability to transform resorcinol, hydroquinone, p-hydroxybenzoate, 2,5-dihydroxybenzoate, 3,4-dihydroxybenzoate, 3-chlorophenol, 4-chlorophenol, 4-chlororesorcinol, and 4-nitrophenol, thus broadens the substrate range. Is not capable of hydroxylating benzoate, o-hydroxybenzoate (salicylate), 2,4-dihydroxybenzoate, 2,6-dihydroxybenzoate, 2-chlorophenol, 3-aminophenol, 4-methoxybenzoate, 3-toluate, o-cresol, m-cresol, or p-cresol as the wild-type Comamonas testosteroni

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni Q6SSJ6
-
-
Comamonas testosteroni GZ39 Q6SSJ6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dihydroxybenzoate + NADPH + O2
-
Comamonas testosteroni ? + NADP+ + H2O
-
?
2,3-dihydroxybenzoate + NADPH + O2
-
Comamonas testosteroni GZ39 ? + NADP+ + H2O
-
?
2,5-dihydroxybenzoate + NADPH + O2
-
Comamonas testosteroni ? + NADP+ + H2O
-
?
2,5-dihydroxybenzoate + NADPH + O2
-
Comamonas testosteroni GZ39 ? + NADP+ + H2O
-
?
3,4-dihydroxybenzoate + NADPH + O2
-
Comamonas testosteroni ? + NADP+ + H2O
-
?
3,4-dihydroxybenzoate + NADPH + O2
-
Comamonas testosteroni GZ39 ? + NADP+ + H2O
-
?
3,5-dihydroxybenzoate + NADPH + O2
-
Comamonas testosteroni ? + NADP+ + H2O
-
?
3,5-dihydroxybenzoate + NADPH + O2
-
Comamonas testosteroni GZ39 ? + NADP+ + H2O
-
?
3-chlorophenol + NADPH + O2
-
Comamonas testosteroni ? + NADP+ + H2O
-
?
3-hydroxyanthranilate + NADPH + O2
-
Comamonas testosteroni ? + NADP+ + H2O
-
?
4-chlorophenol + NADPH + O2
-
Comamonas testosteroni ? + NADP+ + H2O
-
?
4-chlororesorcinol + NADPH + O2
-
Comamonas testosteroni ? + NADP+ + H2O
-
?
4-nitrophenol + NADPH + O2
-
Comamonas testosteroni ? + NADP+ + H2O
-
?
hydroquinone + NADPH + H+ + O2
-
Comamonas testosteroni ? + NADP+ + H2O
-
?
m-hydroxybenzoate + NADPH + O2
-
Comamonas testosteroni protocatechuate + NADP+ + H2O
-
?
additional information is not capable of hydroxylating benzoate, o-hydroxybenzoate (salicylate), 2,4-dihydroxybenzoate, 2,6-dihydroxybenzoate, 2-chlorophenol, 3-aminophenol, 4-methoxybenzoate, 3-toluate, o-cresol, m-cresol, or p-cresol Comamonas testosteroni ?
-
?
additional information is not capable of hydroxylating benzoate, o-hydroxybenzoate (salicylate), 2,4-dihydroxybenzoate, 2,6-dihydroxybenzoate, 2-chlorophenol, 3-aminophenol, 4-methoxybenzoate, 3-toluate, o-cresol, m-cresol, or p-cresol Comamonas testosteroni GZ39 ?
-
?
p-hydroxybenzoate + NADPH + O2 is transformed to a lesser extent than m-hydroxybenzoate Comamonas testosteroni ? + NADP+ + H2O
-
?

Synonyms

Synonyms Comment Organism
m-hydroxybenzoate hydroxylase
-
Comamonas testosteroni
MobA
-
Comamonas testosteroni

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Comamonas testosteroni