Cloned (Comment) | Organism |
---|---|
mobA gene from pSEB2 cloned into pZR80. Random mutations introduced into the mobA gene and cloned into pCR2.1-TOPO and transformed into Escherichia coli Top10 | Comamonas testosteroni |
Protein Variants | Comment | Organism |
---|---|---|
A16T/S394P/D416A | low ability to hydroxylate 3-aminophenol | Comamonas testosteroni |
A400G | transforms 3-aminophenol with efficiency almost like mutant A400G/K429R | Comamonas testosteroni |
A400G/K429R | among mutants, highest enzymatic activity to hydroxylate 3-aminophenol | Comamonas testosteroni |
H135P | alters the enzyme's substrate specificity | Comamonas testosteroni |
H135P | low ability to hydroxylate 3-aminophenol | Comamonas testosteroni |
H135P/I217L/Y304H | low ability to hydroxylate 3-aminophenol | Comamonas testosteroni |
K326I | lacks the ability to transform phenol to catechol as the wild-type | Comamonas testosteroni |
K429R | can not transform 3-aminophenol at all | Comamonas testosteroni |
N102T/I259S/V399M | low ability to hydroxylate 3-aminophenol | Comamonas testosteroni |
N227H | is not able to transform 3-aminophenol | Comamonas testosteroni |
N227H/D416A | almost has the same transformation efficiency as mutant N227H/Q292R/D416A | Comamonas testosteroni |
N227H/Q292R/D416A | among mutants, highest enzymatic activity to hydroxylate 3-aminophenol | Comamonas testosteroni |
Q292R | is not able to transform 3-aminophenol | Comamonas testosteroni |
R152L/F364V | low ability to hydroxylate 3-aminophenol | Comamonas testosteroni |
V257A | mutation enables the mutant to transform phenol to catechol, also has enhanced ability to transform resorcinol, hydroquinone, p-hydroxybenzoate, 2,5-dihydroxybenzoate, 3,4-dihydroxybenzoate, 3-chlorophenol, 4-chlorophenol, 4-chlororesorcinol, and 4-nitrophenol, thus broadens the substrate range. Is not capable of hydroxylating benzoate, o-hydroxybenzoate (salicylate), 2,4-dihydroxybenzoate, 2,6-dihydroxybenzoate, 2-chlorophenol, 3-aminophenol, 4-methoxybenzoate, 3-toluate, o-cresol, m-cresol, or p-cresol as the wild-type | Comamonas testosteroni |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Comamonas testosteroni | Q6SSJ6 | - |
- |
Comamonas testosteroni GZ39 | Q6SSJ6 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-dihydroxybenzoate + NADPH + O2 | - |
Comamonas testosteroni | ? + NADP+ + H2O | - |
? | |
2,3-dihydroxybenzoate + NADPH + O2 | - |
Comamonas testosteroni GZ39 | ? + NADP+ + H2O | - |
? | |
2,5-dihydroxybenzoate + NADPH + O2 | - |
Comamonas testosteroni | ? + NADP+ + H2O | - |
? | |
2,5-dihydroxybenzoate + NADPH + O2 | - |
Comamonas testosteroni GZ39 | ? + NADP+ + H2O | - |
? | |
3,4-dihydroxybenzoate + NADPH + O2 | - |
Comamonas testosteroni | ? + NADP+ + H2O | - |
? | |
3,4-dihydroxybenzoate + NADPH + O2 | - |
Comamonas testosteroni GZ39 | ? + NADP+ + H2O | - |
? | |
3,5-dihydroxybenzoate + NADPH + O2 | - |
Comamonas testosteroni | ? + NADP+ + H2O | - |
? | |
3,5-dihydroxybenzoate + NADPH + O2 | - |
Comamonas testosteroni GZ39 | ? + NADP+ + H2O | - |
? | |
3-chlorophenol + NADPH + O2 | - |
Comamonas testosteroni | ? + NADP+ + H2O | - |
? | |
3-hydroxyanthranilate + NADPH + O2 | - |
Comamonas testosteroni | ? + NADP+ + H2O | - |
? | |
4-chlorophenol + NADPH + O2 | - |
Comamonas testosteroni | ? + NADP+ + H2O | - |
? | |
4-chlororesorcinol + NADPH + O2 | - |
Comamonas testosteroni | ? + NADP+ + H2O | - |
? | |
4-nitrophenol + NADPH + O2 | - |
Comamonas testosteroni | ? + NADP+ + H2O | - |
? | |
hydroquinone + NADPH + H+ + O2 | - |
Comamonas testosteroni | ? + NADP+ + H2O | - |
? | |
m-hydroxybenzoate + NADPH + O2 | - |
Comamonas testosteroni | protocatechuate + NADP+ + H2O | - |
? | |
additional information | is not capable of hydroxylating benzoate, o-hydroxybenzoate (salicylate), 2,4-dihydroxybenzoate, 2,6-dihydroxybenzoate, 2-chlorophenol, 3-aminophenol, 4-methoxybenzoate, 3-toluate, o-cresol, m-cresol, or p-cresol | Comamonas testosteroni | ? | - |
? | |
additional information | is not capable of hydroxylating benzoate, o-hydroxybenzoate (salicylate), 2,4-dihydroxybenzoate, 2,6-dihydroxybenzoate, 2-chlorophenol, 3-aminophenol, 4-methoxybenzoate, 3-toluate, o-cresol, m-cresol, or p-cresol | Comamonas testosteroni GZ39 | ? | - |
? | |
p-hydroxybenzoate + NADPH + O2 | is transformed to a lesser extent than m-hydroxybenzoate | Comamonas testosteroni | ? + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
m-hydroxybenzoate hydroxylase | - |
Comamonas testosteroni |
MobA | - |
Comamonas testosteroni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Comamonas testosteroni |