Crystallization (Comment) | Organism |
---|---|
- |
Pseudomonas fluorescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-hydroxybenzoate | above 1 mM | Pseudomonas fluorescens | |
4-Aminobenzoate | above 1 mM | Pseudomonas fluorescens | |
4-Fluorobenzoate | above 1 mM | Pseudomonas fluorescens | |
4-hydroxybenzoate | above 1 mM | Pseudomonas fluorescens | |
benzoate | above 1 mM | Pseudomonas fluorescens | |
Fe2+ | - |
Pseudomonas fluorescens | |
protocatechuate | above 1 mM | Pseudomonas fluorescens | |
salicylate | above 1 mM | Pseudomonas fluorescens | |
Zn2+ | 0.2 mM, reversed by 10 mM glutathione | Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0213 | - |
4-hydroxybenzoate | - |
Pseudomonas fluorescens | |
0.0227 | - |
NADPH | reaction with 4-hydroxybenzoate | Pseudomonas fluorescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | - |
gel filtration | Pseudomonas fluorescens |
83600 | - |
low speed sedimentation without reaching equilibrium | Pseudomonas fluorescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxybenzoate + NADPH + O2 | Pseudomonas fluorescens | inducible enzyme | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dihydroxybenzoate + NADPH + O2 | 1.5% of the activity with 4-hydroxybenzoate | Pseudomonas fluorescens | 2,3,4-trihydroxybenzoate + 2,4,5-trihydroxybenzoate + NADP+ + H2O | - |
? | |
3-bromo-4-hydroxybenzoate + NADPH + O2 | 3.2% of the activity with 4-hydroxybenzoate | Pseudomonas fluorescens | ? | - |
? | |
4-hydroxybenzoate + NADPH + O2 | - |
Pseudomonas fluorescens | protocatechuate + NADP+ + H2O | - |
? | |
4-hydroxybenzoate + NADPH + O2 | inducible enzyme | Pseudomonas fluorescens | ? | - |
? | |
4-toluate + NADPH + O2 | 0.29% of the activity with 4-hydroxybenzoate | Pseudomonas fluorescens | ? | - |
? | |
benzene sulfonate + NADPH + O2 | 0.34% of the activity with 4-hydroxybenzoate | Pseudomonas fluorescens | ? | - |
? | |
additional information | under anaerobic conditions, the enzyme can catalyze a reduction of FAD by NADPH provided that 4-hydroxybenzoate is present | Pseudomonas fluorescens | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
36.7 | - |
4-hydroxybenzoate | - |
Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains approximately 0.8 mol FAD per mol of enzyme | Pseudomonas fluorescens | |
NADPH | no activity with NADH | Pseudomonas fluorescens |