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Literature summary for 1.14.13.195 extracted from
- Consecutive enzymatic modification of ornithine generates the hydroxamate moieties of the siderophore erythrochelin (2011), Biochemistry, 50, 6073-6080.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression as His6-tagged protein in Escherichia coli | Saccharopolyspora erythraea |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.286 | - |
L-ornithine | pH 8.0, 25°C | Saccharopolyspora erythraea |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-ornithine + NADPH + H+ + O2 | Saccharopolyspora erythraea | the enzyme is involved in biosynthesis of N5-acetyl-N5-hydroxy-L-ornithine, a building block of the hydroxamate-type siderophore erythrochelin | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharopolyspora erythraea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharopolyspora erythraea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-ornithine + NADPH + H+ + O2 | - |
Saccharopolyspora erythraea | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
| L-ornithine + NADPH + H+ + O2 | the enzyme is involved in biosynthesis of N5-acetyl-N5-hydroxy-L-ornithine, a building block of the hydroxamate-type siderophore erythrochelin | Saccharopolyspora erythraea | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| monooxygenase EtcB | - |
Saccharopolyspora erythraea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Saccharopolyspora erythraea |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.327 | - |
L-ornithine | pH 8.0, 25°C | Saccharopolyspora erythraea | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Saccharopolyspora erythraea |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | FAD-dependent enzyme | Saccharopolyspora erythraea | |
| NADPH | no formation of N5-hydroxy-L-ornithine when NADH is utilized instead of NADPH as the reducing cosubstrate | Saccharopolyspora erythraea | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.14 | - |
L-ornithine | pH 8.0, 25°C | Saccharopolyspora erythraea | |
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Release 2023.1 (January 2023)
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