Cloned (Comment) | Organism |
---|---|
gene cchB, overexpression of N-terminally thioredoxin-tagged enzyme in Escherichia coli strain BL21(DE3) | Streptomyces coelicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Streptomyces coelicolor |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine + NADPH + H+ + O2 | Streptomyces coelicolor | - |
N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
L-ornithine + NADPH + H+ + O2 | Streptomyces coelicolor A3(2) | - |
N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | - |
gene cchB | - |
Streptomyces coelicolor A3(2) | - |
gene cchB | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
flavoprotein | - |
Streptomyces coelicolor |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally thioredoxin-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine + NADPH + H+ + O2 | - |
Streptomyces coelicolor | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
L-ornithine + NADPH + H+ + O2 | absolutely specific for L-ornithine | Streptomyces coelicolor | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
L-ornithine + NADPH + H+ + O2 | - |
Streptomyces coelicolor A3(2) | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
L-ornithine + NADPH + H+ + O2 | absolutely specific for L-ornithine | Streptomyces coelicolor A3(2) | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
additional information | no activity with D-ornithine, N5-formylornithine, L-lysine, L-glutamate, L-glutamine, L-valine, and the tetrapeptide D-Orn-D-Thr-L-Orn-D-Orn, the enzyme performs NADPH oxidation with formation of H2O2, substrate specificity, overview | Streptomyces coelicolor | ? | - |
? | |
additional information | no activity with D-ornithine, N5-formylornithine, L-lysine, L-glutamate, L-glutamine, L-valine, and the tetrapeptide D-Orn-D-Thr-L-Orn-D-Orn, the enzyme performs NADPH oxidation with formation of H2O2, substrate specificity, overview | Streptomyces coelicolor A3(2) | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CchB | - |
Streptomyces coelicolor |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Streptomyces coelicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Streptomyces coelicolor |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | activity range, pH profile | Streptomyces coelicolor |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Streptomyces coelicolor | |
NADPH | - |
Streptomyces coelicolor |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is required for synthesis of the nonproteinogenic amino acids N5-hydroxyornithine and N5-hydroxyformylornithine, that are required for iron assembly by the organism | Streptomyces coelicolor |