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Literature summary for 1.14.13.195 extracted from
- Heterologous expression, purification, and characterization of an L-ornithine N5-hydroxylase involved in pyoverdine siderophore biosynthesis in Pseudomonas aeruginosa (2006), J. Bacteriol., 188, 7205-7210.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| L-lysine | nonsubstrate activator stimulating NADPh oxidation 3.9fold at 2 mM causing H2O2 formation, overview | Pseudomonas aeruginosa |  |
| L-ornithine | activates 5fold | Pseudomonas aeruginosa | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene pvdA, expression of His-tagged enzyme in Escherichia coli strain Rosetta2(DE3) | Pseudomonas aeruginosa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-aminopentanoic acid | competitive inhibition | Pseudomonas aeruginosa | |
| L-2,4-diaminobutyrate | competitive inhibition | Pseudomonas aeruginosa | |
| L-homoserine | competitive inhibition | Pseudomonas aeruginosa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten steady-state kinetic analysis, overview | Pseudomonas aeruginosa | |
| 0.0219 | - |
FAD | pH 8.0, 25°C, recombinant detagged enzyme | Pseudomonas aeruginosa | |
| 0.058 | - |
L-ornithine | pH 8.0, 25°C, recombinant detagged enzyme | Pseudomonas aeruginosa | |
| 0.16 | - |
NADPH | pH 8.0, 25°C, recombinant detagged enzyme | Pseudomonas aeruginosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51000 | - |
x * 51000, recombinant His-tagged enzyme, SDS-PAGE | Pseudomonas aeruginosa |
| 136600 | - |
recombinant His-tagged enzyme, gel filtration | Pseudomonas aeruginosa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-ornithine + NADPH + H+ + O2 | Pseudomonas aeruginosa | - |
N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
gene pvdA | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| flavoprotein | - |
Pseudomonas aeruginosa |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain Rosetta2(DE3) by nickel affinity chromatography, the tag is cleaved off by thrombin | Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.24 | - |
NADPH oxidase activity | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-ornithine + NADPH + H+ + O2 | - |
Pseudomonas aeruginosa | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
| L-ornithine + NADPH + H+ + O2 | no activity with D-ornithine | Pseudomonas aeruginosa | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
| additional information | the NADPH oxidase activity of the enzyme is tightly coupled to hydroxylamine formation | Pseudomonas aeruginosa | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer or pentamer | x * 51000, recombinant His-tagged enzyme, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L-ornithine N5-hydroxylase | - |
Pseudomonas aeruginosa |
| PvdA | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Pseudomonas aeruginosa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | pH profile, overview | Pseudomonas aeruginosa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | specific for | Pseudomonas aeruginosa | |
| additional information | no activity with FMN and NADH | Pseudomonas aeruginosa | |
| NADPH | specific for | Pseudomonas aeruginosa | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | inhibition kinetics | Pseudomonas aeruginosa | |
| 2.9 | - |
5-aminopentanoic acid | pH 8.0, 25°C, recombinant detagged enzyme | Pseudomonas aeruginosa | |
| 3.1 | - |
L-homoserine | pH 8.0, 25°C, recombinant detagged enzyme | Pseudomonas aeruginosa | |
| 8.4 | - |
L-2,4-diaminobutyrate | pH 8.0, 25°C, recombinant detagged enzyme | Pseudomonas aeruginosa | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is involved in pyoverdine siderophore biosynthesis, pyoverdine is required for acquiration of the essential iron from the host | Pseudomonas aeruginosa |
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