BRENDA - Enzyme Database
show all sequences of 1.14.13.187

MD and QM/MM study on catalytic mechanism of a FAD-dependent enzyme ORF36: For nitro sugar biosynthesis

Li, Y.; Ding, L.; Zhang, Q.; Wang, W.; J. Mol. Graph. Model. 44, 9-16 (2013)

Data extracted from this reference:

Organism
Organism
UniProt
Commentary
Textmining
Micromonospora sp.
B5APQ9
-
-
Micromonospora sp. ATCC 39149
B5APQ9
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
TDP-L-epi-vancosamine + 2 NADPH + 2 H+ + 2 O2
enzyme catalyzes a two-step-oxidation. For the second oxidation step, three elementary catalytic steps are found, i.e. a hydroxylation step, a hydrogen back-transfer step and a hydroxyl group elimination step. The hydroxylation step is the rate-determining step with an energy barrier of 26.3 kcal/mol. An oxygen atom from the coenzyme FADHOOH is inserted into the product. Groups Gly132/Ala133/Leu134, Met375/Gln376 and a water fence play a key role in facilitating the rate-determining step. Residues Leu160, Val161 and Ser162 are critical to suppress the rate-determining step
728170
Micromonospora sp.
? + 2 NADP+ + 3 H2O
-
-
-
?
TDP-L-epi-vancosamine + 2 NADPH + 2 H+ + 2 O2
enzyme catalyzes a two-step-oxidation. For the second oxidation step, three elementary catalytic steps are found, i.e. a hydroxylation step, a hydrogen back-transfer step and a hydroxyl group elimination step. The hydroxylation step is the rate-determining step with an energy barrier of 26.3 kcal/mol. An oxygen atom from the coenzyme FADHOOH is inserted into the product. Groups Gly132/Ala133/Leu134, Met375/Gln376 and a water fence play a key role in facilitating the rate-determining step. Residues Leu160, Val161 and Ser162 are critical to suppress the rate-determining step
728170
Micromonospora sp. ATCC 39149
? + 2 NADP+ + 3 H2O
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
nitrososynthase
-
Micromonospora sp.
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
residues L160 ans F374 are involved in binding
Micromonospora sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
residues L160 ans F374 are involved in binding
Micromonospora sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
TDP-L-epi-vancosamine + 2 NADPH + 2 H+ + 2 O2
enzyme catalyzes a two-step-oxidation. For the second oxidation step, three elementary catalytic steps are found, i.e. a hydroxylation step, a hydrogen back-transfer step and a hydroxyl group elimination step. The hydroxylation step is the rate-determining step with an energy barrier of 26.3 kcal/mol. An oxygen atom from the coenzyme FADHOOH is inserted into the product. Groups Gly132/Ala133/Leu134, Met375/Gln376 and a water fence play a key role in facilitating the rate-determining step. Residues Leu160, Val161 and Ser162 are critical to suppress the rate-determining step
728170
Micromonospora sp.
? + 2 NADP+ + 3 H2O
-
-
-
?
TDP-L-epi-vancosamine + 2 NADPH + 2 H+ + 2 O2
enzyme catalyzes a two-step-oxidation. For the second oxidation step, three elementary catalytic steps are found, i.e. a hydroxylation step, a hydrogen back-transfer step and a hydroxyl group elimination step. The hydroxylation step is the rate-determining step with an energy barrier of 26.3 kcal/mol. An oxygen atom from the coenzyme FADHOOH is inserted into the product. Groups Gly132/Ala133/Leu134, Met375/Gln376 and a water fence play a key role in facilitating the rate-determining step. Residues Leu160, Val161 and Ser162 are critical to suppress the rate-determining step
728170
Micromonospora sp. ATCC 39149
? + 2 NADP+ + 3 H2O
-
-
-
?
Other publictions for EC 1.14.13.187
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743958
Sartor
Structure of DnmZ, a nitrosos ...
Streptomyces peucetius
Acta Crystallogr. Sect. F
71
1205-1214
2015
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727686
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Streptomyces peucetius
J. Am. Chem. Soc.
135
11457-11460
2013
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728170
Li
MD and QM/MM study on catalyti ...
Micromonospora sp., Micromonospora sp. ATCC 39149
J. Mol. Graph. Model.
44
9-16
2013
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726951
Vey
Structure and mechanism of ORF ...
Micromonospora carbonacea, Micromonospora carbonacea ATCC 39149
Biochemistry
49
9306-9317
2010
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725191
Hu
A unifying nitrososynthase inv ...
Micromonospora carbonacea, Micromonospora carbonacea var. africana
J. Am. Chem. Soc.
130
15756-15757
2008
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