Any feedback?
Literature summary for 1.14.13.180 extracted from
- Production of carminic acid by metabolically engineered Escherichia coli (2021), J. Am. Chem. Soc., 143, 5364-5377 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | biosynthesis of carminic acid from glucose in engineered Escherichia coli. Expression of an optimized type II polyketide synthase machinery from Photorhabdus luminescens enables a high-level production of flavokermesic acid upon coexpression of the cyclases ZhuI and ZhuJ from Streptomyces sp. R1128. Mutants of aklavinone 12-hydroxylase (DnrF) from Streptomyces peucetius and C-glucosyltransferase (GtCGT) from Gentiana triflora with 2-5fold enhanced conversion efficiencies can perform hydroxylation and C-glucosylation of flavokermesic acid, respectively, resulting in 0.63 mg/l of carminic acid production from glucose | Streptomyces peucetius |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| homology modeling of structure using RdmE from Streptomyces purpurascens, PDB ID 3IHG | Streptomyces peucetius |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| M56A | 2fold increase in synthesis of kermesic acid | Streptomyces peucetius |
| P217K | 2.2fold increase in synthesis of kermesic acid | Streptomyces peucetius |
| P217K/M56A | significant decrease in synthesis of kermesic acid | Streptomyces peucetius |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0433 | - |
flavokermesic acid | mutant P217K, pH not specified in the publication, temperature not specified in the publication | Streptomyces peucetius | |
| 0.0568 | - |
flavokermesic acid | wild-type, pH not specified in the publication, temperature not specified in the publication | Streptomyces peucetius |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces peucetius | P32009 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| C-glucosyl flavokermesic acid + NADPH + H+ + O2 | - |
Streptomyces peucetius | carminic acid + NADP+ + H2O | - |
? |  |
| flavokermesic acid + NADPH + H+ + O2 | - |
Streptomyces peucetius | kermesic acid + NADP+ + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| aklavinone 12-hydroxylase | - |
Streptomyces peucetius |
| DnrF | - |
Streptomyces peucetius |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
