Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.13.180 extracted from

  • Niemi, J.; Wang, Y.; Airas, K.; Ylihonko, K.; Hakala, J.; Mäntsälä, P.
    Characterization of aklavinone-11-hydroxylase from Streptomyces purpurascens (1999), Biochim. Biophys. Acta, 1430, 57-64.
    View publication on PubMed
Search for more literature for 1.14.13.180

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of the gene in Streptomyces lividans Streptomyces purpurascens

Inhibitors

Inhibitors Comment Organism Structure
2,3-Butanedione
-
 Streptomyces purpurascens
Phenylglyoxal NADPH protects against inactivation of aklavinone-11-hydroxylase by phenylglyoxal Streptomyces purpurascens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 aklavinone pH and temperature not specified in the publication Streptomyces purpurascens
2
-
 NADPH pH and temperature not specified in the publication Streptomyces purpurascens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 1 * 60000 Streptomyces purpurascens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
aklavinone + NADPH + H+ + O2 Streptomyces purpurascens
-
 epsilon-rhodomycinone + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces purpurascens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aklavinone + NADH + H+ + O2 the enzyme participates in the biosynthesis of daunorubicin, doxorubicin and rhodomycins Streptomyces purpurascens epsilon-rhodomycinone + NAD+ + H2O
-
 ?
aklavinone + NADPH + H+ + O2
-
 Streptomyces purpurascens epsilon-rhodomycinone + NADP+ + H2O
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 60000 Streptomyces purpurascens

Synonyms

Synonyms Comment Organism
RdmE
-
 Streptomyces purpurascens

Cofactor

Cofactor Comment Organism Structure
FAD the enzyme is a FAD monooxygenase Streptomyces purpurascens
NADH aklavinone-11-hydroxylase can use both NADH and NADPH as coenzyme but it is slowly inactivated in the presence of NADH Streptomyces purpurascens
NADPH aklavinone-11-hydroxylase can use both NADH and NADPH as coenzyme but it is slowly inactivated in the presence of NADH Streptomyces purpurascens

General Information

General Information Comment Organism
physiological function the enzyme participates in the biosynthesis of daunorubicin, doxorubicin and rhodomycins Streptomyces purpurascens