BRENDA - Enzyme Database
show all sequences of 1.14.13.178

Characterization of a broad-specificity non-haem iron N-demethylase from Pseudomonas putida CBB5 capable of utilizing several purine alkaloids as sole carbon and nitrogen source

Summers, R.M.; Louie, T.M.; Yu, C.L.; Subramanian, M.; Microbiology 157, 583-592 (2011)

Data extracted from this reference:

Metals/Ions
Metals/Ions
Commentary
Organism
Structure
non-heme iron
a non-heme iron oxygenase
Pseudomonas putida
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas putida
H9N289
-
-
Pseudomonas putida CBB5
H9N289
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Pseudomonas putida
Reaction
Reaction
Commentary
Organism
Reaction ID
caffeine + O2 + NAD(P)H + H+ = theobromine + NAD(P)+ + H2O + formaldehyde
(1)
Pseudomonas putida
paraxanthine + O2 + NAD(P)H + H+ = 7-methylxanthine + NAD(P)+ + H2O + formaldehyde
(3)
Pseudomonas putida
theophylline + O2 + NAD(P)H + H+ = 3-methylxanthine + NAD(P)+ + H2O + formaldehyde
(2)
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
caffeine + O2 + NAD(P)H + H+
-
709854
Pseudomonas putida
theobromine + NAD(P)+ + H2O + formaldehyde
-
-
-
?
caffeine + O2 + NAD(P)H + H+
-
709854
Pseudomonas putida CBB5
theobromine + NAD(P)+ + H2O + formaldehyde
-
-
-
?
paraxanthine + O2 + NAD(P)H + H+
-
709854
Pseudomonas putida
7-methylxanthine + NAD(P)+ + H2O + formaldehyde
-
-
-
?
paraxanthine + O2 + NAD(P)H + H+
-
709854
Pseudomonas putida CBB5
7-methylxanthine + NAD(P)+ + H2O + formaldehyde
-
-
-
?
theophylline + O2 + NAD(P)H + H+
-
709854
Pseudomonas putida
3-methylxanthine + NAD(P)+ + H2O + formaldehyde
-
-
-
?
theophylline + O2 + NAD(P)H + H+
-
709854
Pseudomonas putida CBB5
3-methylxanthine + NAD(P)+ + H2O + formaldehyde
-
-
-
?
Subunits
Subunits
Commentary
Organism
More
the soluble N-demethylase holoenzyme is composed of two components, a reductase component with cytochrome c reductase activity (Ccr) and a two-subunit N-demethylase component (Ndm). Ndm, with a native molecular mass of 240000 Da, is composed of NdmA (40000 Da) and NdmB (35000 Da). Ccr transfers reducing equivalents from NAD(P)H to Ndm, which catalyses an oxygen-dependent N-demethylation of methylxanthines to xanthine, formaldehyde and water
Pseudomonas putida
Synonyms
Synonyms
Commentary
Organism
ndmA
-
Pseudomonas putida
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Pseudomonas putida
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Pseudomonas putida
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
a reductase component with cytochrome c reductase activity transfers reducing equivalents from NAD(P)H to the enzyme. NADH is the preferred substrate of the reductase component. Activity with NADPH is 22% of that with NADH
Pseudomonas putida
NADPH
a reductase component with cytochrome c reductase activity transfers reducing equivalents from NAD(P)H to the enzyme. NADH is the preferred substrate of the reductase component. Activity with NADPH is 22% of that with NADH
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
a reductase component with cytochrome c reductase activity transfers reducing equivalents from NAD(P)H to the enzyme. NADH is the preferred substrate of the reductase component. Activity with NADPH is 22% of that with NADH
Pseudomonas putida
NADPH
a reductase component with cytochrome c reductase activity transfers reducing equivalents from NAD(P)H to the enzyme. NADH is the preferred substrate of the reductase component. Activity with NADPH is 22% of that with NADH
Pseudomonas putida
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
non-heme iron
a non-heme iron oxygenase
Pseudomonas putida
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
caffeine + O2 + NAD(P)H + H+
-
709854
Pseudomonas putida
theobromine + NAD(P)+ + H2O + formaldehyde
-
-
-
?
caffeine + O2 + NAD(P)H + H+
-
709854
Pseudomonas putida CBB5
theobromine + NAD(P)+ + H2O + formaldehyde
-
-
-
?
paraxanthine + O2 + NAD(P)H + H+
-
709854
Pseudomonas putida
7-methylxanthine + NAD(P)+ + H2O + formaldehyde
-
-
-
?
paraxanthine + O2 + NAD(P)H + H+
-
709854
Pseudomonas putida CBB5
7-methylxanthine + NAD(P)+ + H2O + formaldehyde
-
-
-
?
theophylline + O2 + NAD(P)H + H+
-
709854
Pseudomonas putida
3-methylxanthine + NAD(P)+ + H2O + formaldehyde
-
-
-
?
theophylline + O2 + NAD(P)H + H+
-
709854
Pseudomonas putida CBB5
3-methylxanthine + NAD(P)+ + H2O + formaldehyde
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
the soluble N-demethylase holoenzyme is composed of two components, a reductase component with cytochrome c reductase activity (Ccr) and a two-subunit N-demethylase component (Ndm). Ndm, with a native molecular mass of 240000 Da, is composed of NdmA (40000 Da) and NdmB (35000 Da). Ccr transfers reducing equivalents from NAD(P)H to Ndm, which catalyses an oxygen-dependent N-demethylation of methylxanthines to xanthine, formaldehyde and water
Pseudomonas putida
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Pseudomonas putida
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Pseudomonas putida
Other publictions for EC 1.14.13.178
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
722538
Summers
Novel, highly specific N-demet ...
Pseudomonas putida, Pseudomonas putida CBB5
J. Bacteriol.
194
2041-2049
2012
-
-
1
-
-
-
-
4
-
1
2
5
-
4
-
-
1
-
-
-
-
-
13
1
1
1
-
-
4
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
4
-
1
2
5
-
-
-
1
-
-
-
-
13
1
1
-
-
4
1
-
-
-
-
1
1
-
4
4
709854
Summers
Characterization of a broad-sp ...
Pseudomonas putida, Pseudomonas putida CBB5
Microbiology
157
583-592
2011
-
-
-
-
-
-
-
-
-
1
-
-
-
4
-
-
1
3
-
-
-
-
6
1
1
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
6
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
724824
Sarkar
Characterization of human live ...
Homo sapiens
Drug Metab. Dispos.
20
31-37
1992
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-
-
-
-
-
-
-
1
-
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1
-
1
-
-
-
-
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1
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1
-
1
-
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-
-
-
-
-
1
-
-
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-
-
-
1
-
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-
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-
-
-
1
-
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1
-
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1
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1
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