Literature summary for 1.14.13.172 extracted from

Sheng, H.; Jing, Y.; An, N.; Shen, X.; Sun, X.; Yan, Y.; Wang, J.; Yuan, Q.
Extending the shikimate pathway for microbial production of maleate from glycerol in engineered Escherichia coli (2021), Biotechnol. Bioeng., 118, 1840-1850 .

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Application

Application	Comment	Organism
synthesis	biosynthetic pathway for maleate production in engineered Escherichia coli. Expression of salicylate 5-hydroxylase, introduction of salicylate biosynthetic pathway and gentisate ring cleavage pathway allow the synthesis of maleate from glycerol. Further optimizations boost maleate titer to 2.4 g/l in shake flask experiments. Subsequent scale-up biosynthesis of maleate in a 3-l bioreactor under fed-batch culture conditions enables the production of 14.5 g/l of maleate	Ralstonia sp.

Organism

Organism	UniProt	Comment	Textmining
Ralstonia sp.	O52379 and O52380	O52379 i.e. large oxygenase component NagG, O52380 i.e. small oxygenase component NagH	-

Synonyms

Synonyms	Comment	Organism
nagG	-	Ralstonia sp.
nagH	-	Ralstonia sp.

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Release 2023.1 (January 2023)