Literature summary for 1.14.13.172 extracted from

Hou, Y.J.; Guo, Y.; Li, D.F.; Zhou, N.Y.
Structural and biochemical analysis reveals a distinct catalytic site of salicylate 5-monooxygenase NagGH from Rieske dioxygenases (2021), Appl. Environ. Microbiol., 87, e01629 .

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Crystallization (Commentary)

Crystallization (Comment)	Organism
structure of the oxygenase component NagGH. The large subunit NagG and small subunit NagH share the same fold as that for their counterparts of Rieske dioxygenases and assemble the same alpha3beta3 hexamer. A potential substrate-binding pocket is observed in the vicinity of the nonheme iron site. Its positively charged residue Arg323 is surrounded by hydrophobic residues. The shift of nonheme iron atom caused by residue Leu228 disrupts the usual substrate pocket observed in other Rieske oxygenases. Residue Asn218 is involved in substrate binding and oxidation, but residues Gln316 and Ser367 play a more important role in substrate oxidation than Asn218	Ralstonia sp.

Crystallization (Comment)

Organism

structure of the oxygenase component NagGH. The large subunit NagG and small subunit NagH share the same fold as that for their counterparts of Rieske dioxygenases and assemble the same alpha3beta3 hexamer. A potential substrate-binding pocket is observed in the vicinity of the nonheme iron site. Its positively charged residue Arg323 is surrounded by hydrophobic residues. The shift of nonheme iron atom caused by residue Leu228 disrupts the usual substrate pocket observed in other Rieske oxygenases. Residue Asn218 is involved in substrate binding and oxidation, but residues Gln316 and Ser367 play a more important role in substrate oxidation than Asn218

Ralstonia sp.

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Iron	nonheme iron site	Ralstonia sp.

Organism

Organism	UniProt	Comment	Textmining
Ralstonia sp.	O52378 and O52381 and O52379 and O52380	O52378 i.e. reductase component NagAa, O52381 i.e. ferredoxin component NagAb, O52379 i.e. large oxygenase component NagH, O52380 i.e. small oxygenase component NagH	-

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Release 2023.1 (January 2023)