Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas putida |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 20 mM HEPES pH 7.0, 15% (w/v) PEG 4000 and 15% (v/v) 2-propanol | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46600 | - |
x * 46600, calculated from amino acid sequence | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | C6FI48 | - |
- |
Pseudomonas putida DLL-E4 | C6FI48 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography and Sephadex G-100 gel filtration | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenol + NADH + H+ + O2 | - |
Pseudomonas putida | 1,4-benzoquinone + nitrite + NAD+ + H2O | - |
? | |
4-nitrophenol + NADH + H+ + O2 | - |
Pseudomonas putida DLL-E4 | 1,4-benzoquinone + nitrite + NAD+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46600, calculated from amino acid sequence | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
para-nitrophenol 4-monooxygenase | - |
Pseudomonas putida |
PNP 4-monooxygenase | - |
Pseudomonas putida |
pnpA | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Pseudomonas putida | |
NADH | - |
Pseudomonas putida |