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Literature summary for 1.14.13.167 extracted from

  • Liu, W.; Shen, W.; Zhao, X.; Cao, H.; Cui, Z.
    Expression, purification, crystallization and preliminary X-ray analysis of para-nitrophenol 4-monooxygenase from Pseudomonas putida DLL-E4 (2009), Acta Crystallogr. Sect. F, 65, 1004-1006.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudomonas putida

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 20 mM HEPES pH 7.0, 15% (w/v) PEG 4000 and 15% (v/v) 2-propanol Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46600
-
x * 46600, calculated from amino acid sequence Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida C6FI48
-
-
Pseudomonas putida DLL-E4 C6FI48
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography and Sephadex G-100 gel filtration Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenol + NADH + H+ + O2
-
Pseudomonas putida 1,4-benzoquinone + nitrite + NAD+ + H2O
-
?
4-nitrophenol + NADH + H+ + O2
-
Pseudomonas putida DLL-E4 1,4-benzoquinone + nitrite + NAD+ + H2O
-
?

Subunits

Subunits Comment Organism
? x * 46600, calculated from amino acid sequence Pseudomonas putida

Synonyms

Synonyms Comment Organism
para-nitrophenol 4-monooxygenase
-
Pseudomonas putida
PNP 4-monooxygenase
-
Pseudomonas putida
pnpA
-
Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
FAD
-
Pseudomonas putida
NADH
-
Pseudomonas putida