BRENDA - Enzyme Database
show all sequences of 1.14.13.166

A two-component monooxygenase catalyzes both the hydroxylation of p-nitrophenol and the oxidative release of nitrite from 4-nitrocatechol in Bacillus sphaericus JS905

Kadiyala, V.; Spain, J.C.; Appl. Environ. Microbiol. 64, 2479-2484 (1998)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
-
Lysinibacillus sphaericus
Methimazole
58% inhibition at 0.5 mM
Lysinibacillus sphaericus
additional information
alpha-naphthoflavone, miconazole, and metyrapone at 0.5 mM have very little effect (less than 5% inhibition) on enzyme activity
Lysinibacillus sphaericus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
magnesium ions enhance the enzyme activity
Lysinibacillus sphaericus
Organism
Organism
UniProt
Commentary
Textmining
Lysinibacillus sphaericus
-
-
-
Lysinibacillus sphaericus JS905
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
DEAE-Sepharose column chromatography, Q-Sepharose column chromatography, and Sephacryl S-300 gel filtration
Lysinibacillus sphaericus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture condition:4-nitrophenol-grown cell
-
Lysinibacillus sphaericus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0013
-
cell extract, using 4-nitrocatechol as substrate, at pH 8.0 and 30°C
Lysinibacillus sphaericus
0.0013
-
cell extract, using 4-nitrophenol as substrate, at pH 8.0 and 30°C
Lysinibacillus sphaericus
0.038
-
after 28fold purification, using 4-nitrocatechol as substrate, at pH 8.0 and 30°C
Lysinibacillus sphaericus
0.04
-
after 32fold purification, using 4-nitrophenol as substrate, at pH 8.0 and 30°C
Lysinibacillus sphaericus
Storage Stability
Storage Stability
Organism
.
Lysinibacillus sphaericus
0°C, extracts prepared without added FAD, 48 h, 52% loss of activity
Lysinibacillus sphaericus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4-nitrocatechol + NADH + H+ + O2
-
438885
Lysinibacillus sphaericus
2-hydroxy-1,4-benzoquinone + nitrite + NAD+ + H2O
-
-
-
?
additional information
NADPH does not support 4-nitrophenol or 4-nitrocatechol oxidation
438885
Lysinibacillus sphaericus
?
-
-
-
?
additional information
NADPH does not support 4-nitrophenol or 4-nitrocatechol oxidation
438885
Lysinibacillus sphaericus JS905
?
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
PNP monooxygenase
the enzyme system consists of two components that catalyze two consecutive monooxygenation reactions
Lysinibacillus sphaericus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
in 50 mM Tris-HCl
Lysinibacillus sphaericus
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
required for full activity. The reductase component of the enzyme has a loosely bound FAD
Lysinibacillus sphaericus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
required for full activity. The reductase component of the enzyme has a loosely bound FAD
Lysinibacillus sphaericus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
-
Lysinibacillus sphaericus
Methimazole
58% inhibition at 0.5 mM
Lysinibacillus sphaericus
additional information
alpha-naphthoflavone, miconazole, and metyrapone at 0.5 mM have very little effect (less than 5% inhibition) on enzyme activity
Lysinibacillus sphaericus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
magnesium ions enhance the enzyme activity
Lysinibacillus sphaericus
Purification (Commentary) (protein specific)
Commentary
Organism
DEAE-Sepharose column chromatography, Q-Sepharose column chromatography, and Sephacryl S-300 gel filtration
Lysinibacillus sphaericus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture condition:4-nitrophenol-grown cell
-
Lysinibacillus sphaericus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0013
-
cell extract, using 4-nitrocatechol as substrate, at pH 8.0 and 30°C
Lysinibacillus sphaericus
0.0013
-
cell extract, using 4-nitrophenol as substrate, at pH 8.0 and 30°C
Lysinibacillus sphaericus
0.038
-
after 28fold purification, using 4-nitrocatechol as substrate, at pH 8.0 and 30°C
Lysinibacillus sphaericus
0.04
-
after 32fold purification, using 4-nitrophenol as substrate, at pH 8.0 and 30°C
Lysinibacillus sphaericus
Storage Stability (protein specific)
Storage Stability
Organism
.
Lysinibacillus sphaericus
0°C, extracts prepared without added FAD, 48 h, 52% loss of activity
Lysinibacillus sphaericus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4-nitrocatechol + NADH + H+ + O2
-
438885
Lysinibacillus sphaericus
2-hydroxy-1,4-benzoquinone + nitrite + NAD+ + H2O
-
-
-
?
additional information
NADPH does not support 4-nitrophenol or 4-nitrocatechol oxidation
438885
Lysinibacillus sphaericus
?
-
-
-
?
additional information
NADPH does not support 4-nitrophenol or 4-nitrocatechol oxidation
438885
Lysinibacillus sphaericus JS905
?
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
in 50 mM Tris-HCl
Lysinibacillus sphaericus
Other publictions for EC 1.14.13.166
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744109
Min
A two-component para-nitrophe ...
no activity in Rhodococcus imtechensis
Appl. Environ. Microbiol.
82
714-723
2016
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1
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-
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-
-
-
-
-
-
745126
Kallubai
In silico approach to support ...
Arthrobacter sp. JS443, Lysinibacillus sphaericus, Lysinibacillus sphaericus JS905
Interdiscip. Sci. Comput. Life Sci.
7
157-167
2015
-
-
-
-
-
-
-
-
-
-
-
3
-
8
-
-
-
-
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-
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6
-
8
-
-
-
-
-
-
-
4
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-
4
-
-
-
-
-
-
-
-
-
-
3
-
-
-
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-
-
-
6
-
-
-
-
-
-
-
-
-
-
7
7
-
-
-
719179
Zhang
Identification of the para-nit ...
Pseudomonas sp., Pseudomonas sp. 1-7
BMC Microbiol.
12
27
2012
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8
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2
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3
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2
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-
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-
711336
Wei
para-Nitrophenol 4-monooxygena ...
Pseudomonas sp.
Biodegradation
21
915-921
2010
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
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-
-
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-
-
1
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-
1
-
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-
-
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-
-
-
1
-
-
1
-
-
704288
Zhang
Identification and characteriz ...
Pseudomonas sp.
J. Bacteriol.
191
2703-2710
2009
-
-
1
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-
-
-
-
-
1
1
-
-
6
-
-
1
-
-
1
1
3
1
1
3
-
-
-
-
-
-
-
3
-
-
-
-
-
1
3
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-
-
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-
1
1
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-
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-
1
-
1
1
3
1
1
-
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-
-
-
718490
Liu
Expression, purification, crys ...
Pseudomonas putida, Pseudomonas putida DLL-E4
Acta Crystallogr. Sect. F
65
1004-1006
2009
-
-
1
1
-
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-
-
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-
1
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-
5
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-
1
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-
-
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2
1
3
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2
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1
2
1
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1
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1
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2
1
-
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-
-
-
-
-
-
-
-
-
-
-
719711
Perry
Cloning of a gene cluster invo ...
Arthrobacter sp.
J. Bacteriol.
189
7563-7572
2007
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
1
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-
1
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-
15
-
2
-
-
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-
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-
-
2
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-
1
2
-
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-
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1
-
1
-
-
15
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
438817
Leung
The role of the Sphingomonas s ...
Sphingomonas sp., Sphingomonas sp. UG30
FEMS Microbiol. Lett.
173
247-253
1999
-
-
-
-
-
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1
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-
2
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2
1
1
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1
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1
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1
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-
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-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
438885
Kadiyala
A two-component monooxygenase ...
Lysinibacillus sphaericus, Lysinibacillus sphaericus JS905
Appl. Environ. Microbiol.
64
2479-2484
1998
-
-
-
-
-
-
3
-
-
1
-
-
-
3
-
-
1
-
-
1
4
2
3
-
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
1
-
-
-
-
-
1
-
1
4
2
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-