BRENDA - Enzyme Database
show all sequences of 1.14.13.160

Completing the series of BVMOs involved in camphor metabolism of Pseudomonas putida NCIMB 10007 by identification of the two missing genes, their functional expression in E. coli, and biochemical characterization

Kadow, M.; Loschinski, K.; Sass, S.; Schmidt, M.; Bornscheuer, U.; Appl. Microbiol. Biotechnol. 96, 419-429 (2012)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Pseudomonas putida
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.028
-
(+-)-cis-bicyclo[3.2.0]hept-2-en-6-one
pH 9.0, 20°C
Pseudomonas putida
0.127
-
Cyclobutanone
pH 9.0, 20°C
Pseudomonas putida
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
x * 60000, SDS-PAGE, x * 63500, calculated, both for His-tagged protein
Pseudomonas putida
63500
-
x * 60000, SDS-PAGE, x * 63500, calculated, both for His-tagged protein
Pseudomonas putida
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas putida
H3JQW0
-
-
Pseudomonas putida NCIMB 10007
H3JQW0
-
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant enzyme
Pseudomonas putida
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2200
-
pH 9.0, 20°C
Pseudomonas putida
Storage Stability
Storage Stability
Organism
4°C, 24 h, 50% loss of activity
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(+-)-cis-bicyclo[3.2.0]hept-2-en-6-one + O2 + NADPH + H+
-
717141
Pseudomonas putida
? + NADP+ + H2O
-
-
-
-
(+-)-cis-bicyclo[3.2.0]hept-2-en-6-one + O2 + NADPH + H+
-
717141
Pseudomonas putida NCIMB 10007
? + NADP+ + H2O
-
-
-
-
2,3,4,5-tetramethyl-2-cyclopenten-1-one + O2 + NADPH + H+
34% conversion
717141
Pseudomonas putida
? + NADP+ + H2O
-
-
-
-
2,3,4,5-tetramethyl-2-cyclopenten-1-one + O2 + NADPH + H+
34% conversion
717141
Pseudomonas putida NCIMB 10007
? + NADP+ + H2O
-
-
-
-
2-cyclohexen-1-one + O2 + NADPH + H+
74% conversion
717141
Pseudomonas putida
? + NADP+ + H2O
-
-
-
-
2-cyclohexen-1-one + O2 + NADPH + H+
74% conversion
717141
Pseudomonas putida NCIMB 10007
? + NADP+ + H2O
-
-
-
-
2-cyclopenten-1-one + O2 + NADPH + H+
62% conversion
717141
Pseudomonas putida
? + NADP+ + H2O
-
-
-
-
3,5,5-trimethyl-2-cyclohexen-1-one + O2 + NADPH + H+
22% conversion
717141
Pseudomonas putida
? + NADP+ + H2O
-
-
-
-
3,5,5-trimethyl-2-cyclohexen-1-one + O2 + NADPH + H+
22% conversion
717141
Pseudomonas putida NCIMB 10007
? + NADP+ + H2O
-
-
-
-
3-methyl-2-cyclohexen-1-one + O2 + NADPH + H+
13% conversion
717141
Pseudomonas putida
? + NADP+ + H2O
-
-
-
-
3-methyl-2-cyclohexen-1-one + O2 + NADPH + H+
13% conversion
717141
Pseudomonas putida NCIMB 10007
? + NADP+ + H2O
-
-
-
-
3-methyl-2-cyclopenten-1-one + O2 + NADPH + H+
13% conversion
717141
Pseudomonas putida
? + NADP+ + H2O
-
-
-
-
cyclobutanone + O2 + NADPH + H+
-
717141
Pseudomonas putida
? + NADP+ + H2O
-
-
-
-
norcamphor + O2 + NADPH + H+
-
717141
Pseudomonas putida
? + NADP+ + H2O
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 60000, SDS-PAGE, x * 63500, calculated, both for His-tagged protein
Pseudomonas putida
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
23
-
-
Pseudomonas putida
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
20
-
half-life 196 min
Pseudomonas putida
25
-
half-life 43 min
Pseudomonas putida
30
-
half-life 9 min
Pseudomonas putida
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Pseudomonas putida
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Pseudomonas putida
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Pseudomonas putida
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.028
-
(+-)-cis-bicyclo[3.2.0]hept-2-en-6-one
pH 9.0, 20°C
Pseudomonas putida
0.127
-
Cyclobutanone
pH 9.0, 20°C
Pseudomonas putida
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
x * 60000, SDS-PAGE, x * 63500, calculated, both for His-tagged protein
Pseudomonas putida
63500
-
x * 60000, SDS-PAGE, x * 63500, calculated, both for His-tagged protein
Pseudomonas putida
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme
Pseudomonas putida
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2200
-
pH 9.0, 20°C
Pseudomonas putida
Storage Stability (protein specific)
Storage Stability
Organism
4°C, 24 h, 50% loss of activity
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(+-)-cis-bicyclo[3.2.0]hept-2-en-6-one + O2 + NADPH + H+
-
717141
Pseudomonas putida
? + NADP+ + H2O
-
-
-
-
(+-)-cis-bicyclo[3.2.0]hept-2-en-6-one + O2 + NADPH + H+
-
717141
Pseudomonas putida NCIMB 10007
? + NADP+ + H2O
-
-
-
-
2,3,4,5-tetramethyl-2-cyclopenten-1-one + O2 + NADPH + H+
34% conversion
717141
Pseudomonas putida
? + NADP+ + H2O
-
-
-
-
2,3,4,5-tetramethyl-2-cyclopenten-1-one + O2 + NADPH + H+
34% conversion
717141
Pseudomonas putida NCIMB 10007
? + NADP+ + H2O
-
-
-
-
2-cyclohexen-1-one + O2 + NADPH + H+
74% conversion
717141
Pseudomonas putida
? + NADP+ + H2O
-
-
-
-
2-cyclohexen-1-one + O2 + NADPH + H+
74% conversion
717141
Pseudomonas putida NCIMB 10007
? + NADP+ + H2O
-
-
-
-
2-cyclopenten-1-one + O2 + NADPH + H+
62% conversion
717141
Pseudomonas putida
? + NADP+ + H2O
-
-
-
-
3,5,5-trimethyl-2-cyclohexen-1-one + O2 + NADPH + H+
22% conversion
717141
Pseudomonas putida
? + NADP+ + H2O
-
-
-
-
3,5,5-trimethyl-2-cyclohexen-1-one + O2 + NADPH + H+
22% conversion
717141
Pseudomonas putida NCIMB 10007
? + NADP+ + H2O
-
-
-
-
3-methyl-2-cyclohexen-1-one + O2 + NADPH + H+
13% conversion
717141
Pseudomonas putida
? + NADP+ + H2O
-
-
-
-
3-methyl-2-cyclohexen-1-one + O2 + NADPH + H+
13% conversion
717141
Pseudomonas putida NCIMB 10007
? + NADP+ + H2O
-
-
-
-
3-methyl-2-cyclopenten-1-one + O2 + NADPH + H+
13% conversion
717141
Pseudomonas putida
? + NADP+ + H2O
-
-
-
-
cyclobutanone + O2 + NADPH + H+
-
717141
Pseudomonas putida
? + NADP+ + H2O
-
-
-
-
norcamphor + O2 + NADPH + H+
-
717141
Pseudomonas putida
? + NADP+ + H2O
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 60000, SDS-PAGE, x * 63500, calculated, both for His-tagged protein
Pseudomonas putida
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
23
-
-
Pseudomonas putida
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
20
-
half-life 196 min
Pseudomonas putida
25
-
half-life 43 min
Pseudomonas putida
30
-
half-life 9 min
Pseudomonas putida
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Pseudomonas putida
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
14.7
-
Cyclobutanone
pH 9.0, 20°C
Pseudomonas putida
49.3
-
(+-)-cis-bicyclo[3.2.0]hept-2-en-6-one
pH 9.0, 20°C
Pseudomonas putida
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
14.7
-
Cyclobutanone
pH 9.0, 20°C
Pseudomonas putida
49.3
-
(+-)-cis-bicyclo[3.2.0]hept-2-en-6-one
pH 9.0, 20°C
Pseudomonas putida
Other publictions for EC 1.14.13.160
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
717126
Leisch
Cloning, Baeyer-Villiger bioox ...
Pseudomonas putida, Pseudomonas putida ATCC 17453
Appl. Environ. Microbiol.
78
2200-2212
2012
-
-
1
1
-
-
-
6
-
-
3
-
-
4
-
-
1
-
-
-
-
-
15
1
1
-
-
3
8
1
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
6
-
-
3
-
-
-
-
1
-
-
-
-
15
1
-
-
3
8
1
-
-
-
-
-
-
-
6
6
717141
Kadow
Completing the series of BVMOs ...
Pseudomonas putida, Pseudomonas putida NCIMB 10007
Appl. Microbiol. Biotechnol.
96
419-429
2012
-
-
1
-
-
-
-
2
-
-
2
-
-
4
-
-
1
-
-
-
1
1
14
1
-
1
-
3
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
-
2
-
-
-
-
1
-
-
1
1
14
1
1
-
3
-
1
-
-
-
-
-
-
-
2
2
717711
Ougham
Camphor revisited: Involvement ...
no activity in Pseudomonas putida strain PaW340, Pseudomonas putida, Pseudomonas putida C1 / ATCC 17453
J. Bacteriol.
153
140-152
1983
-
-
-
-
1
-
-
-
-
1
2
2
-
10
-
-
1
-
-
1
1
-
8
2
1
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
1
2
2
-
-
-
1
-
1
1
-
8
2
1
-
-
-
1
-
-
-
1
1
1
1
-
-