BRENDA - Enzyme Database show
show all sequences of 1.14.13.16

Purification and properties of cyclopentanone oxygenase of Pseudomonas NCIB 9872

Griffin, M.; Trudgill, P.W.; Eur. J. Biochem. 63, 199-209 (1976)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis(2-nitrobenzoate)
-
Pseudomonas sp.
arsenite
-
Pseudomonas sp.
Bathocuproine
-
Pseudomonas sp.
bathophenanthroline
-
Pseudomonas sp.
diethyldithiocarbamate
-
Pseudomonas sp.
iodoacetamide
-
Pseudomonas sp.
p-hydroxymercuribenzoate
-
Pseudomonas sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.001
-
O2
-
Pseudomonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
200000
-
gel filtration, sedimentation velocity
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
cyclopentanone + NADPH + O2
Pseudomonas sp.
-
5-valerolactone + NADP+ + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
NCIB 9872
-
Oxidation Stability
Oxidation Stability
Organism
reduction of enzyme by visible light in presence of EDTA
Pseudomonas sp.
Purification (Commentary)
Commentary
Organism
-
Pseudomonas sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.3
5
-
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-methylcyclohexanone + NADPH + O2
-
439017
Pseudomonas sp.
1-oxa-2-oxo-3-methylcycloheptane + NADP+ + H2O
-
-
-
?
butan-2-one + NADPH + O2
-
439017
Pseudomonas sp.
?
-
-
-
?
cyclobutanone + NADPH + O2
-
439017
Pseudomonas sp.
butyrolactone + NADP+ + H2O
-
-
-
?
cycloheptanone + NADPH + O2
poor substrate
439017
Pseudomonas sp.
1-oxa-2-oxocyclooctane + NADP+ + H2O
-
-
-
?
cyclohexanone + NADPH + O2
-
439017
Pseudomonas sp.
1-oxa-2-oxocycloheptane + NADP+ + H2O
-
-
-
?
cyclooctanone + NADPH + O2
poor substrate
439017
Pseudomonas sp.
1-oxa-2-oxocyclononane + NADP+ + H2O
-
-
-
?
cyclopentanone + NADPH + O2
-
439017
Pseudomonas sp.
5-valerolactone + NADP+ + H2O
-
-
-
?
norbornanone + NADPH + O2
-
439017
Pseudomonas sp.
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
trimer or tetramer
3 or 4 * 54000-58000, SDS-PAGE
Pseudomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.7
-
-
Pseudomonas sp.
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
FAD : enzyme ratio is 2.2 to 3.5
Pseudomonas sp.
NADPH
-
Pseudomonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
FAD : enzyme ratio is 2.2 to 3.5
Pseudomonas sp.
NADPH
-
Pseudomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis(2-nitrobenzoate)
-
Pseudomonas sp.
arsenite
-
Pseudomonas sp.
Bathocuproine
-
Pseudomonas sp.
bathophenanthroline
-
Pseudomonas sp.
diethyldithiocarbamate
-
Pseudomonas sp.
iodoacetamide
-
Pseudomonas sp.
p-hydroxymercuribenzoate
-
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.001
-
O2
-
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
200000
-
gel filtration, sedimentation velocity
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
cyclopentanone + NADPH + O2
Pseudomonas sp.
-
5-valerolactone + NADP+ + H2O
-
-
?
Oxidation Stability (protein specific)
Oxidation Stability
Organism
reduction of enzyme by visible light in presence of EDTA
Pseudomonas sp.
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.3
5
-
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-methylcyclohexanone + NADPH + O2
-
439017
Pseudomonas sp.
1-oxa-2-oxo-3-methylcycloheptane + NADP+ + H2O
-
-
-
?
butan-2-one + NADPH + O2
-
439017
Pseudomonas sp.
?
-
-
-
?
cyclobutanone + NADPH + O2
-
439017
Pseudomonas sp.
butyrolactone + NADP+ + H2O
-
-
-
?
cycloheptanone + NADPH + O2
poor substrate
439017
Pseudomonas sp.
1-oxa-2-oxocyclooctane + NADP+ + H2O
-
-
-
?
cyclohexanone + NADPH + O2
-
439017
Pseudomonas sp.
1-oxa-2-oxocycloheptane + NADP+ + H2O
-
-
-
?
cyclooctanone + NADPH + O2
poor substrate
439017
Pseudomonas sp.
1-oxa-2-oxocyclononane + NADP+ + H2O
-
-
-
?
cyclopentanone + NADPH + O2
-
439017
Pseudomonas sp.
5-valerolactone + NADP+ + H2O
-
-
-
?
norbornanone + NADPH + O2
-
439017
Pseudomonas sp.
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
trimer or tetramer
3 or 4 * 54000-58000, SDS-PAGE
Pseudomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.7
-
-
Pseudomonas sp.
Other publictions for EC 1.14.13.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743194
Rebros
Biocatalysis with Escherichia ...
Comamonas sp. NCIMB 9872
Lett. Appl. Microbiol.
58
556-563
2014
-
1
1
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-
-
-
-
-
-
-
-
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3
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1
1
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
724608
Schenkmayerova
Microbial monooxygenase ampero ...
Comamonas sp.
Biosens. Bioelectron.
50
235-238
2013
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688437
Clouthier
-
Biotransformations with engine ...
Comamonas sp.
J. Mol. Catal. B
46
32-36
2007
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
673035
Mihovilovic
Accessing tetrahydrofuran-base ...
Comamonas sp.
Chem. Commun. (Camb. )
1
3214-3216
2006
-
1
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
1
1
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
675582
Clouthier
Designing new Baeyer-Villiger ...
Acinetobacter sp., Acinetobacter sp. NCIB 9871
J. Org. Chem.
71
8431-8437
2006
-
-
1
-
1
-
-
-
-
-
-
2
-
6
-
-
-
1
-
-
-
-
10
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
10
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
677179
Clouthier
-
Increasing the enantioselectiv ...
Acinetobacter sp., Acinetobacter sp. NCIB 9871
Tetrahedron Asymmetry
17
2649-2653
2006
-
-
1
-
7
-
-
-
-
-
-
2
-
6
-
-
-
1
-
-
-
-
18
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
7
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659736
Wang
-
Monooxygenase-catalyzed Baeyer ...
Comamonas sp.
J. Mol. Catal. B
22
211-218
2003
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439022
Iwaki
Cloning and characterization o ...
Comamonas sp.
Appl. Environ. Microbiol.
68
5671-5684
2002
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439021
Bes
-
Oxidative biotransformations b ...
Pseudomonas sp.
J. Mol. Catal. , B Enzym.
1
127-134
1996
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
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1
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439020
Trudgill
Cyclopentanone 1,2-monooxygena ...
Pseudomonas sp.
Methods Enzymol.
188
77-81
1990
-
-
-
-
-
-
-
2
-
-
2
1
-
1
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
2
1
-
-
-
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439017
Griffin
Purification and properties of ...
Pseudomonas sp.
Eur. J. Biochem.
63
199-209
1976
-
-
-
-
-
-
7
1
-
-
1
1
-
2
1
-
1
-
-
-
1
-
8
1
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
7
-
1
-
-
1
1
-
1
-
1
-
-
1
-
8
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
439018
Griffin
-
The purification of cyclopenta ...
Pseudomonas sp.
Biochem. Soc. Trans.
1
1255-1258
1973
-
-
-
-
-
-
4
-
-
-
1
1
-
1
-
-
1
-
-
-
1
-
8
1
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
4
-
-
-
-
1
1
-
-
-
1
-
-
1
-
8
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286094
Griffin
The metabolism of cyclopentano ...
Pseudomonas sp.
Biochem. J.
129
595-603
1972
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
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-
1
-
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-
-
-
-
-
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-
-
2
-
-
-
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-
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2
-
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