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Literature summary for 1.14.13.153 extracted from

  • Karp, F.; Harris, J.; Croteau, R.
    Metabolism of monoterpenes: Demonstration of the hydroxylation of (+)-sabinene to (+)-cis-sabinol by an enzyme preparation from sage (Salvia officinalis) leaves (1987), Arch. Biochem. Biophys., 256, 179-193.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
FAD exogenous, activates the enzyme slightly Salvia officinalis
FMN exogenous, activates the enzyme Salvia officinalis
octyl beta-D-glucoside 35% activation at 0.12% detergent, complete inhibition at 0.48% detergent Salvia officinalis
sodium cholate 40% activation at 0.30% detergent, 17% inhibition at 0.48% detergent Salvia officinalis
Zwittergent 3-08 12% activation at 0.24% detergent Salvia officinalis

Inhibitors

Inhibitors Comment Organism Structure
CO photoreversible inhibition Salvia officinalis
NADP+ NADP+ competitively inhibits P-450-dependent reactions, 40-45% inhibition of hydroxylation at 2 mM Salvia officinalis
octyl beta-D-glucoside 35% activation at 0.12% detergent, complete inhibition at 0.48% detergent Salvia officinalis
sodium cholate 40% activation at 0.30% detergent, 17% inhibition at 0.48% detergent Salvia officinalis
sodium deoxycholate 80% inhibition at 0.06% detergent Salvia officinalis
sodium taurocholate 13% inhibition at 0.30% detergent Salvia officinalis
sodium taurodeoxycholate 91% inhibition at 0.06% detergent Salvia officinalis

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome localization of sabinene hydroxylase in sage leaf homogenates by differential centrifugation indicated that activity is restricted to light membranes Salvia officinalis
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no effect on enzyme activity by 1-10 mM of Mg2+, Mn2+, Ca2+, Cu2+, and Fe2+ Salvia officinalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-sabinene + NADPH + H+ + O2 Salvia officinalis
-
(+)-cis-sabinol + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Salvia officinalis
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
epidermis
-
Salvia officinalis
-
leaf
-
Salvia officinalis
-
seedling etiolated Salvia officinalis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-sabinene + NADPH + H+ + O2
-
Salvia officinalis (+)-cis-sabinol + NADP+ + H2O
-
?
(+)-sabinene + NADPH + H+ + O2 the hydroxylase is highly specific for (+)-sabinene as substrate. Addition of sabinene to solubilized microsomes provides a typical type I binding spectrum Salvia officinalis (+)-cis-sabinol + NADP+ + H2O GC product analysis ?

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
20
-
assay at Salvia officinalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
Salvia officinalis

pH Range

pH Minimum pH Maximum Comment Organism
6.8 8.6 50% of maximal activity at pH 6.8 and pH 8.6 Salvia officinalis

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Salvia officinalis

General Information

General Information Comment Organism
evolution the enzyme is a cytochrome P-450-dependent mixed function oxygenase and belongs to the cytochrome P450 oxygenase family Salvia officinalis
metabolism the enzyme catalyzes the synthesis of (+)-cis-sabinol, which is a key step in the biosynthesis of C3-oxygenated thujane monoterpenes, overview Salvia officinalis
additional information (+)-sabinene is a major olefinic constituent of the volatile oil of immature Salvia oficinalis Salvia officinalis