Activating Compound | Comment | Organism | Structure |
---|---|---|---|
FAD | exogenous, activates the enzyme slightly | Salvia officinalis | |
FMN | exogenous, activates the enzyme | Salvia officinalis | |
octyl beta-D-glucoside | 35% activation at 0.12% detergent, complete inhibition at 0.48% detergent | Salvia officinalis | |
sodium cholate | 40% activation at 0.30% detergent, 17% inhibition at 0.48% detergent | Salvia officinalis | |
Zwittergent 3-08 | 12% activation at 0.24% detergent | Salvia officinalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CO | photoreversible inhibition | Salvia officinalis | |
NADP+ | NADP+ competitively inhibits P-450-dependent reactions, 40-45% inhibition of hydroxylation at 2 mM | Salvia officinalis | |
octyl beta-D-glucoside | 35% activation at 0.12% detergent, complete inhibition at 0.48% detergent | Salvia officinalis | |
sodium cholate | 40% activation at 0.30% detergent, 17% inhibition at 0.48% detergent | Salvia officinalis | |
sodium deoxycholate | 80% inhibition at 0.06% detergent | Salvia officinalis | |
sodium taurocholate | 13% inhibition at 0.30% detergent | Salvia officinalis | |
sodium taurodeoxycholate | 91% inhibition at 0.06% detergent | Salvia officinalis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | localization of sabinene hydroxylase in sage leaf homogenates by differential centrifugation indicated that activity is restricted to light membranes | Salvia officinalis | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect on enzyme activity by 1-10 mM of Mg2+, Mn2+, Ca2+, Cu2+, and Fe2+ | Salvia officinalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-sabinene + NADPH + H+ + O2 | Salvia officinalis | - |
(+)-cis-sabinol + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salvia officinalis | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epidermis | - |
Salvia officinalis | - |
leaf | - |
Salvia officinalis | - |
seedling | etiolated | Salvia officinalis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-sabinene + NADPH + H+ + O2 | - |
Salvia officinalis | (+)-cis-sabinol + NADP+ + H2O | - |
? | |
(+)-sabinene + NADPH + H+ + O2 | the hydroxylase is highly specific for (+)-sabinene as substrate. Addition of sabinene to solubilized microsomes provides a typical type I binding spectrum | Salvia officinalis | (+)-cis-sabinol + NADP+ + H2O | GC product analysis | ? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Salvia officinalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Salvia officinalis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 8.6 | 50% of maximal activity at pH 6.8 and pH 8.6 | Salvia officinalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Salvia officinalis |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a cytochrome P-450-dependent mixed function oxygenase and belongs to the cytochrome P450 oxygenase family | Salvia officinalis |
metabolism | the enzyme catalyzes the synthesis of (+)-cis-sabinol, which is a key step in the biosynthesis of C3-oxygenated thujane monoterpenes, overview | Salvia officinalis |
additional information | (+)-sabinene is a major olefinic constituent of the volatile oil of immature Salvia oficinalis | Salvia officinalis |