BRENDA - Enzyme Database
show all sequences of 1.14.13.147

Taxol biosynthesis: Molecular cloning and characterization of a cytochrome P450 taxoid 7beta-hydroxylase

Chau, M.; Jennewein, S.; Walker, K.; Croteau, R.; Chem. Biol. 11, 663-672 (2004)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in a Spodoptera fugiperda-baculovirus-based expression system
Taxus cuspidata
General Stability
General Stability
Organism
taxhydroxylase activity is slightly higher in HEPES than in Tris-HCl, however, hydrolytic activity (enzymatic deacylation of both substrate and product) is also more prominent in HEPES7
Taxus cuspidata
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0076
-
(+)-taxusin
pH 7.5, temperature not specified in the publication
Taxus cuspidata
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Taxus cuspidata
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Taxus cuspidata
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
P-93AF cell
-
Taxus cuspidata
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-taxusin + O2 + NADPH + H+
i.e. taxadien-5alpha,9alpha,10beta,13alpha-tetraol tetraacetate
717479
Taxus cuspidata
7beta-hydroxytaxusin + NADP+ + H2O
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Taxus cuspidata
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Taxus cuspidata
NADPH
-
Taxus cuspidata
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in a Spodoptera fugiperda-baculovirus-based expression system
Taxus cuspidata
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Taxus cuspidata
NADPH
-
Taxus cuspidata
General Stability (protein specific)
General Stability
Organism
taxhydroxylase activity is slightly higher in HEPES than in Tris-HCl, however, hydrolytic activity (enzymatic deacylation of both substrate and product) is also more prominent in HEPES7
Taxus cuspidata
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0076
-
(+)-taxusin
pH 7.5, temperature not specified in the publication
Taxus cuspidata
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Taxus cuspidata
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
P-93AF cell
-
Taxus cuspidata
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-taxusin + O2 + NADPH + H+
i.e. taxadien-5alpha,9alpha,10beta,13alpha-tetraol tetraacetate
717479
Taxus cuspidata
7beta-hydroxytaxusin + NADP+ + H2O
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Taxus cuspidata
Other publictions for EC 1.14.13.147
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
718195
Kaspera
Cytochrome P450 oxygenases of ...
Taxus cuspidata
Phytochem. Rev.
5
433-444
2006
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717479
Chau
Taxol biosynthesis: Molecular ...
Taxus cuspidata
Chem. Biol.
11
663-672
2004
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