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Literature summary for 1.14.13.130 extracted from

  • Hormann, K; Andreesen, J.R.
    Purification and characterization of a pyrrole-2-carboxylate oxygenase from Arthrobacter strain Py (1994), Biol. Chem. Hoppe-Seyler, 375, 211-218.
    View publication on PubMed

General Stability

General Stability Organism
FAD and dithioerythritol stabilize enzyme activity during purification Arthrobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic acid) 0.5 mM, 87% loss of activity Arthrobacter sp.
Cu+ 0.001-0.01 mM, 93% loss of activity Arthrobacter sp.
Hg2+ 0.01-0.1 mM, 96% loss of activity Arthrobacter sp.
additional information no inhibition by 1 mM EDTA or 1 mM arsenite Arthrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
x * 60000, SDS-PAGE Arthrobacter sp.
160000
-
gel filtration Arthrobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyrrole-2-carboxylate + NADH + H+ + O2 Arthrobacter sp. the enzyme initiates the degradation of pyrrole-2-carboxylate. Growth on pyrrole-2-carboxylate as the sole source of carbon, nitrogen and energy 5-hydroxypyrrole-2-carboxylate + NAD+ + H2O
-
?
pyrrole-2-carboxylate + NADH + H+ + O2 Arthrobacter sp. Py1 the enzyme initiates the degradation of pyrrole-2-carboxylate. Growth on pyrrole-2-carboxylate as the sole source of carbon, nitrogen and energy 5-hydroxypyrrole-2-carboxylate + NAD+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
-
-
Arthrobacter sp. Py1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
FAD and dithioerythritol stabilize enzyme activity during purification Arthrobacter sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:pyrrole-2-carboxylate-grown cell
-
Arthrobacter sp.
-

Storage Stability

Storage Stability Organism
-20°C, several months, stable Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyrrole-2-carboxylate + NADH + H+ + O2 the enzyme initiates the degradation of pyrrole-2-carboxylate. Growth on pyrrole-2-carboxylate as the sole source of carbon, nitrogen and energy Arthrobacter sp. 5-hydroxypyrrole-2-carboxylate + NAD+ + H2O
-
?
pyrrole-2-carboxylate + NADH + H+ + O2 no activity with NADPH. Narrow substrate specificity. Besides pyrrole-2-carboxylate, only pyrrole, pyrrole-2-aldehyde, and indole-2-carboxylate stimulate the oxygen consumption at a very low rate Arthrobacter sp. 5-hydroxypyrrole-2-carboxylate + NAD+ + H2O
-
?
pyrrole-2-carboxylate + NADH + H+ + O2 the enzyme initiates the degradation of pyrrole-2-carboxylate. Growth on pyrrole-2-carboxylate as the sole source of carbon, nitrogen and energy Arthrobacter sp. Py1 5-hydroxypyrrole-2-carboxylate + NAD+ + H2O
-
?
pyrrole-2-carboxylate + NADH + H+ + O2 no activity with NADPH. Narrow substrate specificity. Besides pyrrole-2-carboxylate, only pyrrole, pyrrole-2-aldehyde, and indole-2-carboxylate stimulate the oxygen consumption at a very low rate Arthrobacter sp. Py1 5-hydroxypyrrole-2-carboxylate + NAD+ + H2O
-
?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 9
-
Arthrobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
7.2 9.5 pH 7.2: 65% of maximal activity, pH 9.5: 68% of maximal activity Arthrobacter sp.

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein. Contains 2.7-3.6 mol of FAD per mol of enzyme Arthrobacter sp.
NADH no activity with NADPH Arthrobacter sp.

pI Value

Organism Comment pI Value Maximum pI Value
Arthrobacter sp.
-
-
4.6

Expression

Organism Comment Expression
Arthrobacter sp. activity is induced by wrowth on pyrrole-2-carboxylate as the sole source of carbon, nitrogen and energy up

General Information

General Information Comment Organism
physiological function the enzyme initiates the degradation of pyrrole-2-carboxylate. Growth on pyrrole-2-carboxylate as the sole source of carbon, nitrogen and energy Arthrobacter sp.