General Stability | Organism |
---|---|
stabilization can be achieved by addition of either glycerol (20%), bovine serum albumin (10 mg/ml), dithiothreitol (1 mM) or the substrate 7-methylxanthine (1 mM). Storage under nitrogen atmosphere has a stabilizing influence. The addition of caffeine (1 mM), dimethylxanthines (1 mM) and protease inhibitors (pepstatin A, PMSF, 0.1 mM) is without influence on stability | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 0.2 mM, complete inhibition | Pseudomonas putida | |
Co2+ | 0.2 mM, 40% inhibition | Pseudomonas putida | |
Cu2+ | 0.2 mM, complete inhibition | Pseudomonas putida | |
Fe2+ | 1 mM, 37% inhibition | Pseudomonas putida | |
Hg2+ | 1 mM, 98% inhibition | Pseudomonas putida | |
Sn2+ | 0.2 mM, 17% inhibition | Pseudomonas putida | |
Zn2+ | 0.2 mM, complete inhibition | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.113 | - |
O2 | pH and temperature not specified in the publication | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, activation to 123% of control | Pseudomonas putida | |
Mg2+ | 1 mM, activation to 132% of control | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Pseudomonas putida |
Storage Stability | Organism |
---|---|
4°C, pH 6.0, 94% loss of activity after 24 h | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-methylxanthine + O2 + NADH + H+ | - |
Pseudomonas putida | xanthine + NAD+ + H2O + formaldehyde | - |
? | |
7-methylxanthine + O2 + NADPH + H+ | - |
Pseudomonas putida | xanthine + NADP+ + H2O + formaldehyde | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 35 | - |
Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
1 min, complete inactivation | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
- |
Pseudomonas putida |