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Literature summary for 1.14.13.122 extracted from

  • Reinbothe, C.; Bartsch, S.; Eggink, L.L.; Hoober, J.K.; Brusslan, J.; Andrade-Paz, R.; Monnet, J.; Reinbothe, S.
    A role for chlorophyllide a oxygenase in the regulated import and stabilization of light-harvesting chlorophyll a/b proteins (2006), Proc. Natl. Acad. Sci. USA, 103, 4777-4782.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.122

Cloned(Commentary)

Cloned (Comment) Organism
expression of His6-tagged CAO in Escherichia coli in inclusion bodies, in vitro expression by wheat germ lysates Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast intrinsic in inner envelope and thylakoid membrane Arabidopsis thaliana 9507
-
additional information the enzyme is part of a separate translocon complex in chlorplasts Arabidopsis thaliana
-
-
thylakoid membrane
-
 Arabidopsis thaliana 42651
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the enzyme contains a [2Fe-2S] Rieske cluster Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Arabidopsis thaliana the enzyme is part of a separate translocon complex involved in the regulated import and stabilization of the chlorophyllide b-binding light-harvesting proteins Lhcb1, of LHCII, and Lhcb4, of CP29, in chlorplasts ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
 gene CAO
-

Purification (Commentary)

Purification (Comment) Organism
refolded recombinant His6-tagged CAO from Escherichia coli by nickel affinity chromatography to about 85% purity Arabidopsis thaliana

Renatured (Commentary)

Renatured (Comment) Organism
solubilization of recombinant His6-tagged enzyme from inclusion bodies after expression in Escherichia coli by 8 M urea and refolding Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 the assay is performed in darkness Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-hydroxychlorophyllide a + O2 + NADPH
-
 Arabidopsis thaliana chlorophyllide b + H2O + NADP+
-
 ?
chlorophyllide a + O2 + NADPH + H+
-
 Arabidopsis thaliana 7-hydroxychlorophyllide a + H2O + NADP+
-
 ?
additional information the enzyme is part of a separate translocon complex involved in the regulated import and stabilization of the chlorophyllide b-binding light-harvesting proteins Lhcb1, of LHCII, and Lhcb4, of CP29, in chlorplasts Arabidopsis thaliana ?
-
 ?
protochlorophyllide a + O2 + NADPH low activity Arabidopsis thaliana 7-hydroxychlorophyllide a + H2O + NADP+
-
 ?

Subunits

Subunits Comment Organism
More the enzyme is part of a separate translocon complex in chlorplasts Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
CAO
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
 assay at Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NADPH using a ferredoxin-NADPH reduction system in activity assays Arabidopsis thaliana