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Literature summary for 1.14.13.101 extracted from

  • Sehlmeyer, S.; Wang, L.; Langel, D.; Heckel, D.G.; Mohagheghi, H.; Petschenka, G.; Ober, D.
    Flavin-dependent monooxygenases as a detoxification mechanism in insects: new insights from the arctiids (lepidoptera) (2010), PLoS ONE, 5, e10435.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressing GgPNO without the signal peptide in at least a partially soluble and active form. Betaine is added to the medium and promotes its uptake by osmotic stress by sorbitol. Further improvements are achieved by coexpression with the Escherichia coli chaperones DnaK/DnaJ/GrpE by using the plasmid pRDKJG and by reducing the expression temperature to 4°C Apantesis nevadensis geneura
the heterologous expression results in the formation of an insoluble and inactive protein. Despite the finding that protein solubilization and subsequent renaturation results in an active protein, the specific activity remains low. Active SNO is detected by using an an Sf9 insect cell expression system, but the yield of protein is too low for further biochemical characterization. Using an alignment of senecionine N-oxygenase of Tyria jacobaeae and of flavin-dependent monooxygenase-like sequences of the dipteran species Drosophila melanogaster and Anopheles gambiae, several new flavin-dependent monooxygenase sequences of arctiids and other Lepidoptera are idenified Tyria jacobaeae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
senecionine + NADPH + O2 Tyria jacobaeae
-
senecionine N-oxide + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Apantesis nevadensis geneura D6CHF2
-
-
Tyria jacobaeae Q8MP06
-
-

Source Tissue

Source Tissue Comment Organism Textmining
larva
-
Apantesis nevadensis geneura
-
larva fat body and head Tyria jacobaeae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.3
-
purified enzyme with senecionine as substrate Apantesis nevadensis geneura

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
atropine + NADPH + O2 21% of the activity with monocrotaline Apantesis nevadensis geneura atropine N-oxide + NADP+ + H2O
-
?
axillarine + NADPH + O2 69% of the activity with monocrotaline Apantesis nevadensis geneura axillarine N-oxide + NADP+ + H2O
-
?
heliotrine + NADPH + O2 49% of the activity with monocrotaline Apantesis nevadensis geneura heliotrine N-oxide + NADP+ + H2O
-
?
monocrotaline + NADPH + O2
-
Apantesis nevadensis geneura monocrotaline N-oxide + NADP+ + H2O
-
?
additional information the enzyme shows activity with glutathione (41% of the activity with monocrotaline) Apantesis nevadensis geneura ?
-
?
phalaenopsine + NADPH + O2 19% of the activity with monocrotaline Apantesis nevadensis geneura phalaenopsine N-oxide + NADP+ + H2O
-
?
rinderine + NADPH + O2 43% of the activity with monocrotaline Apantesis nevadensis geneura rinderine N-oxide + NADP+ + H2O
-
?
senecionine + NADPH + O2
-
Tyria jacobaeae senecionine N-oxide + NADP+ + H2O
-
?
senecionine + NADPH + O2 61% of the activity with monocrotaline Apantesis nevadensis geneura senecionine N-oxide + NADP+ + H2O
-
?
seneciphylline + NADPH + O2 59% of the activity with monocrotaline Apantesis nevadensis geneura seneciphylline N-oxide + NADP+ + H2O
-
?

Synonyms

Synonyms Comment Organism
GgPNO
-
Apantesis nevadensis geneura
TjSNO
-
Tyria jacobaeae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Apantesis nevadensis geneura

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Apantesis nevadensis geneura

Cofactor

Cofactor Comment Organism Structure
FAD
-
Tyria jacobaeae