Cloned (Comment) | Organism |
---|---|
expressing GgPNO without the signal peptide in at least a partially soluble and active form. Betaine is added to the medium and promotes its uptake by osmotic stress by sorbitol. Further improvements are achieved by coexpression with the Escherichia coli chaperones DnaK/DnaJ/GrpE by using the plasmid pRDKJG and by reducing the expression temperature to 4°C | Apantesis nevadensis geneura |
the heterologous expression results in the formation of an insoluble and inactive protein. Despite the finding that protein solubilization and subsequent renaturation results in an active protein, the specific activity remains low. Active SNO is detected by using an an Sf9 insect cell expression system, but the yield of protein is too low for further biochemical characterization. Using an alignment of senecionine N-oxygenase of Tyria jacobaeae and of flavin-dependent monooxygenase-like sequences of the dipteran species Drosophila melanogaster and Anopheles gambiae, several new flavin-dependent monooxygenase sequences of arctiids and other Lepidoptera are idenified | Tyria jacobaeae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
senecionine + NADPH + O2 | Tyria jacobaeae | - |
senecionine N-oxide + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Apantesis nevadensis geneura | D6CHF2 | - |
- |
Tyria jacobaeae | Q8MP06 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
larva | - |
Apantesis nevadensis geneura | - |
larva | fat body and head | Tyria jacobaeae | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.3 | - |
purified enzyme with senecionine as substrate | Apantesis nevadensis geneura |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
atropine + NADPH + O2 | 21% of the activity with monocrotaline | Apantesis nevadensis geneura | atropine N-oxide + NADP+ + H2O | - |
? | |
axillarine + NADPH + O2 | 69% of the activity with monocrotaline | Apantesis nevadensis geneura | axillarine N-oxide + NADP+ + H2O | - |
? | |
heliotrine + NADPH + O2 | 49% of the activity with monocrotaline | Apantesis nevadensis geneura | heliotrine N-oxide + NADP+ + H2O | - |
? | |
monocrotaline + NADPH + O2 | - |
Apantesis nevadensis geneura | monocrotaline N-oxide + NADP+ + H2O | - |
? | |
additional information | the enzyme shows activity with glutathione (41% of the activity with monocrotaline) | Apantesis nevadensis geneura | ? | - |
? | |
phalaenopsine + NADPH + O2 | 19% of the activity with monocrotaline | Apantesis nevadensis geneura | phalaenopsine N-oxide + NADP+ + H2O | - |
? | |
rinderine + NADPH + O2 | 43% of the activity with monocrotaline | Apantesis nevadensis geneura | rinderine N-oxide + NADP+ + H2O | - |
? | |
senecionine + NADPH + O2 | - |
Tyria jacobaeae | senecionine N-oxide + NADP+ + H2O | - |
? | |
senecionine + NADPH + O2 | 61% of the activity with monocrotaline | Apantesis nevadensis geneura | senecionine N-oxide + NADP+ + H2O | - |
? | |
seneciphylline + NADPH + O2 | 59% of the activity with monocrotaline | Apantesis nevadensis geneura | seneciphylline N-oxide + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GgPNO | - |
Apantesis nevadensis geneura |
TjSNO | - |
Tyria jacobaeae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Apantesis nevadensis geneura |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Apantesis nevadensis geneura |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Tyria jacobaeae |