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Literature summary for 1.14.13.10 extracted from

  • Holmes, P.E.; Rittenberg, S.C.
    The bacterial oxidation of nicotine. VII. Partial purification and properties of 2,6-dihydroxypyridine oxidase (1972), J. Biol. Chem., 247, 7622-7627.
    View publication on PubMed
Search for more literature for 1.14.13.10

Activating Compound

Activating Compound Comment Organism Structure
CO 35% activation Paenarthrobacter nicotinovorans
EDTA 38% activation at 0.05 mM Paenarthrobacter nicotinovorans
KCN 18% activation at 0.5 mM Paenarthrobacter nicotinovorans

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 50% inhibition at 0.017 mM Paenarthrobacter nicotinovorans
Hg2+ 50% inhibition at 0.005 mM Paenarthrobacter nicotinovorans
NaN3 50% inhibition at 2 mM Paenarthrobacter nicotinovorans
p-chloromercuribenzoate 50% inhibition at 0.01 mM Paenarthrobacter nicotinovorans
Zn2+ 50% inhibition at 0.7 mM Paenarthrobacter nicotinovorans

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Paenarthrobacter nicotinovorans
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
89000
-
 sucrose density gradient centrifugation Paenarthrobacter nicotinovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,6-dihydroxypyridine + NADH + O2 Paenarthrobacter nicotinovorans
-
 2,3,6-trihydroxypyridine + NAD+ + H2O
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone complete loss of activity Paenarthrobacter nicotinovorans
Ethanol stabilizes during preparative manipulations Paenarthrobacter nicotinovorans
Glycerol stabilizes during preparative manipulations Paenarthrobacter nicotinovorans

Organism

Organism UniProt Comment Textmining
Paenarthrobacter nicotinovorans
-
 formerly Arthrobacter oxidans
-

Purification (Commentary)

Purification (Comment) Organism
partial Paenarthrobacter nicotinovorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.16
-
-
 Paenarthrobacter nicotinovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,6-dihydroxypyridine + NADH + electron acceptor methylene blue and 2,6-dichlorophenol-indophenol can act as alternative electron acceptors Paenarthrobacter nicotinovorans 2,3,6-trihydroxypyridine + NAD+ + reduced electron acceptor
-
 ?
2,6-dihydroxypyridine + NADH + O2
-
 Paenarthrobacter nicotinovorans 2,3,6-trihydroxypyridine + NAD+ + H2O
-
 ?
2,6-dihydroxypyridine + NADH + O2
-
 Paenarthrobacter nicotinovorans 2,3,6-trihydroxypyridine + NAD+ + H2O pyridine ring cleavage follows in a second reaction ?
additional information 2-hydroxypyridine, 3-hydroxypyridine, 4-hydroxypyridine, 2,5-dihydroxypyridine, 2,6-dipicolinic acid, p-hydroxybenzoic acid, nicotinic acid and nicotine are no substrates for this enzyme Paenarthrobacter nicotinovorans ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Paenarthrobacter nicotinovorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
15
-
 30% loss of activity in 10 min Paenarthrobacter nicotinovorans
30
-
 complete loss of activity in 10 min Paenarthrobacter nicotinovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Paenarthrobacter nicotinovorans

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
 most stable in 50 mM potassium phosphate buffer Paenarthrobacter nicotinovorans

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Paenarthrobacter nicotinovorans
NADH
-
 Paenarthrobacter nicotinovorans
NADPH less effective than NADH Paenarthrobacter nicotinovorans