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Literature summary for 1.14.13.10 extracted from

  • Baitsch, D.; Sandu, C.; Brandsch, R.; Igloi, G.L.
    Gene cluster on pAO1 of Arthrobacter nicotinovorans involved in degradation of the plant alkaloid nicotine: cloning, purification, and characterization of 2,6-dihydroxypyridine 3-hydroxylase (2001), J. Bacteriol., 183, 5262-5267.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.10

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli XL-1 blue Paenarthrobacter nicotinovorans

General Stability

General Stability Organism
dialysis against 1.5 M guanidinium hydrochloride removes FAD, no renaturation possible Paenarthrobacter nicotinovorans

Inhibitors

Inhibitors Comment Organism Structure
2,3-dihydroxypyridine 100% inhibition at 0.1 mM, irreversible inhibitor Paenarthrobacter nicotinovorans
2,6-dihydroxynicotinamide 55% inhibition at 0.1 mM, reversible inhibitor Paenarthrobacter nicotinovorans
2,6-dimethoxypyridine 100% inhibition at 0.1 mM, irreversible inhibitor Paenarthrobacter nicotinovorans
2-Hydroxypyridine 48% inhibition at 0.1 mM, reversible inhibitor Paenarthrobacter nicotinovorans
resorcine 51% inhibition at 0.1 mM, reversible inhibitor Paenarthrobacter nicotinovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0083
-
 2,6-Dihydroxypyridine pH 8, 20°C Paenarthrobacter nicotinovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 gel filtration Paenarthrobacter nicotinovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,6-dihydroxypyridine + NADH + O2 Paenarthrobacter nicotinovorans
-
 2,3,6-trihydroxypyridine + NAD+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Paenarthrobacter nicotinovorans
-
 formerly Arthrobacter oxidans
-

Purification (Commentary)

Purification (Comment) Organism
-
 Paenarthrobacter nicotinovorans

Renatured (Commentary)

Renatured (Comment) Organism
after removal of FAD through precipitation with 50% (NH4)2SO4 at pH 2.0, incubation with 0.1 mM FAD leeds to 90% activity recovery Paenarthrobacter nicotinovorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
90
-
-
 Paenarthrobacter nicotinovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,6-dihydroxypyridine + NADH + O2
-
 Paenarthrobacter nicotinovorans 2,3,6-trihydroxypyridine + NAD+ + H2O
-
 ?
2,6-dihydroxypyridine + NADH + O2
-
 Paenarthrobacter nicotinovorans 2,3,6-trihydroxypyridine + NAD+ + H2O product dimerizes spontaneously in the presence of oxygen to form a blue pigment ?

Subunits

Subunits Comment Organism
homodimer
-
 Paenarthrobacter nicotinovorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
-
 Paenarthrobacter nicotinovorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 stable Paenarthrobacter nicotinovorans
52
-
 inactivation Paenarthrobacter nicotinovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Paenarthrobacter nicotinovorans

Cofactor

Cofactor Comment Organism Structure
FAD 2 mol FAD per dimer Paenarthrobacter nicotinovorans
NADH no reaction with NADPH Paenarthrobacter nicotinovorans