Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-hydroxy-2-naphthoate | strong competitive inhibition of salicylate hydroxylation | Sphingomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
salicylate | 30°C, pH 7.0 | Sphingomonas sp. | |
0.028 | - |
anthranilate | 30°C, pH 7.0 | Sphingomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | three-component Fe-S protein complex: the oxygenase component, designated PhnII, exhibits an alpha3beta3 heterohexameric structure and contains one Rieske-type [2Fe-2S] cluster and one mononuclear iron per alpha subunit | Sphingomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
oxygenase component PhnII | Sphingomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-hydroxy-2-naphthoate + NADH + 2 H+ + O2 | 137% of the activity with salicylate | Sphingomonas sp. | ? | - |
? | |
2,4-dihydroxybenzoate + NADH + 2 H+ + O2 | 91% of the activity with salicylate | Sphingomonas sp. | 1,2,4-trihydroxybenzene + NAD+ + H2O + CO2 | - |
? | |
2,6-dihydroxybenzoate + NADH + 2 H+ + O2 | 70% of the activity with salicylate | Sphingomonas sp. | 1,2,3-trihydroxybenzene + NAD+ + H2O + CO2 | - |
? | |
2-hydroxy-1-naphthoate + NADH + 2 H+ + O2 | 160% of the activity with salicylate | Sphingomonas sp. | ? | - |
? | |
3-methylsalicylate + NADH + 2 H+ + O2 | 76% of the activity with salicylate | Sphingomonas sp. | 3-methylcatechol + NAD+ + H2O + CO2 | - |
? | |
4-methylsalicylate + NADH + 2 H+ + O2 | 108% of the activity with salicylate | Sphingomonas sp. | 4-methylcatechol + NAD+ + H2O + CO2 | - |
? | |
5-chlorosalicylate + NADH + 2 H+ + O2 | 46% of the activity with salicylate | Sphingomonas sp. | 4-chlorocatechol + NAD+ + H2O + CO2 | - |
? | |
5-methylsalicylate + NADH + 2 H+ + O2 | 68.5% of the activity with salicylate | Sphingomonas sp. | 4-methylcatechol + NAD+ + H2O + CO2 | - |
? | |
5-nitrosalicylate + NADH + 2 H+ + O2 | 32% of the activity with salicylate | Sphingomonas sp. | 4-nitrocatechol + NAD+ + H2O + CO2 | - |
? | |
anthranilate + NADH + 2 H+ + O2 | 67% of the activity with salicylate | Sphingomonas sp. | 2-aminophenol + NAD+ + H2O + CO2 | - |
? | |
gentisate + NADH + 2 H+ + O2 | 99% of the activity with salicylate | Sphingomonas sp. | ? | - |
? | |
additional information | 1-hydroxy-2-naphthoate, which is an intermediate in phenanthrene degradation, is not hydroxylated by PhnII, but it induces a high rate of uncoupled oxidation of NADH | Sphingomonas sp. | ? | - |
? | |
salicylate + NADH + 2 H+ + O2 | - |
Sphingomonas sp. | catechol + NAD+ + H2O + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | three-component Fe-S protein complex: the oxygenase component, designated PhnII, exhibits an alpha3beta3 heterohexameric structure and contains one Rieske-type [2Fe-2S] cluster and one mononuclear iron per alpha subunit. In the presence of purified reductase (PhnA4) and ferredoxin (PhnA3) components, PhnII catalyzes the hydroxylation of salicylate to catechol | Sphingomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
salicylate 1-hydroxylase | - |
Sphingomonas sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00068 | - |
1-hydroxy-2-naphthoate | 30°C, pH 7.0 | Sphingomonas sp. |