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Literature summary for 1.14.13.1 extracted from

  • Nikodem, P.; Hecht, V.; Schlomann, M.; Pieper, D.H.
    New bacterial pathway for 4- and 5-chlorosalicylate degradation via 4-chlorocatechol and maleylacetate in Pseudomonas sp. strain MT1 (2003), J. Bacteriol., 185, 6790-6800.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.1

Organism

Organism UniProt Comment Textmining
Pseudomonas reinekei Q0VH44 gene salA
-

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:5-chlorosalicylate-grown cell
-
 Pseudomonas reinekei
-
culture condition:salicylate-grown cell
-
 Pseudomonas reinekei
-
additional information very low activity in succinate-grown cells Pseudomonas reinekei
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
120
-
 purified enzyme from 5-chlorosalicylate-grown cells, pH not specified in the publication, 25°C, substrate 3-chlorosalicylate Pseudomonas reinekei
120
-
 purified enzyme from salicylate-grown cells, pH not specified in the publication, 25°C, substrate salicylate Pseudomonas reinekei
190
-
 purified enzyme from 5-chlorosalicylate-grown cells, pH not specified in the publication, 25°C, substrate 5-chlorosalicylate Pseudomonas reinekei
240
-
 purified enzyme from 5-chlorosalicylate-grown cells, pH not specified in the publication, 25°C, substrate 4-chlorosalicylate Pseudomonas reinekei
460
-
 purified enzyme from 5-chlorosalicylate-grown cells, pH not specified in the publication, 25°C, substrate salicylate Pseudomonas reinekei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-chlorosalicylate + NADH + H+ + O2
-
 Pseudomonas reinekei 3-chlorocatechol + NAD+ + H2O + CO2
-
 ?
4-chlorosalicylate + NADH + H+ + O2
-
 Pseudomonas reinekei 4-chlorocatechol + NAD+ + H2O + CO2
-
 ?
5-chlorosalicylate + NADH + H+ + O2
-
 Pseudomonas reinekei 5-chlorocatechol + NAD+ + H2O + CO2
-
 ?
salicylate + NADH + H+ + O2
-
 Pseudomonas reinekei catechol + NAD+ + H2O + CO2
-
 ?

Synonyms

Synonyms Comment Organism
salicylate 1-hydroxylase
-
 Pseudomonas reinekei
salicylate hydroxylase
-
 Pseudomonas reinekei
SalOH
-
 Pseudomonas reinekei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Pseudomonas reinekei

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Pseudomonas reinekei

General Information

General Information Comment Organism
metabolism strain MT1 is capable of degrading 4- and 5-chlorosalicylates via 4-chlorocatechol, 3-chloromuconate, and maleylacetate by the chlorocatechol pathway, overview Pseudomonas reinekei