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Literature summary for 1.14.12.7 extracted from

  • Pinto, A.; Tarasev, M.; Ballou, D.P.
    Substitutions of the bridging aspartate 178 result in profound changes in the reactivity of the Rieske center of phthalate dioxygenase (2006), Biochemistry, 45, 9032-9041.
    View publication on PubMed
Search for more literature for 1.14.12.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain C41(DE3) Burkholderia cepacia

Protein Variants

Protein Variants Comment Organism
D178A the rates of oxidation of the reduced Rieske centers are decreased by more than 10000fold, 16% of wild type activity Burkholderia cepacia
D178N the rates of oxidation of the reduced Rieske centers are decreased by more than 10000fold, 7% of wild type activity Burkholderia cepacia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0002
-
 Phthalate wild type enzyme with its mononuclear center containing Fe(II) Burkholderia cepacia
0.0009
-
 Phthalate mutant enzyme D178A with its mononuclear center lacking Fe(II) Burkholderia cepacia
0.0011
-
 Phthalate mutant enzyme D178N with its mononuclear center lacking Fe(II) Burkholderia cepacia
0.0016
-
 Phthalate wild type enzyme with its mononuclear center lacking Fe(II) Burkholderia cepacia
0.0058
-
 Phthalate mutant enzyme D178N with its mononuclear center containing Fe(II) Burkholderia cepacia
0.0093
-
 Phthalate mutant enzyme D178A with its mononuclear center containing Fe(II) Burkholderia cepacia

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the mononuclear center contains Fe(II), contains 2.85 Fe atoms per monomer Burkholderia cepacia

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia
-
-
-
Burkholderia cepacia DB01
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Burkholderia cepacia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phthalate + NADH + O2
-
 Burkholderia cepacia cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,2-dicarboxylate + NAD+ + H2O
-
 ?
phthalate + NADH + O2
-
 Burkholderia cepacia DB01 cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,2-dicarboxylate + NAD+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
PDO
-
 Burkholderia cepacia

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Burkholderia cepacia