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Literature summary for 1.14.12.3 extracted from

  • Di Gennaro, P.; Bruzzese, N.; Anderlini, D.; Aiossa, M.; Papacchini, M.; Campanella, L.; Bestetti, G.
    Development of microbial engineered whole-cell systems for environmental benzene determination (2010), Ecotoxicol. Environ. Saf., 74, 542-549.
    View publication on PubMed

Application

Application Comment Organism
analysis the enzyme is used in a whole-cell assay as biosensor for benzene concentration and vapopur, overview Pseudomonas putida

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain DH5alpha and JM109, co-expression with benzene dihydrodiol dehydrogenase under the control of the Ptac promoter or without any induction. The recombinant strains expressing the BED and the BDDH enzymes transform benzene into dihydrodiol with corresponding consumption of oxygen and regenerate NADH by converting dihydrodiol to catechol Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
additional information establishing of a detection system to monitor environmental benzene contamination by co-expression of benzene dioxygenase with benzene dihydrodiol dehydrogenase in Escherichia coli. The procedures involving whole-cell bioassays determine the concentration of benzene through benzene dioxygenase activity, which allows for direct correlation of oxygen consumption, and through the benzene dihydrodiol dehydrogenase that causes catechol accumulation and restores NADH necessary for the activity of the first enzyme Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzene + NADH + H+ + O2 Pseudomonas putida
-
cis-cyclohexa-3,5-diene-1,2-diol + NAD+
-
?
benzene + NADH + H+ + O2 Pseudomonas putida MST
-
cis-cyclohexa-3,5-diene-1,2-diol + NAD+
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida MST
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzene + NADH + H+ + O2
-
Pseudomonas putida cis-cyclohexa-3,5-diene-1,2-diol + NAD+
-
?
benzene + NADH + H+ + O2
-
Pseudomonas putida MST cis-cyclohexa-3,5-diene-1,2-diol + NAD+
-
?

Synonyms

Synonyms Comment Organism
BED
-
Pseudomonas putida
benzene dioxygenase
-
Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NADH
-
Pseudomonas putida