Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is cloned under the strict control of the Palk promoter of Pseudomonas putida GPo1 and expressed in Escherichia coli JM101 cells carrying the resulting plasmid pTEZ30. Cells are used for regio- and stereoselective dihydroxylation of benzonitrile, ortho-, meta- and para-substituted benzonitriles, as well as cinnamonitrile and benzyl cyanide. cis-Dihydroxylations are at the 1,2 positions and the products have 42.9 to 97.1% enantiomeric excess | Pseudomonas sp. P51 |
Cloned (Comment) | Organism |
---|---|
cloned under the strict control of the Palk promoter of Pseudomonas putida GPo1. Escherichia coli JM101 cells carrying the resulting plasmid pTEZ30 are used for the biotransformation of benzonitrile in a 2-l stirred tank bioreactor | Pseudomonas sp. P51 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. P51 | Q52383 AND Q52384 AND Q52385 AND Q52386 | Q52383: large subunit (tcbAa), Q52384: small subunit (tcbAb), Q52385: ferredoxin (tcbAc), Q52386: NADH-ferredoxin reductase (tcbAd) | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-bromobenzonitrile + NADH + H+ + O2 | - |
Pseudomonas sp. P51 | cis-1,2-dihydroxy-3-cyanocyclohexa-4-bromo-3,5-diene + NAD+ | - |
? | |
2-chlorobenzonitrile + NADH + H+ + O2 | - |
Pseudomonas sp. P51 | cis-1,2-dihydroxy-3-cyanocyclohexa-4-chloro-3,5-diene + NAD+ | - |
? | |
2-fluorobenzonitrile + NADH + H+ + O2 | - |
Pseudomonas sp. P51 | cis-1,2-dihydroxy-3-cyanocyclohexa-4-fluoro-3,5-diene + NAD+ | - |
? | |
3-fluorobenzonitrile + NADH + H+ + O2 | - |
Pseudomonas sp. P51 | cis-1,2-dihydroxy-3-cyanocyclohexa-5-fluoro-3,5-diene + NAD+ | - |
? | |
4-chlorobenzonitrile + NADH + H+ + O2 | - |
Pseudomonas sp. P51 | cis-1,2-dihydroxy-3-cyanocyclohexa-6-chloro-3,5-diene + NAD+ | - |
? | |
4-fluorobenzonitrile + NADH + H+ + O2 | - |
Pseudomonas sp. P51 | cis-1,2-dihydroxy-3-cyanocyclohexa-6-fluoro-3,5-diene + NAD+ | - |
? | |
4-methylbenzonitrile + NADH + H+ + O2 | - |
Pseudomonas sp. P51 | cis-1,2-dihydroxy-3-cyanocyclohexa-6-methyl-3,5-diene + NAD+ | - |
? | |
benzonitrile + NADH + H+ + O2 | - |
Pseudomonas sp. P51 | cis-1,2-dihydroxy-3-cyanocyclohexa-3,5-diene + NAD+ | - |
? | |
benzyl cyanide + NADH + H+ + O2 | - |
Pseudomonas sp. P51 | cis-1,2-dihydroxy-3,5-cyclohexadienyl acetonitrile + NAD+ | - |
? | |
cinnamonitrile + NADH + H+ + O2 | - |
Pseudomonas sp. P51 | trans-3-[(5S,6R)-5,6-dihydroxycyclo-hexa-1,3-dienyl]-acrylonitrile + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CDO | - |
Pseudomonas sp. P51 |