Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of hybrid dioxygenases with the genes encoding 2-nitrotoluene 2,3-dioxygenase from Pseudomonas sp. strain JS42 and 2,4-dinitrotoluene dioxygenase from Burkholderia sp. strain DNT. The C-terminal region of the large subunit of the oxygenase component is responsible for the enzyme specificity differences observed between 2-nitrotoluene 2,3-dioxygenase and 2,4-dinitrotoluene dioxygenase | Burkholderia sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia sp. | - |
- |
- |
Burkholderia sp. DNT | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dinitrotoluene + NADH + O2 | - |
Burkholderia sp. | 4-methyl-5-nitrocatechol + nitrite + NAD+ | formation of 9.54 nmol 3-methylcatechol per mg protein plus 1.06 nmol 2-nitrobenzyl alcohol | ? | |
2,4-dinitrotoluene + NADH + O2 | - |
Burkholderia sp. DNT | 4-methyl-5-nitrocatechol + nitrite + NAD+ | formation of 9.54 nmol 3-methylcatechol per mg protein plus 1.06 nmol 2-nitrobenzyl alcohol | ? | |
3-nitrotoluene + NADH + O2 | - |
Burkholderia sp. | 4-methylcatechol + nitrite + NAD+ | formation of 0.06 nmol 4-methylcatechol per mg protein plus 0.03 nmol 3-nitrobenzyl alcohol | ? | |
3-nitrotoluene + NADH + O2 | - |
Burkholderia sp. DNT | 4-methylcatechol + nitrite + NAD+ | formation of 0.06 nmol 4-methylcatechol per mg protein plus 0.03 nmol 3-nitrobenzyl alcohol | ? | |
additional information | no substrate: 2-nitrotoluene, 3-nitrotoluene | Burkholderia sp. | ? | - |
? | |
additional information | no substrate: 2-nitrotoluene, 3-nitrotoluene | Burkholderia sp. DNT | ? | - |
? | |
naphthalene + O2 | - |
Burkholderia sp. | cis-1,2-dihydroxy-1,2-dihydronaphthalene | formation of 0.83 nmol 3-methylcatechol per mg protein | ? | |
naphthalene + O2 | - |
Burkholderia sp. DNT | cis-1,2-dihydroxy-1,2-dihydronaphthalene | formation of 0.83 nmol 3-methylcatechol per mg protein | ? |
Synonyms | Comment | Organism |
---|---|---|
2,4-dinitrotoluene dioxygenase | - |
Burkholderia sp. |
2,4-DNTDO | - |
Burkholderia sp. |