Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Diaphorobacter sp. DS2 |
Protein Variants | Comment | Organism |
---|---|---|
additional information | individual expression of each subunit and separate purification. The active recombinant enzyme can be reconstituted in vitro by mixing all three purified subunits | Diaphorobacter sp. DS2 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Diaphorobacter sp. DS2 | M9PTH9 and M9PW10 and M9PSS0 and M9PV03 | M9PTH9 i.e. ferredoxin reductase subunit MntAa, M9PW10 i.e. oxygenase alpha subunit MntAc, M9PSS0 i.e. ferredoxin subunit MntAb, M9PV03 i.e. oxygenase beta subunit MntAd | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,6-dinitrotoluene + NADH + O2 | - |
Diaphorobacter sp. DS2 | 3-methyl-4-nitrocatechol + nitrite + NAD+ | 84% of the activity with 3-nitrotoluene | ? | |
2-chloronitrobenzene + NADH + O2 | - |
Diaphorobacter sp. DS2 | 3-chlorocatechol + nitrite + NAD+ | 80% of the activity with 3-nitrotoluene | ? | |
2-nitrotoluene + NADH + O2 | - |
Diaphorobacter sp. DS2 | 3-methylcatechol + nitrite + NAD+ | 71% of the activity with 3-nitrotoluene. Products are 91% 3-methylcatechol, 9% 3-nitrobenzyl alcohol | ? | |
3-nitrotoluene + NADH + O2 | - |
Diaphorobacter sp. DS2 | 3-methylcatechol + 4-methylcatechol + nitrite + NAD+ | products are 54% 3-methylcatechol, 39% 4-methylcatechol, 7% 3-nitrobenzyl alcohol | ? | |
nitrobenzene + NADH + O2 | - |
Diaphorobacter sp. DS2 | catechol + nitrite + NAD+ | 78% of the activity with 3-nitrotoluene | ? |
Synonyms | Comment | Organism |
---|---|---|
MntAa | - |
Diaphorobacter sp. DS2 |
MntAb | - |
Diaphorobacter sp. DS2 |
MntAc | - |
Diaphorobacter sp. DS2 |
MntAd | - |
Diaphorobacter sp. DS2 |