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Literature summary for 1.14.12.23 extracted from

  • Mahan, K.M.; Penrod, J.T.; Ju, K.S.; Al Kass, N.; Tan, W.A.; Truong, R.; Parales, J.V.; Parales, R.E.
    Selection for growth on 3-nitrotoluene by 2-nitrotoluene-utilizing Acidovorax sp. strain JS42 identifies nitroarene dioxygenases with altered specificities (2015), Appl. Environ. Microbiol., 81, 309-319.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.12.23

Protein Variants

Protein Variants Comment Organism
A405G synthesizes 17% 3-methylcatechol, 83% 4-methylcatechol from substrate 3-nitrotoluene Acidovorax sp.
I204A synthesizes 2% 3-methylcatechol, 97% 4-methylcatechol and 1% 3-nitrobenzyl alcohol from substrate 3-nitrotoluene Acidovorax sp.
I204T synthesizes 5% 3-methylcatechol, 93% 4-methylcatechol and 2% 3-nitrobenzyl alcohol from substrate 3-nitrotoluene Acidovorax sp.
I204V synthesizes 13% 3-methylcatechol, 83% 4-methylcatechol and 5% 3-nitrobenzyl alcohol from substrate 3-nitrotoluene Acidovorax sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
 2-nitrotoluene mutant A405G, pH 6.8, 30°C Acidovorax sp.
0.003
-
 2-nitrotoluene mutant I204V, pH 6.8, 30°C Acidovorax sp.
0.0038
-
 2-nitrotoluene wild-type, pH 6.8, 30°C Acidovorax sp.
0.0039
-
 3-nitrotoluene mutant I204V, pH 6.8, 30°C Acidovorax sp.
0.0042
-
 3-nitrotoluene pH 6.8, 30°C Acidovorax sp.
0.0045
-
 nitrobenzene pH 6.8, 30°C Acidovorax sp.
0.0047
-
 nitrobenzene wild-type, pH 6.8, 30°C Acidovorax sp.
0.0048
-
 nitrobenzene mutant I204V, pH 6.8, 30°C Acidovorax sp.
0.0054
-
 3-nitrotoluene mutant I204T, pH 6.8, 30°C Acidovorax sp.
0.0057
-
 2-nitrotoluene mutant I204T, pH 6.8, 30°C Acidovorax sp.
0.0062
-
 nitrobenzene mutant A405G, pH 6.8, 30°C Acidovorax sp.
0.0078
-
 3-nitrotoluene mutant A405G, pH 6.8, 30°C Acidovorax sp.
0.0092
-
 3-nitrotoluene mutant I204A, pH 6.8, 30°C Acidovorax sp.
0.0099
-
 nitrobenzene mutant I204T, pH 6.8, 30°C Acidovorax sp.
0.0137
-
 2-nitrotoluene pH 6.8, 30°C Acidovorax sp.
0.019
-
 2-nitrotoluene mutant I204A, pH 6.8, 30°C Acidovorax sp.
0.04
-
 3-nitrotoluene wild-type, pH 6.8, 30°C Acidovorax sp.
0.047
-
 nitrobenzene mutant I204A, pH 6.8, 30°C Acidovorax sp.

Organism

Organism UniProt Comment Textmining
Acidovorax sp.
-
-
-
Acidovorax sp. JS42
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrotoluene + NADH + O2
-
 Acidovorax sp. 3-methylcatechol + nitrite + NAD+
-
 ?
2-nitrotoluene + NADH + O2
-
 Acidovorax sp. JS42 3-methylcatechol + nitrite + NAD+
-
 ?
3-nitrotoluene + NADH + O2
-
 Acidovorax sp. 3-methylcatechol + nitrite + NAD+ products are 72% 3-methylcatechol, 25% 4-methylcatechol and 4% 3-nitrobenzyl alcohol ?
3-nitrotoluene + NADH + O2
-
 Acidovorax sp. JS42 3-methylcatechol + nitrite + NAD+ products are 72% 3-methylcatechol, 25% 4-methylcatechol and 4% 3-nitrobenzyl alcohol ?
3-nitrotoluene + NADH + O2
-
 Acidovorax sp. 4-methylcatechol + nitrite + NAD+ sole product ?
3-nitrotoluene + NADH + O2
-
 Acidovorax sp. JS42 4-methylcatechol + nitrite + NAD+ sole product ?
4-nitrotoluene + NADH + O2
-
 Acidovorax sp. 4-methylcatechol + nitrite + NAD+
-
 ?
4-nitrotoluene + NADH + O2
-
 Acidovorax sp. JS42 4-methylcatechol + nitrite + NAD+
-
 ?
additional information all three mononitrotoluene isomers serve as substrates for 2-nitrotoluene dioxygenase, but strain JS42 is unable to grow on 3- or 4-nitrotoluene Acidovorax sp. ?
-
 ?
additional information all three mononitrotoluene isomers serve as substrates for 2-nitrotoluene dioxygenase, but strain JS42 is unable to grow on 3- or 4-nitrotoluene Acidovorax sp. JS42 ?
-
 ?
nitrobenzene + NADH + O2
-
 Acidovorax sp. catechol + nitrite + NAD+
-
 ?
nitrobenzene + NADH + O2
-
 Acidovorax sp. catechol + nitrite + NAD+ reaction of EC 1.14.12.23 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.12
-
 2-nitrotoluene mutant I204A, pH 6.8, 30°C Acidovorax sp.
0.13
-
 3-nitrotoluene mutant A405G, pH 6.8, 30°C Acidovorax sp.
0.15
-
 3-nitrotoluene wild-type, pH 6.8, 30°C Acidovorax sp.
0.16
-
 2-nitrotoluene pH 6.8, 30°C Acidovorax sp.
0.19
-
 2-nitrotoluene mutant A405G, pH 6.8, 30°C Acidovorax sp.
0.19
-
 nitrobenzene mutant I204A, pH 6.8, 30°C Acidovorax sp.
0.21
-
 3-nitrotoluene mutant I204T, pH 6.8, 30°C Acidovorax sp.
0.21
-
 2-nitrotoluene mutant I204T, pH 6.8, 30°C Acidovorax sp.
0.22
-
 nitrobenzene mutant I204T, pH 6.8, 30°C Acidovorax sp.
0.23
-
 nitrobenzene mutant A405G, pH 6.8, 30°C Acidovorax sp.
0.25
-
 nitrobenzene mutant I204V, pH 6.8, 30°C Acidovorax sp.
0.25
-
 2-nitrotoluene mutant I204V, pH 6.8, 30°C Acidovorax sp.
0.26
-
 3-nitrotoluene mutant I204V, pH 6.8, 30°C Acidovorax sp.
0.27
-
 3-nitrotoluene pH 6.8, 30°C Acidovorax sp.
0.27
-
 3-nitrotoluene mutant I204A, pH 6.8, 30°C Acidovorax sp.
0.27
-
 nitrobenzene wild-type, pH 6.8, 30°C Acidovorax sp.
0.3
-
 nitrobenzene pH 6.8, 30°C Acidovorax sp.
0.34
-
 2-nitrotoluene wild-type, pH 6.8, 30°C Acidovorax sp.

General Information

General Information Comment Organism
physiological function Acidovorax sp. strain JS42 uses 2-nitrotoluene as a sole source of carbon and energy. The first enzyme of the degradation pathway, 2-nitrotoluene 2,3-dioxygenase, adds both atoms of molecular oxygen to 2-nitrotoluene, forming nitrite and 3-methylcatechol Acidovorax sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.7
-
 3-nitrotoluene wild-type, pH 6.8, 30°C Acidovorax sp.
4
-
 nitrobenzene mutant I204A, pH 6.8, 30°C Acidovorax sp.
6.5
-
 2-nitrotoluene mutant I204A, pH 6.8, 30°C Acidovorax sp.
12
-
 2-nitrotoluene pH 6.8, 30°C Acidovorax sp.
17
-
 3-nitrotoluene mutant A405G, pH 6.8, 30°C Acidovorax sp.
22
-
 nitrobenzene mutant I204T, pH 6.8, 30°C Acidovorax sp.
29
-
 3-nitrotoluene mutant I204A, pH 6.8, 30°C Acidovorax sp.
37
-
 nitrobenzene mutant A405G, pH 6.8, 30°C Acidovorax sp.
37
-
 2-nitrotoluene mutant I204T, pH 6.8, 30°C Acidovorax sp.
39
-
 3-nitrotoluene mutant I204T, pH 6.8, 30°C Acidovorax sp.
53
-
 nitrobenzene mutant I204V, pH 6.8, 30°C Acidovorax sp.
58
-
 nitrobenzene wild-type, pH 6.8, 30°C Acidovorax sp.
65
-
 3-nitrotoluene pH 6.8, 30°C Acidovorax sp.
67
-
 nitrobenzene pH 6.8, 30°C Acidovorax sp.
68
-
 3-nitrotoluene mutant I204V, pH 6.8, 30°C Acidovorax sp.
83
-
 2-nitrotoluene mutant I204V, pH 6.8, 30°C Acidovorax sp.
90
-
 2-nitrotoluene wild-type, pH 6.8, 30°C Acidovorax sp.
160
-
 2-nitrotoluene mutant A405G, pH 6.8, 30°C Acidovorax sp.