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Literature summary for 1.14.12.22 extracted from

  • Inoue, K.; Usami, Y.; Ashikawa, Y.; Noguchi, H.; Umeda, T.; Yamagami-Ashikawa, A.; Horisaki, T.; Uchimura, H.; Terada, T.; Nakamura, S.; Shimizu, K.; Habe, H.; Yamane, H.; Fujimoto, Z.; Nojiri, H.
    Structural basis of the divergent oxygenation reactions catalyzed by the rieske nonheme iron oxygenase carbazole 1,9a-dioxygenase (2014), Appl. Environ. Microbiol., 80, 2821-2832 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Janthinobacterium sp. J3

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.1M ammonium acetate, 12.5% (w/v) polyethylene glycol 3350 in 0.05 M MES at pH 5.5-5.7 Janthinobacterium sp. J3

Protein Variants

Protein Variants Comment Organism
F275W the mutant catalyzes the lateral dioxygenation of carbazole with higher yields than the wild type enzyme Janthinobacterium sp. J3
I262V the mutant catalyzes the lateral dioxygenation of carbazole with higher yields than the wild type enzyme Janthinobacterium sp. J3
Q282Y the mutant catalyzes the lateral dioxygenation of carbazole with higher yields than the wild type enzyme Janthinobacterium sp. J3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0507
-
9H-carbazole mutant enzyme I262V, at pH 7.5 and 30°C Janthinobacterium sp. J3
0.0546
-
9H-carbazole mutant enzyme Q282N, at pH 7.5 and 30°C Janthinobacterium sp. J3
0.0548
-
9H-carbazole mutant enzyme Q282Y, at pH 7.5 and 30°C Janthinobacterium sp. J3

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
9H-carbazole + NADH + H+ + O2 Janthinobacterium sp. J3
-
2'-aminobiphenyl-2,3-diol + NAD+
-
?
9H-carbazole + NADPH + H+ + O2 Janthinobacterium sp. J3
-
2'-aminobiphenyl-2,3-diol + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Janthinobacterium sp. J3 Q84II6
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap chelation column chromatography and Superdex 200 gel filtration Janthinobacterium sp. J3

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
9H-carbazole + NADH + H+ + O2
-
Janthinobacterium sp. J3 2'-aminobiphenyl-2,3-diol + NAD+
-
?
9H-carbazole + NADPH + H+ + O2
-
Janthinobacterium sp. J3 2'-aminobiphenyl-2,3-diol + NADP+
-
?
anthracene + NADH + H+ + O2
-
Janthinobacterium sp. J3 ?
-
?
biphenyl + NADH + H+ + O2
-
Janthinobacterium sp. J3 ?
-
?
dibenzothiophene + NADH + H+ + O2
-
Janthinobacterium sp. J3 ?
-
?
dibenzo[1,4]dioxin + NADH + H+ + O2
-
Janthinobacterium sp. J3 ?
-
?
fluorene + NADH + H+ + O2
-
Janthinobacterium sp. J3 ?
-
?
naphthalene + NADH + H+ + O2
-
Janthinobacterium sp. J3 ?
-
?

Synonyms

Synonyms Comment Organism
CARDO
-
Janthinobacterium sp. J3

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4
-
9H-carbazole mutant enzyme Q282N, at pH 7.5 and 30°C Janthinobacterium sp. J3
4.62
-
9H-carbazole mutant enzyme I262V, at pH 7.5 and 30°C Janthinobacterium sp. J3
5.49
-
9H-carbazole mutant enzyme Q282Y, at pH 7.5 and 30°C Janthinobacterium sp. J3

Cofactor

Cofactor Comment Organism Structure
NADH
-
Janthinobacterium sp. J3
NADPH
-
Janthinobacterium sp. J3
[2Fe-2S]-center
-
Janthinobacterium sp. J3

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.000733
-
9H-carbazole mutant enzyme Q282N, at pH 7.5 and 30°C Janthinobacterium sp. J3
0.00091
-
9H-carbazole mutant enzyme I262V, at pH 7.5 and 30°C Janthinobacterium sp. J3
0.001
-
9H-carbazole mutant enzyme Q282Y, at pH 7.5 and 30°C Janthinobacterium sp. J3