Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Janthinobacterium sp. J3 |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.1M ammonium acetate, 12.5% (w/v) polyethylene glycol 3350 in 0.05 M MES at pH 5.5-5.7 | Janthinobacterium sp. J3 |
Protein Variants | Comment | Organism |
---|---|---|
F275W | the mutant catalyzes the lateral dioxygenation of carbazole with higher yields than the wild type enzyme | Janthinobacterium sp. J3 |
I262V | the mutant catalyzes the lateral dioxygenation of carbazole with higher yields than the wild type enzyme | Janthinobacterium sp. J3 |
Q282Y | the mutant catalyzes the lateral dioxygenation of carbazole with higher yields than the wild type enzyme | Janthinobacterium sp. J3 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0507 | - |
9H-carbazole | mutant enzyme I262V, at pH 7.5 and 30°C | Janthinobacterium sp. J3 | |
0.0546 | - |
9H-carbazole | mutant enzyme Q282N, at pH 7.5 and 30°C | Janthinobacterium sp. J3 | |
0.0548 | - |
9H-carbazole | mutant enzyme Q282Y, at pH 7.5 and 30°C | Janthinobacterium sp. J3 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
9H-carbazole + NADH + H+ + O2 | Janthinobacterium sp. J3 | - |
2'-aminobiphenyl-2,3-diol + NAD+ | - |
? | |
9H-carbazole + NADPH + H+ + O2 | Janthinobacterium sp. J3 | - |
2'-aminobiphenyl-2,3-diol + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Janthinobacterium sp. J3 | Q84II6 | - |
- |
Purification (Comment) | Organism |
---|---|
HiTrap chelation column chromatography and Superdex 200 gel filtration | Janthinobacterium sp. J3 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
9H-carbazole + NADH + H+ + O2 | - |
Janthinobacterium sp. J3 | 2'-aminobiphenyl-2,3-diol + NAD+ | - |
? | |
9H-carbazole + NADPH + H+ + O2 | - |
Janthinobacterium sp. J3 | 2'-aminobiphenyl-2,3-diol + NADP+ | - |
? | |
anthracene + NADH + H+ + O2 | - |
Janthinobacterium sp. J3 | ? | - |
? | |
biphenyl + NADH + H+ + O2 | - |
Janthinobacterium sp. J3 | ? | - |
? | |
dibenzothiophene + NADH + H+ + O2 | - |
Janthinobacterium sp. J3 | ? | - |
? | |
dibenzo[1,4]dioxin + NADH + H+ + O2 | - |
Janthinobacterium sp. J3 | ? | - |
? | |
fluorene + NADH + H+ + O2 | - |
Janthinobacterium sp. J3 | ? | - |
? | |
naphthalene + NADH + H+ + O2 | - |
Janthinobacterium sp. J3 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CARDO | - |
Janthinobacterium sp. J3 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4 | - |
9H-carbazole | mutant enzyme Q282N, at pH 7.5 and 30°C | Janthinobacterium sp. J3 | |
4.62 | - |
9H-carbazole | mutant enzyme I262V, at pH 7.5 and 30°C | Janthinobacterium sp. J3 | |
5.49 | - |
9H-carbazole | mutant enzyme Q282Y, at pH 7.5 and 30°C | Janthinobacterium sp. J3 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Janthinobacterium sp. J3 | |
NADPH | - |
Janthinobacterium sp. J3 | |
[2Fe-2S]-center | - |
Janthinobacterium sp. J3 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000733 | - |
9H-carbazole | mutant enzyme Q282N, at pH 7.5 and 30°C | Janthinobacterium sp. J3 | |
0.00091 | - |
9H-carbazole | mutant enzyme I262V, at pH 7.5 and 30°C | Janthinobacterium sp. J3 | |
0.001 | - |
9H-carbazole | mutant enzyme Q282Y, at pH 7.5 and 30°C | Janthinobacterium sp. J3 |