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Literature summary for 1.14.12.18 extracted from

  • Zielinski, M.; Kahl, S.; Hecht, H.J.; Hofer, B.
    Pinpointing biphenyl dioxygenase residues that are crucial for substrate interaction (2003), J. Bacteriol., 185, 6976-6980.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
A234S significant change in regiospecificity of substrate dioxygenation, factor 2.3 Burkholderia sp.
F332A significant change in regiospecificity of substrate dioxygenation Burkholderia sp.
F378A very strong change in regiospecificity of substrate dioxygenation, factor higher than 7 Burkholderia sp.
F384A very strong change in regiospecificity of substrate dioxygenation, factor higher than 7 Burkholderia sp.
G389A significant change in regiospecificity of substrate dioxygenation Burkholderia sp.
I243A significant change in regiospecificity of substrate dioxygenation Burkholderia sp.
I326A significant change in regiospecificity of substrate dioxygenation Burkholderia sp.
M231A very strong change in regiospecificity of substrate dioxygenation, factor higher than 7 Burkholderia sp.
M324A significant change in regiospecificity of substrate dioxygenation Burkholderia sp.
N377A significant change in regiospecificity of substrate dioxygenation Burkholderia sp.
P334A significant change in regiospecificity of substrate dioxygenation Burkholderia sp.
V383S significant change in regiospecificity of substrate dioxygenation Burkholderia sp.
V393S significant change in regiospecificity of substrate dioxygenation Burkholderia sp.
W342A significant change in regiospecificity of substrate dioxygenation Burkholderia sp.
W392A significant change in regiospecificity of substrate dioxygenation Burkholderia sp.

Organism

Organism UniProt Comment Textmining
Burkholderia sp.
-
-
-
Burkholderia sp. LB400
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3'-dichlorobiphenyl + NADH + H+ + O2
-
Burkholderia sp. 3-chloro-5-(2-chlorophenyl)cyclohexa-3,5-diene-1,2-diol + 4-chloro-3-(3-chlorophenyl)cyclohexa-3,5-diene-1,2-diol + 5-chloro-3-(2-chlorophenyl)cyclohexa-3,5-diene-1,2-diol
-
?
2,3'-dichlorobiphenyl + NADH + H+ + O2
-
Burkholderia sp. LB400 3-chloro-5-(2-chlorophenyl)cyclohexa-3,5-diene-1,2-diol + 4-chloro-3-(3-chlorophenyl)cyclohexa-3,5-diene-1,2-diol + 5-chloro-3-(2-chlorophenyl)cyclohexa-3,5-diene-1,2-diol
-
?
3,4'-dichlorobiphenyl + NADH + H+ + O2
-
Burkholderia sp. 3-chloro-5-(4-chlorophenyl)cyclohexa-3,5-diene-1,2-diol + 3-chloro-6-(3-chlorophenyl)cyclohexa-3,4-diene-1,2-diol
-
?
3,4'-dichlorobiphenyl + NADH + H+ + O2
-
Burkholderia sp. LB400 3-chloro-5-(4-chlorophenyl)cyclohexa-3,5-diene-1,2-diol + 3-chloro-6-(3-chlorophenyl)cyclohexa-3,4-diene-1,2-diol
-
?