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Literature summary for 1.14.12.18 extracted from

  • Chebrou, H.; Hurtubise, Y.; Barriault, D.; Sylvestre, M.
    Heterologous expression and characterization of the purified oxygenase component of Rhodococcus globerulus P6 biphenyl dioxygenase and of chimeras derived from it (1999), J. Bacteriol., 181, 4805-4811.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.12.18

Cloned(Commentary)

Cloned (Comment) Organism
in Escherichia coli strain M15 and SG13009 Rhodococcus globerulus
in Pseudomonas putida strain KT2442 Rhodococcus globerulus

Organism

Organism UniProt Comment Textmining
Rhodococcus globerulus
-
-
-
Rhodococcus globerulus P6
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-tagged ISP, expressed in Escherichia coli and Pseudomonas putida Rhodococcus globerulus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-dichlorobiphenyl + NADH + O2
-
 Rhodococcus globerulus 5,6-dihydroxy-1-phenylcyclohexa-1,3-diene + 2,3-dihydroxy-2'-chlorobiphenyl + NAD+ + HCl
-
 ?
2,2'-dichlorobiphenyl + NADH + O2
-
 Rhodococcus globerulus P6 5,6-dihydroxy-1-phenylcyclohexa-1,3-diene + 2,3-dihydroxy-2'-chlorobiphenyl + NAD+ + HCl
-
 ?
2,5-dichlorobiphenyl + NADH + O2
-
 Rhodococcus globerulus cis-2',3'-dihydroxy-1'-(2,5-dichlorophenyl)-cyclohexa-4',6'-diene + 3,4-dihydroxy-1-phenylcyclohexa-1,5-diene + NAD+ + HCl
-
 ?
2,5-dichlorobiphenyl + NADH + O2
-
 Rhodococcus globerulus P6 cis-2',3'-dihydroxy-1'-(2,5-dichlorophenyl)-cyclohexa-4',6'-diene + 3,4-dihydroxy-1-phenylcyclohexa-1,5-diene + NAD+ + HCl
-
 ?
3,3'-dichlorobiphenyl + NADH + O2
-
 Rhodococcus globerulus 5,6-dihydroxy-1-phenylcyclohexa-1,3-diene + 4,5-dihydroxy-1-phenylcyclohexa-1,2-diene + NAD+ + HCl
-
 ?
3,3'-dichlorobiphenyl + NADH + O2
-
 Rhodococcus globerulus P6 5,6-dihydroxy-1-phenylcyclohexa-1,3-diene + 4,5-dihydroxy-1-phenylcyclohexa-1,2-diene + NAD+ + HCl
-
 ?
biphenyl + NAD(P)H + O2
-
 Rhodococcus globerulus 2,3-dihydro-dihydroxybiphenyl + NAD(P)+
-
 ?
biphenyl + NAD(P)H + O2
-
 Rhodococcus globerulus P6 2,3-dihydro-dihydroxybiphenyl + NAD(P)+
-
 ?