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Literature summary for 1.14.12.18 extracted from
- Purification and characterization of the NADH:ferredoxinBPH oxidoreductase component of biphenyl 2,3-dioxygenase from Pseudomonas sp. strain LB400 (1998), Arch. Microbiol., 170, 106-112.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.058 | - |
NADH | recombinant, purified NADH: ferredoxin oxidoreductase component | Pseudomonas sp. |  |
| 0.156 | - |
NADPH | recombinant, purified NADH: ferredoxin oxidoreductase component | Pseudomonas sp. | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | - |
Pseudomonas sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41500 | - |
NADH: ferredoxin component, gel filtration with Superose 6 | Pseudomonas sp. |
| 43600 | - |
NADH: ferredoxin component, SDS-PAGE | Pseudomonas sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas sp. | - |
- |
- |
| Pseudomonas sp. LB400 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification of the NADH: ferredoxin oxidoreductase component | Pseudomonas sp. |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -75°C, 4 months, 10% loss of activity | Pseudomonas sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| biphenyl + NAD(P)H + O2 | - |
Pseudomonas sp. | cis-biphenyl 2,3-dihydrodiol + NAD(P)+ | - |
? | |
| biphenyl + NAD(P)H + O2 | - |
Pseudomonas sp. LB400 | cis-biphenyl 2,3-dihydrodiol + NAD(P)+ | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 32 | - |
NADH: ferredoxin oxidoreductase component | Pseudomonas sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 55 | NADH: ferredoxin oxidoreductase component: no activity at 5 or 55°C, 15.2% of control activity at 50°C | Pseudomonas sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
NADH: ferredoxin oxidoreductase component | Pseudomonas sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Ferredoxin | - |
Pseudomonas sp. |
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