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Literature summary for 1.14.12.17 extracted from
- Functional implications of the proximal site hydrogen bonding network in Vitreoscilla hemoglobin (VHb): role of Tyr95 (G5) and Tyr126 (H12) (2008), FEBS Lett., 582, 3494-3500.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| plasmids pUC8 or pNKD1 are utilized for wild-type VHb expression in Escherichia coli JM109, DH5alpha and YCM10 cells, for cloning of the mutants the plasmid pVDX18 is used | Vitreoscilla |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Y126F | mutant, displays little change in oxygen affinity indicating a crucial role of Tyr95 in protein function | Vitreoscilla |
| Y126L | mutant, displays little change in oxygen affinity indicating a crucial role of Tyr95 in protein function | Vitreoscilla |
| Y95F | mutant, enzyme shows no stable oxyform and nitric oxide dioxygenase activity | Vitreoscilla |
| Y95L | mutant, enzyme shows no stable oxyform and nitric oxide dioxygenase activity | Vitreoscilla |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vitreoscilla | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
0.02 nmol of NO heme-1 s-1, Escherichia coli RB9096 | Vitreoscilla |
| additional information | - |
0.04 nmol of NO heme-1 s-1, Escherichia coli RB9096, VHb mutant Y95F | Vitreoscilla |
| additional information | - |
0.05 nmol of NO heme-1 s-1, Escherichia coli RB9096, VHb mutant Y95L | Vitreoscilla |
| additional information | - |
0.61 nmol of NO heme-1 s-1, Escherichia coli RB9096, VHb wild-type | Vitreoscilla |
| additional information | - |
0.86 nmol of NO heme-1 s-1, Escherichia coli RB9096, VHb mutant Y126F | Vitreoscilla |
| additional information | - |
0.94 nmol of NO heme-1 s-1, Escherichia coli RB9096, VHb mutant Y126L | Vitreoscilla |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| nitric oxide dioxygenase | - |
Vitreoscilla |
| NOD | - |
Vitreoscilla |
| VHb | - |
Vitreoscilla |
| Vitreoscilla hemoglobin | - |
Vitreoscilla |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| flavin | - |
Vitreoscilla |  |
| heme | - |
Vitreoscilla | |
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Release 2023.1 (January 2023)
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