Quinoline 2-oxidoreductase and 2-oxo-1,2-dihydroquinoline 5,6-dioxygenase from Comamonas testosteroni 63. The first two enzymes in quinoline and 3-methylquinoline degradation

Schach, S.; Tshisuaka, B.; Fetzner, S.; Lingens, F.; Eur. J. Biochem. 232, 536-544 (1995)

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

1,10-phenanthroline

0.5 mM, 82% loss of activity

Comamonas testosteroni

4-hydroxymercuribenzoate

0.1 mM, complete loss of activity

Comamonas testosteroni

Acriflavin

0.5 mM, 69% loss of activity

Comamonas testosteroni

Cu2+

-

Comamonas testosteroni

diethyldithiocarbaminate

inhibits after a prolonged incubation time

Comamonas testosteroni

EDTA

inhibits after a prolonged incubation time

Comamonas testosteroni

iodoacetate

2 mM, complete loss of activity

Comamonas testosteroni

NaCl

0.2 M, 50% loss of activity

Comamonas testosteroni

Quinacrine

0.5 mM, 15% loss of activity

Comamonas testosteroni

Metals/Ions

Commentary

Organism

Structure

Fe2+

enhances activity 1.5fold

Comamonas testosteroni

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

additional information

Comamonas testosteroni

second enzyme in the pathway of quinoline and 3-methylquinoline degradation

?

-

-

?

additional information

Comamonas testosteroni 63

second enzyme in the pathway of quinoline and 3-methylquinoline degradation

?

-

-

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

2-Oxo-1,2-dihydroquinoline + NADH + O2

-

7068

Comamonas testosteroni

?

-

-

-

?

2-Oxo-1,2-dihydroquinoline + NADH + O2

-

7068

Comamonas testosteroni 63

?

-

-

-

?

3-Methyl-2-oxo-1,2-dihydroquinoline + NADH + O2

NADH cannot be replaced by NADPH

7068

Comamonas testosteroni

5,6-Dihydro-5,6-dihydroxy-(3-methyl-)2-oxo-1,2-dihydroquinoline + NAD+

-

7068

Comamonas testosteroni

?

3-Methyl-2-oxo-1,2-dihydroquinoline + NADH + O2

NADH cannot be replaced by NADPH

7068

Comamonas testosteroni 63

5,6-Dihydro-5,6-dihydroxy-(3-methyl-)2-oxo-1,2-dihydroquinoline + NAD+

-

7068

Comamonas testosteroni 63

?

6-Hydroxy-2-oxo-1,2-dihydroquinoline + NADH + O2

-

7068

Comamonas testosteroni

?

-

-

-

?

6-Hydroxy-2-oxo-1,2-dihydroquinoline + NADH + O2

-

7068

Comamonas testosteroni 63

?

-

-

-

?

8-Hydroxy-2-oxo-1,2-dihydroquinoline + NADH + O2

-

7068

Comamonas testosteroni

?

-

-

-

?

8-Hydroxyquinoline + NADH + O2

-

7068

Comamonas testosteroni

?

-

-

-

?

8-Hydroxyquinoline + NADH + O2

-

7068

Comamonas testosteroni 63

?

-

-

-

?

additional information

second enzyme in the pathway of quinoline and 3-methylquinoline degradation

7068

Comamonas testosteroni

?

-

-

-

?

additional information

second enzyme in the pathway of quinoline and 3-methylquinoline degradation

7068

Comamonas testosteroni 63

?

-

-

-

?

Cofactor

Commentary

Organism

Structure

NADH

required, cannot be replaced by NADPH

Comamonas testosteroni

Cofactor

Commentary

Organism

Structure

NADH

required, cannot be replaced by NADPH

Comamonas testosteroni

Inhibitors

Commentary

Organism

Structure

1,10-phenanthroline

0.5 mM, 82% loss of activity

Comamonas testosteroni

4-hydroxymercuribenzoate

0.1 mM, complete loss of activity

Comamonas testosteroni

Acriflavin

0.5 mM, 69% loss of activity

Comamonas testosteroni

Cu2+

-

Comamonas testosteroni

diethyldithiocarbaminate

inhibits after a prolonged incubation time

Comamonas testosteroni

EDTA

inhibits after a prolonged incubation time

Comamonas testosteroni

iodoacetate

2 mM, complete loss of activity

Comamonas testosteroni

NaCl

0.2 M, 50% loss of activity

Comamonas testosteroni

Quinacrine

0.5 mM, 15% loss of activity

Comamonas testosteroni

Metals/Ions

Commentary

Organism

Structure

Fe2+

enhances activity 1.5fold

Comamonas testosteroni

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

additional information

Comamonas testosteroni

second enzyme in the pathway of quinoline and 3-methylquinoline degradation

?

-

-

?

additional information

Comamonas testosteroni 63

second enzyme in the pathway of quinoline and 3-methylquinoline degradation

?

-

-

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

2-Oxo-1,2-dihydroquinoline + NADH + O2

-

7068

Comamonas testosteroni

?

-

-

-

?

2-Oxo-1,2-dihydroquinoline + NADH + O2

-

7068

Comamonas testosteroni 63

?

-

-

-

?

3-Methyl-2-oxo-1,2-dihydroquinoline + NADH + O2

NADH cannot be replaced by NADPH

7068

Comamonas testosteroni

5,6-Dihydro-5,6-dihydroxy-(3-methyl-)2-oxo-1,2-dihydroquinoline + NAD+

-

7068

Comamonas testosteroni

?

3-Methyl-2-oxo-1,2-dihydroquinoline + NADH + O2

NADH cannot be replaced by NADPH

7068

Comamonas testosteroni 63

5,6-Dihydro-5,6-dihydroxy-(3-methyl-)2-oxo-1,2-dihydroquinoline + NAD+

-

7068

Comamonas testosteroni 63

?

6-Hydroxy-2-oxo-1,2-dihydroquinoline + NADH + O2

-

7068

Comamonas testosteroni

?

-

-

-

?

6-Hydroxy-2-oxo-1,2-dihydroquinoline + NADH + O2

-

7068

Comamonas testosteroni 63

?

-

-

-

?

8-Hydroxy-2-oxo-1,2-dihydroquinoline + NADH + O2

-

7068

Comamonas testosteroni

?

-

-

-

?

8-Hydroxyquinoline + NADH + O2

-

7068

Comamonas testosteroni

?

-

-

-

?

8-Hydroxyquinoline + NADH + O2

-

7068

Comamonas testosteroni 63

?

-

-

-

?

additional information

second enzyme in the pathway of quinoline and 3-methylquinoline degradation

7068

Comamonas testosteroni

?

-

-

-

?

additional information

second enzyme in the pathway of quinoline and 3-methylquinoline degradation

7068

Comamonas testosteroni 63

?

-

-

-

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

7068

Schach

Quinoline 2-oxidoreductase and ...

Comamonas testosteroni, Comamonas testosteroni 63

Eur. J. Biochem.

232

536-544

1995

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1

9

-

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1

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2

1

9

-

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1

-

-

-

1

-

11

-

-

-

-

2

-

1

-

-

1

-

-

-

-

-

-

1

-

-

1

-

9

-

-

-

1

-

2

1

-

-

1

-

-

1

-

11

-

-

-

2

-

1

-

-

-

-

-

-

-

-

-