Data extracted from this reference:
General Stability
dithioerythritol, dithiothreitol and 2-oxo-1,2-dihydroquinoline stabilize
Comamonas testosteroni
Inhibitors
1,10-phenanthroline
0.5 mM, 82% loss of activity
Comamonas testosteroni
4-hydroxymercuribenzoate
0.1 mM, complete loss of activity
Comamonas testosteroni
Acriflavin
0.5 mM, 69% loss of activity
Comamonas testosteroni
Cu2+
Comamonas testosteroni
diethyldithiocarbaminate
inhibits after a prolonged incubation time
Comamonas testosteroni
EDTA
inhibits after a prolonged incubation time
Comamonas testosteroni
iodoacetate
2 mM, complete loss of activity
Comamonas testosteroni
NaCl
0.2 M, 50% loss of activity
Comamonas testosteroni
Quinacrine
0.5 mM, 15% loss of activity
Comamonas testosteroni
Metals/Ions
Fe2+
enhances activity 1.5fold
Comamonas testosteroni
Natural Substrates/ Products (Substrates)
additional information
Comamonas testosteroni
second enzyme in the pathway of quinoline and 3-methylquinoline degradation
?
additional information
Comamonas testosteroni 63
second enzyme in the pathway of quinoline and 3-methylquinoline degradation
?
Organic Solvent Stability
Ethanol
5%, decreases enzyme activity to 44%
Comamonas testosteroni
Organism
Comamonas testosteroni 63
Purification (Commentary)
partial
Comamonas testosteroni
Specific Activity [micromol/min/mg]
0.125
Comamonas testosteroni
Substrates and Products (Substrate)
2-Oxo-1,2-dihydroquinoline + NADH + O2
7068
Comamonas testosteroni
?
2-Oxo-1,2-dihydroquinoline + NADH + O2
7068
Comamonas testosteroni 63
?
3-Methyl-2-oxo-1,2-dihydroquinoline + NADH + O2
NADH cannot be replaced by NADPH
7068
Comamonas testosteroni
5,6-Dihydro-5,6-dihydroxy-(3-methyl-)2-oxo-1,2-dihydroquinoline + NAD+
7068
Comamonas testosteroni
3-Methyl-2-oxo-1,2-dihydroquinoline + NADH + O2
NADH cannot be replaced by NADPH
7068
Comamonas testosteroni 63
5,6-Dihydro-5,6-dihydroxy-(3-methyl-)2-oxo-1,2-dihydroquinoline + NAD+
7068
Comamonas testosteroni 63
6-Hydroxy-2-oxo-1,2-dihydroquinoline + NADH + O2
7068
Comamonas testosteroni
?
6-Hydroxy-2-oxo-1,2-dihydroquinoline + NADH + O2
7068
Comamonas testosteroni 63
?
8-Hydroxy-2-oxo-1,2-dihydroquinoline + NADH + O2
7068
Comamonas testosteroni
?
8-Hydroxyquinoline + NADH + O2
7068
Comamonas testosteroni
?
8-Hydroxyquinoline + NADH + O2
7068
Comamonas testosteroni 63
?
additional information
second enzyme in the pathway of quinoline and 3-methylquinoline degradation
7068
Comamonas testosteroni
?
additional information
second enzyme in the pathway of quinoline and 3-methylquinoline degradation
7068
Comamonas testosteroni 63
?
Temperature Stability [°C]
30
5 min, 30% loss of activity
Comamonas testosteroni
65
5 min, complete loss of activity
Comamonas testosteroni
pH Optimum
7.3
Comamonas testosteroni
Cofactor
NADH
required, cannot be replaced by NADPH
Comamonas testosteroni
Cofactor (protein specific)
NADH
required, cannot be replaced by NADPH
Comamonas testosteroni
General Stability (protein specific)
dithioerythritol, dithiothreitol and 2-oxo-1,2-dihydroquinoline stabilize
Comamonas testosteroni
Inhibitors (protein specific)
1,10-phenanthroline
0.5 mM, 82% loss of activity
Comamonas testosteroni
4-hydroxymercuribenzoate
0.1 mM, complete loss of activity
Comamonas testosteroni
Acriflavin
0.5 mM, 69% loss of activity
Comamonas testosteroni
Cu2+
Comamonas testosteroni
diethyldithiocarbaminate
inhibits after a prolonged incubation time
Comamonas testosteroni
EDTA
inhibits after a prolonged incubation time
Comamonas testosteroni
iodoacetate
2 mM, complete loss of activity
Comamonas testosteroni
NaCl
0.2 M, 50% loss of activity
Comamonas testosteroni
Quinacrine
0.5 mM, 15% loss of activity
Comamonas testosteroni
Metals/Ions (protein specific)
Fe2+
enhances activity 1.5fold
Comamonas testosteroni
Natural Substrates/ Products (Substrates) (protein specific)
additional information
Comamonas testosteroni
second enzyme in the pathway of quinoline and 3-methylquinoline degradation
?
additional information
Comamonas testosteroni 63
second enzyme in the pathway of quinoline and 3-methylquinoline degradation
?
Organic Solvent Stability (protein specific)
Ethanol
5%, decreases enzyme activity to 44%
Comamonas testosteroni
Purification (Commentary) (protein specific)
partial
Comamonas testosteroni
Specific Activity [micromol/min/mg] (protein specific)
0.125
Comamonas testosteroni
Substrates and Products (Substrate) (protein specific)
2-Oxo-1,2-dihydroquinoline + NADH + O2
7068
Comamonas testosteroni
?
2-Oxo-1,2-dihydroquinoline + NADH + O2
7068
Comamonas testosteroni 63
?
3-Methyl-2-oxo-1,2-dihydroquinoline + NADH + O2
NADH cannot be replaced by NADPH
7068
Comamonas testosteroni
5,6-Dihydro-5,6-dihydroxy-(3-methyl-)2-oxo-1,2-dihydroquinoline + NAD+
7068
Comamonas testosteroni
3-Methyl-2-oxo-1,2-dihydroquinoline + NADH + O2
NADH cannot be replaced by NADPH
7068
Comamonas testosteroni 63
5,6-Dihydro-5,6-dihydroxy-(3-methyl-)2-oxo-1,2-dihydroquinoline + NAD+
7068
Comamonas testosteroni 63
6-Hydroxy-2-oxo-1,2-dihydroquinoline + NADH + O2
7068
Comamonas testosteroni
?
6-Hydroxy-2-oxo-1,2-dihydroquinoline + NADH + O2
7068
Comamonas testosteroni 63
?
8-Hydroxy-2-oxo-1,2-dihydroquinoline + NADH + O2
7068
Comamonas testosteroni
?
8-Hydroxyquinoline + NADH + O2
7068
Comamonas testosteroni
?
8-Hydroxyquinoline + NADH + O2
7068
Comamonas testosteroni 63
?
additional information
second enzyme in the pathway of quinoline and 3-methylquinoline degradation
7068
Comamonas testosteroni
?
additional information
second enzyme in the pathway of quinoline and 3-methylquinoline degradation
7068
Comamonas testosteroni 63
?
Temperature Stability [°C] (protein specific)
30
5 min, 30% loss of activity
Comamonas testosteroni
65
5 min, complete loss of activity
Comamonas testosteroni
pH Optimum (protein specific)
7.3
Comamonas testosteroni
Other publictions for EC 1.14.12.16
7068
Schach
Quinoline 2-oxidoreductase and ...
Comamonas testosteroni 63, Comamonas testosteroni
Eur. J. Biochem.
232
536-544
1995
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11
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