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Literature summary for 1.14.12.14 extracted from

  • Ruff, J.; Smits, T.H.; Cook, A.M.; Schleheck, D.
    Identification of two vicinal operons for the degradation of 2-aminobenzenesulfonate encoded on plasmid pSAH in Alcaligenes sp. strain O-1 (2010), Microbiol. Res., 165, 288-199.
    View publication on PubMed
Search for more literature for 1.14.12.14

Cloned(Commentary)

Cloned (Comment) Organism
gene absAa, DNA and amino acid sequence determination and analysis. Six genes of the abs cluster are co-transcribed and contain the genes, absAa and absAb, for two subunits of the oxygenase component of the ABS-dioxygenase system. Sequence comparisons, overview Alcaligenes sp. O-1
gene absAb, DNA and amino acid sequence determination and analysis. Six genes of the abs cluster are co-transcribed and contain the genes, absAa and absAb, for two subunits of the oxygenase component of the ABS-dioxygenase system. Sequence comparisons, overview Alcaligenes sp. O-1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-aminobenzenesulfonate + NADH + H+ + O2 Alcaligenes sp. O-1 2-aminobenzenesulfonate is used in the manufacture of dyes and pharmaceuticals, and, typical of xenobiotic aryl-sulfonates. The compound is degraded in few native environments, e.g. by Alcaligenes sp. strain O-1, the overall degradative process for ABS in strain O-1 is complex, overview. The pathway shows both split operons and dispersed pathway genes, and no transcriptional regulator 2,3-dihydroxybenzenesulfonate + NH3 + NAD+
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 ?

Organism

Organism UniProt Comment Textmining
Alcaligenes sp. O-1 Q9RBG4 AbsAb, enzyme component; DSM 6325, six genes of the abs cluster are co-transcribed and contain the genes for two subunits of the oxygenase component of the ABS-dioxygenase system
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Alcaligenes sp. O-1 Q9RBG5 AbsAa, enzyme component; DSM 6325, six genes of the abs cluster are co-transcribed and contain the genes for two subunits of the oxygenase component of the ABS-dioxygenase system
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzenesulfonate + NADH + H+ + O2 2-aminobenzenesulfonate is used in the manufacture of dyes and pharmaceuticals, and, typical of xenobiotic aryl-sulfonates. The compound is degraded in few native environments, e.g. by Alcaligenes sp. strain O-1, the overall degradative process for ABS in strain O-1 is complex, overview. The pathway shows both split operons and dispersed pathway genes, and no transcriptional regulator Alcaligenes sp. O-1 2,3-dihydroxybenzenesulfonate + NH3 + NAD+
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 ?
2-aminobenzenesulfonate + NADH + H+ + O2 i.e. orthanilate or ABS Alcaligenes sp. O-1 2,3-dihydroxybenzenesulfonate + NH3 + NAD+
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 ?

Subunits

Subunits Comment Organism
More the enzyme is composed of two components, AbsAa and AbsAb Alcaligenes sp. O-1

Synonyms

Synonyms Comment Organism
ABS-dioxygenase
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 Alcaligenes sp. O-1

Cofactor

Cofactor Comment Organism Structure
FAD part of the reductive component Alcaligenes sp. O-1
NADH
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 Alcaligenes sp. O-1

General Information

General Information Comment Organism
metabolism Alcaligenes sp. O-1 inducibly deaminates 2-aminobenzenesulfonate via dioxygenation to 3-sulfocatechol, which is desulfonated during meta ring-cleavage to yield 2-hydroxymuconate. This intermediate is transformed through the oxalocrotonate-branch of the sulfocatechol meta-pathway, overview. The pathway shows both split operons and dispersed pathway genes, and no transcriptional regulator, overview. The overall degradative process for ABS in strain O-1 is complex, overview Alcaligenes sp. O-1