Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.12.11 extracted from

  • Bagneris, C.; Cammack, R.; Mason, J.R.
    Subtle difference between benzene and toluene dioxygenases of Pseudomonas putida (2005), Appl. Environ. Microbiol., 71, 1570-1580.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.12.11

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 Pseudomonas putida
expressed in Escherichia coli strain JM109 Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ contains a [2Fe-2S] cluster Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
toluene + NADH + O2 Pseudomonas putida
-
 (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.045
-
 with benzene as substrate Pseudomonas putida
0.054
-
 with ethylbenzene as substrate Pseudomonas putida
0.056
-
 with toluene as substrate Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzene + NADH + O2
-
 Pseudomonas putida benzene dihydrodiol + NAD+
-
 ?
benzene + NADH + O2
-
 Pseudomonas putida benzene cis-dihydrodiol + NAD+
-
 ?
ethylbenzene + NADH + H+ + O2
-
 Pseudomonas putida ethylbenzene cis-dihydrodiol + NAD+
-
 ?
ethylbenzene + NADH + O2
-
 Pseudomonas putida ?
-
 ?
toluene + NADH + O2
-
 Pseudomonas putida (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?

Synonyms

Synonyms Comment Organism
ISPTOD
-
 Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Pseudomonas putida