Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | the oxygenase component contains 4-6 iron atoms per holoenzyme, the ferredoxin component contains one [2Fe-s2S] cluster of the Rieske type | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
three-component enzyme: 1. reductaseTOL, 2. ferredoxinTOL, 3. a complex iron-sulfur protein | Pseudomonas putida |
15300 | - |
alpha2beta2, 2 * 46000 + 2 * 15300, oxygenase component of toluene dioxygenase | Pseudomonas putida |
15500 | - |
1 * 15500, ferredoxinTOL component of the toluene dioxygenase, SDS-PAGE | Pseudomonas putida |
46000 | - |
1 * 46000, NADH-ferredoxinTOL reductase component, SDS-PAGE | Pseudomonas putida |
46000 | - |
alpha2beta2, 2 * 46000 + 2 * 15300, oxygenase component of toluene dioxygenase | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
F1 | - |
Purification (Comment) | Organism |
---|---|
ferredoxinTOL component of the toluene dioxygenase | Pseudomonas putida |
NADH-ferredoxinTOL reductase component of toluene dioxygenase | Pseudomonas putida |
oxygenase component, ISPTOL | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
toluene + NADH + O2 | - |
Pseudomonas putida | (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 15500, ferredoxinTOL component of the toluene dioxygenase, SDS-PAGE | Pseudomonas putida |
monomer | 1 * 46000, NADH-ferredoxinTOL reductase component, SDS-PAGE | Pseudomonas putida |
tetramer | alpha2beta2, 2 * 46000 + 2 * 15300, oxygenase component of toluene dioxygenase | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | NADH-ferredoxinTOL reductase component contains 1 mol of noncovalently bound FAD per mol of protein | Pseudomonas putida | |
NADH | - |
Pseudomonas putida |