Literature summary for 1.14.11.B22 extracted from

An, C.; Zhu, F.; Yao, Y.; Zhang, K.; Wang, W.; Zhang, J.; Wei, G.; Xia, Y.; Gao, Q.; Gao, S.
Beyond the cyclopropyl ring formation fungal Aj_EasH catalyzes asymmetric hydroxylation of ergot alkaloids (2022), Appl. Microbiol. Biotechnol., 106, 2981-2991.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli, GST-tagged enzyme	Aspergillus japonicus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Iron	-	Aspergillus japonicus

Organism

Organism	UniProt	Comment	Textmining
Aspergillus japonicus	A0A1L1QK38	bimodal dioxygenase that catalyzes both the formation of cyclopropyl ring and the asymmetric hydroxylation ergot alkaloids	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
9,10-dihydrolysergol + 2-oxoglutarate + O2	-	Aspergillus japonicus	8beta-(hydroxymethyl)-6-methylergolin-4-ol + succinate + CO2 + H2O	-	?
elymoclavine + 2-oxoglutarate + O2	-	Aspergillus japonicus	8-(hydroxymethyl)-6-methyl-8,9-didehydroergolin-4-ol + succinate + CO2 + H2O	-	?
festuclavine + 2-oxoglutarate + O2	asymmetric hydroxylation at the C-4 position of ergot alkaloid festuclavine	Aspergillus japonicus	6,8beta-dimethylergolin-4-ol + succinate + CO2 + H2O	-	?

Synonyms

Synonyms	Comment	Organism
EasH	bimodal dioxygenase that catalyzes both the formation of cyclopropyl ring and the asymmetric hydroxylation ergot alkaloids	Aspergillus japonicus

General Information

General Information	Comment	Organism
metabolism	EasH converts a six-membered enamine intermediate, generated by lavoenzyme EasA from chanocalvine-I aldehyde, to a cyclopropyl group-containing intermediate, which is reduced to cycloclavine by the nicotinamide adenine dinucleotide phosphate-dependent imine reductase EasG	Aspergillus japonicus
physiological function	EasH is a 2-oxoglutarate-dependent non-heme iron dioxygenase. EasH is a promiscuous and bimodal dioxygenase that catalyzes both the formation of cyclopropyl ring and the asymmetric hydroxylation ergot alkaloids	Aspergillus japonicus

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Release 2023.1 (January 2023)