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Literature summary for 1.14.11.9 extracted from
- Overexpression of a tea flavanone 3-hydroxylase gene confers tolerance to salt stress and Alternaria solani in transgenic tobacco (2014), Plant Mol. Biol., 85, 551-573 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of the Camellia sinensis flavanone 3-hydroxylase gene in leaves of transgenic Nicotiana tabacum cv. Xanthi confers tolerance to salt stress and pathogenic fungus Alternaria solani, transformation via Agrobacterium tumefaciens strain LBA4404 containing pCAMBIA-F3H. A decrease in pectin methyl esterase (PME) activity and increase in pectin methyl esterification is also observed in CsF3H transgenic tobacco plants. Transcript expression analysis, and analysis of trangenic PME activity in flavonoid (epigallocatechin)-exposed and -unexposed seedlings, and in seedlings exposed to salt | Camellia sinensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| a (2S)-flavan-4-one + 2-oxoglutarate + O2 | Camellia sinensis | - |
a (2R,3R)-dihydroflavonol + succinate + CO2 | - |
? |  |
| a (2S)-flavan-4-one + 2-oxoglutarate + O2 | Camellia sinensis UPASI-10 | - |
a (2R,3R)-dihydroflavonol + succinate + CO2 | - |
? | |
| eriodictyol + 2-oxoglutarate + O2 | Camellia sinensis | - |
dihydroquercetin + succinate + CO2 | - |
? | |
| eriodictyol + 2-oxoglutarate + O2 | Camellia sinensis UPASI-10 | - |
dihydroquercetin + succinate + CO2 | - |
? | |
| naringenin + 2-oxoglutarate + O2 | Camellia sinensis | - |
dihydrokaempferol + succinate + CO2 | - |
? | |
| naringenin + 2-oxoglutarate + O2 | Camellia sinensis UPASI-10 | - |
dihydrokaempferol + succinate + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Camellia sinensis | Q6DV45 | from the tea garden at the CSIR-Institute of Himalayan Bioresource Technology, Palampur | - |
| Camellia sinensis UPASI-10 | Q6DV45 | from the tea garden at the CSIR-Institute of Himalayan Bioresource Technology, Palampur | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Camellia sinensis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| a (2S)-flavan-4-one + 2-oxoglutarate + O2 | - |
Camellia sinensis | a (2R,3R)-dihydroflavonol + succinate + CO2 | - |
? | |
| a (2S)-flavan-4-one + 2-oxoglutarate + O2 | - |
Camellia sinensis UPASI-10 | a (2R,3R)-dihydroflavonol + succinate + CO2 | - |
? | |
| eriodictyol + 2-oxoglutarate + O2 | - |
Camellia sinensis | dihydroquercetin + succinate + CO2 | - |
? | |
| eriodictyol + 2-oxoglutarate + O2 | - |
Camellia sinensis UPASI-10 | dihydroquercetin + succinate + CO2 | - |
? | |
| naringenin + 2-oxoglutarate + O2 | - |
Camellia sinensis | dihydrokaempferol + succinate + CO2 | - |
? | |
| naringenin + 2-oxoglutarate + O2 | - |
Camellia sinensis UPASI-10 | dihydrokaempferol + succinate + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CsF3H | - |
Camellia sinensis |
| flavanone 3-hydroxylase | - |
Camellia sinensis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | flavanone 3-hydroxylase (F3H) of the flavonoid pathway catalyzes the stereospecific hydroxylation of (2S)-naringenin and (2S)-eriodictyol to form (2R,3R)-dihydrokaempferol and (2R,3R)-dihydroquercetin, respectively. These dihydroflavonols serve as intermediates for the biosynthesis of flavan-3-ols. Enzyme F3H plays a pivotal role in regulation of biosynthesis of flavan-3-ols in Camellia sinensis | Camellia sinensis |
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