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Literature summary for 1.14.11.9 extracted from

  • Chaipanya, C.; Saetiew, K.; Arunyanart, S.; Parinthawong, N.
    Isolation and expression analysis of the flavanone 3-hydroxylase genes in lotus (Nelumbo nucifera Gaertn.), waterlily (Nymphaea sp.) and transient silencing in waterlily (2017), Chiang Mai J. Sci., 44, 427-437 .
No PubMed abstract available
Search for more literature for 1.14.11.9

Cloned(Commentary)

Cloned (Comment) Organism
gene F3H, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis Nelumbo nucifera
gene F3H, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis Nymphaea hybrid cultivar

Protein Variants

Protein Variants Comment Organism
additional information method evaluation for RNAi-mediated silencing downregulation of F3H expression, introduction of RNAi gene-silencing vector, pJA8F3H, encoding a hairpin F3H RNA, to waterlily petals using the Agrobacterium tumefaciens strain GV3101 infiltration method, F3H expression is analysed at 1 and 3 days post infiltration semi-quantitative RT-PCR, showing that F3H expression is downregulated at 3 dpi in flowers tested of the red petal variety and purplish blue petal variety compared to controls. Vector pJA8F3H is efficient and can be used as a transformation vector to transiently suppress F3H expression in waterlily or lotus Nymphaea hybrid cultivar
additional information method evaluation for RNAi-mediated silencing downregulation of F3H expression, vector pJA8F3H is efficient and can be used as a transformation vector to transiently suppress F3H expression in waterlily or lotus Nelumbo nucifera

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a (2S)-flavan-4-one + 2-oxoglutarate + O2 Nelumbo nucifera
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 a (2R,3R)-dihydroflavonol + succinate + CO2
-
 ?
a (2S)-flavan-4-one + 2-oxoglutarate + O2 Nymphaea hybrid cultivar
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 a (2R,3R)-dihydroflavonol + succinate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Nelumbo nucifera V9P4G8 cv. Buntharik (white petal lotus) and cv. Satabankacha (pink petal lotus)
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Nymphaea hybrid cultivar V9P4H2 var. St. Louis Gold
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Source Tissue

Source Tissue Comment Organism Textmining
leaf low expression level Nymphaea hybrid cultivar
-
additional information expression of F3H is specifically regulated in petals and stamens. The F3H expression is slightly diminished in petals of cultivar Satabankacha at the fully-opening stage, whereas it is detected in the cultivar Buntharik only when white petals are tinted with pink. Nelumbo nucifera
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petal
-
 Nelumbo nucifera
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petal
-
 Nymphaea hybrid cultivar
-
stamen
-
 Nelumbo nucifera
-
stamen
-
 Nymphaea hybrid cultivar
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
 Nelumbo nucifera a (2R,3R)-dihydroflavonol + succinate + CO2
-
 ?
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
 Nymphaea hybrid cultivar a (2R,3R)-dihydroflavonol + succinate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
F3H
-
 Nelumbo nucifera
F3H
-
 Nymphaea hybrid cultivar
flavanone 3-hydroxylase
-
 Nelumbo nucifera
flavanone 3-hydroxylase
-
 Nymphaea hybrid cultivar

General Information

General Information Comment Organism
physiological function F3H is a key regulator for the flavonoid biosynthesis pathway and plays a role in accumulation of anthocyanin pigments in the vacuole Nelumbo nucifera
physiological function F3H is a key regulator for the flavonoid biosynthesis pathway and plays a role in accumulation of anthocyanin pigments in the vacuole Nymphaea hybrid cultivar