Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Petunia x hybrida |
Protein Variants | Comment | Organism |
---|---|---|
additional information | four C-terminally truncated enzyme forms are generated by deletion of five, 11, 24 or 29 amino acids. The recombinant enzymes preserve their substrate electrivity, but the specific activity decreases gradually with the extent of truncation. An enzyme chimera is constructed by domain swapping replacing the C-terminal 52 amino acids of the Petunia enzyme by equivalent region of flavonol synthase from Citrus unshiu | Petunia x hybrida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-flavanone + 2-oxoglutarate + O2 | Petunia x hybrida | key step towards biosynthesis of flavonols, anthocyanins and catechins | (2R/3R)-dihydroflavonol + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Petunia x hybrida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzymes | Petunia x hybrida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-flavanone + 2-oxoglutarate + O2 | key step towards biosynthesis of flavonols, anthocyanins and catechins | Petunia x hybrida | (2R/3R)-dihydroflavonol + succinate + CO2 | - |
? | |
eriodictyol + 2-oxoglutarate + O2 | 95% of the activity with naringenin | Petunia x hybrida | (2R,3R)-dihydroquercetin + succinate + CO2 | - |
? | |
naringenin + 2-oxoglutarate + O2 | - |
Petunia x hybrida | (2R,3R)-dihydrokaempferol + succinate + CO2 | - |
? | |
pinocembrin + 2-oxoglutarate + O2 | 38% of the activity with naringenin | Petunia x hybrida | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FHT | - |
Petunia x hybrida |
flavanone 3beta-hydroxylase | - |
Petunia x hybrida |