Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | required | Petunia x hybrida | |
catalase | stimulates | Petunia x hybrida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(+)-dihydrokaempferol | product inhibition | Petunia x hybrida | |
3-Bromo-2-oxoglutarate | 1 mM 92% inhibition | Petunia x hybrida | |
Cu2+ | - |
Petunia x hybrida | |
diethyldithiocarbamate | 2 mM, 81% inhibition | Petunia x hybrida | |
EDTA | 1 mM, 21% inhibition | Petunia x hybrida | |
Fe3+ | 1 mM, 56% inhibition | Petunia x hybrida | |
KCN | 1 mM, 11% inhibition | Petunia x hybrida | |
p-chloromercuribenzoate | 1 mM, 91% inhibition | Petunia x hybrida | |
Pyridine-2,4-dicarboxylate | 0.002 mM, 50% inhibition | Petunia x hybrida | |
Pyridine-2,5-dicarboxylate | 0.01 mM, 40% inhibition | Petunia x hybrida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | varying Km-values, depending on the purification procedure | Petunia x hybrida | |
0.0056 | - |
(2S)-naringenin | - |
Petunia x hybrida | |
0.012 | - |
(2S)-eriodictyol | - |
Petunia x hybrida | |
0.02 | - |
2-oxoglutarate | reaction with naringenin | Petunia x hybrida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can partially replace Fe2+ | Petunia x hybrida | |
Fe2+ | required | Petunia x hybrida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35000 + x * 37000, two-dimensional SDS-PAGE | Petunia x hybrida |
37000 | - |
x * 35000 + x * 37000, two-dimensional SDS-PAGE | Petunia x hybrida |
74000 | - |
gel filtration | Petunia x hybrida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Petunia x hybrida | - |
- |
- |
Oxidation Stability | Organism |
---|---|
partially stabilized under anaerobic conditions in presence of ascorbate | Petunia x hybrida |
Purification (Comment) | Organism |
---|---|
- |
Petunia x hybrida |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Petunia x hybrida |
Storage Stability | Organism |
---|---|
-70°C, in presence of 20 mM ascorbate, stable for more than 6 months | Petunia x hybrida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-naringenin + 2-oxoglutarate + O2 | - |
Petunia x hybrida | (2R,3R)-dihydrokaempferol + succinate + CO2 | - |
? | |
eriodictyol + 2-oxoglutarate + O2 | (2S)-eriodictyol | Petunia x hybrida | (2R,3R)-dihydroquercetin + succinate + CO2 | - |
? | |
additional information | no activity with (2R)-naringenin and 5,7,3',4',5'-pentahydroxyflavanone | Petunia x hybrida | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000 + x * 37000, two-dimensional SDS-PAGE | Petunia x hybrida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Petunia x hybrida |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
Pyridine 2,4-dicarboxylate | - |
Petunia x hybrida | |
0.04 | - |
Pyridine 2,5-dicarboxylate | - |
Petunia x hybrida |