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Literature summary for 1.14.11.9 extracted from

  • Britsch, L.; Grisebach, H.
    Purification and characterization of (2S)-flavanone 3-hydroxylase from Petunia hybrida (1986), Eur. J. Biochem., 156, 569-577.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ascorbate required Petunia x hybrida
catalase stimulates Petunia x hybrida

Inhibitors

Inhibitors Comment Organism Structure
(+)-dihydrokaempferol product inhibition Petunia x hybrida
3-Bromo-2-oxoglutarate 1 mM 92% inhibition Petunia x hybrida
Cu2+
-
Petunia x hybrida
diethyldithiocarbamate 2 mM, 81% inhibition Petunia x hybrida
EDTA 1 mM, 21% inhibition Petunia x hybrida
Fe3+ 1 mM, 56% inhibition Petunia x hybrida
KCN 1 mM, 11% inhibition Petunia x hybrida
p-chloromercuribenzoate 1 mM, 91% inhibition Petunia x hybrida
Pyridine-2,4-dicarboxylate 0.002 mM, 50% inhibition Petunia x hybrida
Pyridine-2,5-dicarboxylate 0.01 mM, 40% inhibition Petunia x hybrida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information varying Km-values, depending on the purification procedure Petunia x hybrida
0.0056
-
(2S)-naringenin
-
Petunia x hybrida
0.012
-
(2S)-eriodictyol
-
Petunia x hybrida
0.02
-
2-oxoglutarate reaction with naringenin Petunia x hybrida

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can partially replace Fe2+ Petunia x hybrida
Fe2+ required Petunia x hybrida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
x * 35000 + x * 37000, two-dimensional SDS-PAGE Petunia x hybrida
37000
-
x * 35000 + x * 37000, two-dimensional SDS-PAGE Petunia x hybrida
74000
-
gel filtration Petunia x hybrida

Organism

Organism UniProt Comment Textmining
Petunia x hybrida
-
-
-

Oxidation Stability

Oxidation Stability Organism
partially stabilized under anaerobic conditions in presence of ascorbate Petunia x hybrida

Purification (Commentary)

Purification (Comment) Organism
-
Petunia x hybrida

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Petunia x hybrida

Storage Stability

Storage Stability Organism
-70°C, in presence of 20 mM ascorbate, stable for more than 6 months Petunia x hybrida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S)-naringenin + 2-oxoglutarate + O2
-
Petunia x hybrida (2R,3R)-dihydrokaempferol + succinate + CO2
-
?
eriodictyol + 2-oxoglutarate + O2 (2S)-eriodictyol Petunia x hybrida (2R,3R)-dihydroquercetin + succinate + CO2
-
?
additional information no activity with (2R)-naringenin and 5,7,3',4',5'-pentahydroxyflavanone Petunia x hybrida ?
-
?

Subunits

Subunits Comment Organism
? x * 35000 + x * 37000, two-dimensional SDS-PAGE Petunia x hybrida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Petunia x hybrida

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0012
-
Pyridine 2,4-dicarboxylate
-
Petunia x hybrida
0.04
-
Pyridine 2,5-dicarboxylate
-
Petunia x hybrida