BRENDA - Enzyme Database show
show all sequences of 1.14.11.8

Fluorinated trimethyllysine as a (19)F NMR probe for trimethyllysine hydroxylase catalysis

Vijayendar Reddy, Y.; Al Temimi, A.H.; Mecinovic, J.; Org. Biomol. Chem. 15, 1350-1354 (2017)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
ascorbate
required
Homo sapiens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
dependent on, required for catalysis
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2
Homo sapiens
-
(3S)-3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
Q9NVH6
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
NMR spectrosopic structure analysis of substrates and products
745958
Homo sapiens
?
-
-
-
-
N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2
-
745958
Homo sapiens
(3S)-3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2
-
-
-
?
N6-fluoromethyl-N6,N6-dimethyl-L-lysine + 2-oxoglutarate + O2
Nepsilon-trimethyllysine analogue that contains the fluoromethyl group can be used as a 1H and 19F NMR probe for studies on TMLH catalysis
745958
Homo sapiens
(3S)-3-hydroxy-N6-fluoromethyl-N6,N6-dimethyl-L-lysine + succinate + CO2
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Homo sapiens
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
ascorbate
required
Homo sapiens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
dependent on, required for catalysis
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2
Homo sapiens
-
(3S)-3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
NMR spectrosopic structure analysis of substrates and products
745958
Homo sapiens
?
-
-
-
-
N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2
-
745958
Homo sapiens
(3S)-3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2
-
-
-
?
N6-fluoromethyl-N6,N6-dimethyl-L-lysine + 2-oxoglutarate + O2
Nepsilon-trimethyllysine analogue that contains the fluoromethyl group can be used as a 1H and 19F NMR probe for studies on TMLH catalysis
745958
Homo sapiens
(3S)-3-hydroxy-N6-fluoromethyl-N6,N6-dimethyl-L-lysine + succinate + CO2
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Homo sapiens
General Information
General Information
Commentary
Organism
metabolism
trimethyllysine hydroxylase (TMLH) catalyses C-3 hydroxylation of Nepsilon-trimethyllysine in the first step of carnitine biosynthesis in humans
Homo sapiens
physiological function
trimethyllysine hydroxylase (TMLH) catalyses C-3 hydroxylation of Nepsilon-trimethyllysine in the first step of carnitine biosynthesis in humans
Homo sapiens
General Information (protein specific)
General Information
Commentary
Organism
metabolism
trimethyllysine hydroxylase (TMLH) catalyses C-3 hydroxylation of Nepsilon-trimethyllysine in the first step of carnitine biosynthesis in humans
Homo sapiens
physiological function
trimethyllysine hydroxylase (TMLH) catalyses C-3 hydroxylation of Nepsilon-trimethyllysine in the first step of carnitine biosynthesis in humans
Homo sapiens
Other publictions for EC 1.14.11.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744681
Lesniak
Human carnitine biosynthesis ...
Homo sapiens
Chem. Commun. (Camb.)
53
440-442
2017
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1
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1
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1
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1
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-
-
-
2
2
-
-
-
745958
Vijayendar Reddy
Fluorinated trimethyllysine a ...
Homo sapiens
Org. Biomol. Chem.
15
1350-1354
2017
1
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-
-
-
-
-
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1
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1
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1
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1
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3
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1
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1
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2
2
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-
745961
Reddy
Evidence that trimethyllysine ...
Homo sapiens
Org. Lett.
19
400-403
2017
-
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-
-
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1
1
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1
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2
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1
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1
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-
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2
2
-
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-
744678
Al Temimi
Substrate scope for trimethyl ...
Homo sapiens
Chem. Commun. (Camb.)
52
12849-12852
2016
1
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1
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1
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2
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7
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7
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1
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1
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4
4
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746366
Kazaks
Expression and purification o ...
Homo sapiens
Protein Expr. Purif.
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1-6
2014
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1
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1
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1
1
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4
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2
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8
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2
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675029
Monfregola
Functional analysis of TMLH va ...
Homo sapiens, Mus musculus
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204
839-847
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4
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13
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2
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4
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2
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2
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2
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13
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2
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